{"id":9732,"date":"2012-02-13T02:49:07","date_gmt":"2012-02-12T19:49:07","guid":{"rendered":"http:\/\/vafs.gov.vn\/vn\/2012\/02\/nghien-cuu-mot-so-yeu-to-anh-huong-den-chuyen-gen-gus-vao-phoi-truong-thanh-6-gia-dinh-thong-nhua-bang-vi-khuan-agrobacterium-tumefaciens\/"},"modified":"2013-10-06T09:02:34","modified_gmt":"2013-10-06T02:02:34","slug":"nghien-cuu-mot-so-yeu-to-anh-huong-den-chuyen-gen-gus-vao-phoi-truong-thanh-6-gia-dinh-thong-nhua-bang-vi-khuan-agrobacterium-tumefaciens","status":"publish","type":"post","link":"https:\/\/vafs.gov.vn\/vn\/nghien-cuu-mot-so-yeu-to-anh-huong-den-chuyen-gen-gus-vao-phoi-truong-thanh-6-gia-dinh-thong-nhua-bang-vi-khuan-agrobacterium-tumefaciens\/","title":{"rendered":"Nghi\u00ean c\u1ee9u m\u1ed9t s\u1ed1 y\u1ebfu t\u1ed1 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn chuy\u1ec3n gen GUS v\u00e0o ph\u00f4i tr\u01b0\u1edfng th\u00e0nh 6 gia \u0111\u00ecnh Th\u00f4ng nh\u1ef1a b\u1eb1ng vi khu\u1ea9n Agrobacterium tumefaciens"},"content":{"rendered":"

V\u01b0\u01a1ng \u0110\u00ecnh Tu\u1ea5n, Nguy\u1ec5n Xu\u00e2n C\u01b0\u1eddng, Phan Th\u1ecb M\u1ef5 Lan <\/strong> <\/p>\n

Ph\u00e2n vi\u1ec7n Nghi\u00ean c\u1ee9u Khoa h\u1ecdc L\u00e2m nghi\u1ec7p Nam B\u1ed9<\/em> <\/p>\n

\u0110\u1ed7 Ti\u1ebfn Ph\u00e1t, \u0110inh Th\u1ecb Ph\u00f2ng <\/strong> <\/p>\n

Vi\u1ec7n C\u00f4ng ngh\u1ec7 Sinh h\u1ecdc, H\u00e0 N\u1ed9i<\/em><\/strong> <\/p>\n

T\u00d3M T\u1eaeT<\/strong> <\/p>\n

M\u1ed9t s\u1ed1 y\u1ebfu t\u1ed1 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn chuy\u1ec3n gene GUS v\u00e0o ph\u00f4i tr\u01b0\u1edfng th\u00e0nh Th\u00f4ng nh\u1ef1a b\u1eb1ng vi khu\u1ea9n Agrobacterium tumefaciens<\/em> \u0111\u00e3 \u0111\u01b0\u1ee3c nghi\u00ean c\u1ee9u. K\u1ebft qu\u1ea3 cho th\u1ea5y 35mg\/l kanamycin c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ch\u1ecdn l\u1ecdc chuy\u1ec3n gene b\u1ec1n v\u1eefng, trong khi 400-500mg\/l Timentin c\u00f3 th\u1ec3 lo\u1ea1i vi khu\u1ea9n d\u01b0 sau \u0111\u1ed3ng nu\u00f4i c\u1ea5y c\u00f3 hi\u1ec7u qu\u1ea3 t\u1ed1t. N\u1ed3ng \u0111\u1ed9 c\u1ee7a vi khu\u1ea9n \u1edf OD600<\/sub> t\u1eeb 0,4-0,6 t\u1ecf ra c\u00f3 hi\u1ec7u qu\u1ea3 t\u1ed1t h\u01a1n c\u00e1c n\u1ed3ng \u0111\u1ed9 0,3 v\u00e0 0,8 trong chuy\u1ec3n gene GUS v\u00e0o ph\u00f4i th\u00f4ng v\u1edbi th\u1eddi gian \u0111\u1ed3ng nu\u00f4i c\u1ea5y trong kho\u1ea3ng 36 gi\u1edd. <\/p>\n

Th\u00e2n m\u1ea7m 4 ng\u00e0y tu\u1ed5i c\u0169ng c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c d\u00f9ng l\u00e0m v\u1eadt li\u1ec7u bi\u1ebfn n\u1ea1p gene GUS. Tuy nhi\u00ean t\u1ef7 l\u1ec7 s\u1ed1ng r\u1ea5t th\u1ea5p sau bi\u1ebfn n\u1ea1p. <\/p>\n

K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u n\u00e0y ch\u01b0a th\u1ec3 hi\u1ec7n hi\u1ec7u qu\u1ea3 c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p do vi\u1ec7c t\u1ea1o c\u00e2y xanh \u0111ang \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh nh\u01b0ng c\u0169ng \u0111\u00e3 b\u01b0\u1edbc \u0111\u1ea7u kh\u1eb3ng \u0111\u1ecbnh vi\u1ec7c chuy\u1ec3n gene GUS v\u00e0o ph\u00f4i tr\u01b0\u1edfng th\u00e0nh v\u00e0 cung c\u1ea5p m\u1ed9t s\u1ed1 th\u00f4ng s\u1ed1 ph\u1ee5c v\u1ee5 chuy\u1ec3n gene v\u00e0o Th\u00f4ng nh\u1ef1a b\u1eb1ng vi khu\u1ea9n Agrobacterium tumefaciens<\/em>.<\/strong> <\/p>\n

T\u1eeb kh\u00f3a: <\/strong>Chuy\u1ec3n gene b\u1eb1ng Agrobacterium tumefaciens<\/em>, gene GUS, Pinus merkusii.<\/strong><\/em><\/strong> <\/p>\n

M\u1ede \u0110\u1ea6U<\/strong> <\/p>\n

Nghi\u00ean c\u1ee9u bi\u1ebfn \u0111\u1ed5i gene trong c\u00e2y l\u00e2m nghi\u1ec7p \u0111\u00e3 \u0111\u01b0\u1ee3c tri\u1ec3n khai tr\u00ean 35 qu\u1ed1c gia, trong \u0111\u00f3 c\u00f3 16 qu\u1ed1c gia \u0111\u00e3 th\u1eed nghi\u1ec7m tr\u00ean hi\u1ec7n tr\u01b0\u1eddng. S\u1ed1 c\u00f2n l\u1ea1i, nh\u1eefng nghi\u00ean c\u1ee9u m\u1edbi \u0111\u01b0\u1ee3c tri\u1ec3n khai \u1edf m\u1ee9c \u0111\u1ed9 nh\u00e0 l\u01b0\u1edbi hay ph\u00f2ng th\u00ed nghi\u1ec7m. Hi\u1ec7n Trung Qu\u1ed1c l\u00e0 qu\u1ed1c gia c\u00f3 di\u1ec7n t\u00edch tr\u1ed3ng c\u00e2y D\u01b0\u01a1ng (Populus) chuy\u1ec3n gene l\u00ean t\u1edbi h\u01a1n m\u1ed9t tri\u1ec7u c\u00e2y (tr\u00ean 300ha). C\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u cho r\u1eb1ng c\u00e2y r\u1eebng bi\u1ebfn \u0111\u1ed5i gene c\u00f3 th\u1ec3 l\u00e0m t\u0103ng s\u1ea3n l\u01b0\u1ee3ng g\u1ed7 15%, t\u0103ng ch\u1ea5t l\u01b0\u1ee3ng g\u1ed7 12% gi\u1ea3m 9% thi\u1ec7t h\u1ea1i do s\u00e2u g\u00e2y ra v\u00e0 gi\u1ea3m 7% do b\u1ec7nh g\u00e2y ra. M\u1eb7t kh\u00e1c, c\u00e2y r\u1eebng bi\u1ebfn \u0111\u1ed5i gene c\u00f2n g\u00f3p ph\u1ea7n gi\u1ea3m gi\u00e1 th\u00e0nh ch\u1ebf bi\u1ebfn do gi\u1ea3m chi ph\u00ed h\u00f3a ch\u1ea5t kho\u1ea3ng 4%. \u0110\u1eb7c bi\u1ec7t, g\u00f3p ph\u1ea7n gi\u1ea3m \u00e1p l\u1ef1c l\u00ean r\u1eebng t\u1ef1 nhi\u00ean 12%,\u2026 (FAO 2006). Trong s\u1ed1 c\u00e1c lo\u00e0i c\u00e2y r\u1eebng chuy\u1ec3n gene, nghi\u00ean c\u1ee9u chuy\u1ec3n gene tr\u00ean h\u1ecd th\u00f4ng chi\u1ebfm 25%. \u1ede n\u01b0\u1edbc ta, nghi\u00ean c\u1ee9u chuy\u1ec3n gene tr\u00ean c\u00e2y r\u1eebng m\u1edbi \u0111\u01b0\u1ee3c b\u1eaft \u0111\u1ea7u t\u1eeb m\u1ea5y n\u0103m g\u1ea7n \u0111\u00e2y. Hi\u1ec7n nh\u1eefng k\u1ebft qu\u1ea3 chuy\u1ec3n gene tr\u00ean c\u00e2y r\u1eebng c\u00f2n r\u1ea5t h\u1ea1n ch\u1ebf, \u0111\u1eb7c bi\u1ec7t l\u00e0 ch\u01b0a c\u00f3 b\u1ea5t c\u1ee9 k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u n\u00e0o tr\u00ean lo\u00e0i Th\u00f4ng nh\u1ef1a (Pinus merkusii<\/em> Jungh & De Vries). Nghi\u00ean c\u1ee9u n\u00e0y nh\u1eb1m b\u01b0\u1edbc \u0111\u1ea7u t\u00ecm hi\u1ec3u m\u1ed9t s\u1ed1 th\u00f4ng s\u1ed1 ph\u1ee5c v\u1ee5 chuy\u1ec3n gene ch\u1ec9 th\u1ecb GUS v\u00e0o ph\u00f4i tr\u01b0\u1edfng th\u00e0nh Th\u00f4ng nh\u1ef1a b\u1eb1ng vi khu\u1ea9n Agrobacterium tumefaciens<\/em>. <\/p>\n

(K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u khoa h\u1ecdc c\u00f4ng ngh\u1ec7 giai \u0111o\u1ea1n 2006-2010, trang 60-66)<\/em> <\/p>\n","protected":false},"excerpt":{"rendered":"

\nM\u1ed9t s\u1ed1 y\u1ebfu t\u1ed1 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn chuy\u1ec3n gene GUS vào phôi tr\u01b0\u1edfng thành Thông nh\u1ef1a b\u1eb1ng vi khu\u1ea9n Agrobacterium tumefaciens<\/em> \u0111ã \u0111\u01b0\u1ee3c nghiên c\u1ee9u. K\u1ebft qu\u1ea3 cho th\u1ea5y 35mg\/l kanamycin có th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ch\u1ecdn l\u1ecdc chuy\u1ec3n gene b\u1ec1n v\u1eefng, trong khi 400-500mg\/l Timentin có th\u1ec3 lo\u1ea1i vi khu\u1ea9n d\u01b0 sau \u0111\u1ed3ng nuôi c\u1ea5y có hi\u1ec7u qu\u1ea3 t\u1ed1t. N\u1ed3ng \u0111\u1ed9 c\u1ee7a vi khu\u1ea9n \u1edf OD600<\/sub> t\u1eeb 0,4-0,6 t\u1ecf ra có hi\u1ec7u qu\u1ea3 t\u1ed1t h\u01a1n các n\u1ed3ng \u0111\u1ed9 0,3 và 0,8 trong chuy\u1ec3n gene GUS vào phôi thông v\u1edbi th\u1eddi gian \u0111\u1ed3ng nuôi c\u1ea5y trong kho\u1ea3ng 36 gi\u1edd. <\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[13],"tags":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/9732"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=9732"}],"version-history":[{"count":1,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/9732\/revisions"}],"predecessor-version":[{"id":11185,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/9732\/revisions\/11185"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=9732"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/categories?post=9732"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/tags?post=9732"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}