Vu Thi Thu Hien, Tran Thi Viet Thanh, Dinh Thi Phong
Le Anh Tuan and Phi Hong Hai
Research Centre for Forest Tree Improvement
In this study, RAPD and cpDNA markers were used to assess genetic diversity of 20 Calocedrus macrolepis samples collected from three different locations (Ha Tay, Quang Binh, and Lam Dong). Among fifteen RAPD primers, there were 9 primers that revealed polymorphism with PIC value (Polymorphism Information Content) varying from 0.08 (RA40) to 0.31 (RA46), no primer had PIC value ≥ 0.5. A total of 56 DNA fragments were generated but only 22 DNA fragments showed polymorphism (approximately 39.2%).
Genetic similarity coefficients between samples ranged from 0.686 (QB3 and HT2; QB1 and LD6) to 1.0 (QB5 and QB6) and was used for constructing a phenetic dendrogram using UPGMA analysis. The pattern of grouping in the dendrogram divided 20 Calocedrus macrolepis samples into 3 main groups.
The first group includes 7 samples that were collected in Ha Tay, the second group is formed from 7 samples from Lam Dong and the third group involves 6 samples of Quang Binh province. Six primers of cpDNA were used to assess genetic diversity of 20 Calocedrus macrolepis samples there were four primer pairs that generated DNA fragments from 0.5 kb to 1.0 kb. However, non-polymorphic bands were generated, even digested by 9 different restriction enzymes.
Latest news
- Project: Research on selection and breeding of Acacia hybrid and Acacia auriculiformis for timber plantation in main ecological regions
- National seed orchard of Pinus merkusii
- National seed orchard of Acacia auriculiformis
- National seed orchard of Acacia mangium
- National seed orchard of Eucalyptus camaldulensis
Oldest news
- Establishment of pilot plantation of genetically improved Pinus merkusii having high resin productivity
- Breeding a number of native Bamboo specties in Northwestern Vietnam
- Results on genetic testing and vegetative propagation of Macadamia in Vietnam
- Research on the development of Ligustrum lucidum ait for urban greening
- Propagating garcinia cowa Roxb by grafting
