{"id":9165,"date":"2010-02-23T03:08:04","date_gmt":"2009-04-20T02:00:03","guid":{"rendered":"http:\/\/vafs.gov.vn\/vn\/2009\/04\/su-dung-phuong-phap-sinh-hoc-phan-tu-de-phat-hien-va-xac-dinh-nam-gay-benh-muc-go-bach-dan-eucalyptus-obliqua-l-her\/"},"modified":"2019-09-24T15:47:56","modified_gmt":"2019-09-24T08:47:56","slug":"su-dung-phuong-phap-sinh-hoc-phan-tu-de-phat-hien-va-xac-dinh-nam-gay-benh-muc-go-bach-dan-eucalyptus-obliqua-l-her","status":"publish","type":"post","link":"https:\/\/vafs.gov.vn\/vn\/su-dung-phuong-phap-sinh-hoc-phan-tu-de-phat-hien-va-xac-dinh-nam-gay-benh-muc-go-bach-dan-eucalyptus-obliqua-l-her\/","title":{"rendered":"S\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p sinh h\u1ecdc ph\u00e2n t\u1eed \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 x\u00e1c \u0111\u1ecbnh n\u1ea5m g\u00e2y b\u1ec7nh m\u1ee5c g\u1ed7 b\u1ea1ch \u0111\u00e0n Eucalyptus obliqua L. Her."},"content":{"rendered":"

Tr\u1ea7n Thanh Tr\u0103ng<\/strong><\/p>\n

Vi\u1ec7n Khoa h\u1ecdc L\u00e2m nghi\u1ec7p Vi\u00eat Nam<\/em><\/p>\n

T\u00d3M T\u1eaeT<\/strong><\/p>\n

Ph\u01b0\u01a1ng ph\u00e1p ph\u00e1t hi\u1ec7n v\u00e0 x\u00e1c \u0111\u1ecbnh n\u1ea5m g\u00e2y b\u1ec7nh m\u1ee5c g\u1ed7 b\u1ea1ch \u0111\u00e0n Eucalyptus obliqua<\/em> \u0111\u01b0\u1ee3c d\u1ef1a v\u00e0o ph\u00e2n t\u1eed DNA t\u00e1ch chi\u1ebft t\u1eeb 26 m\u1eabu g\u1ed7 b\u1ea1ch \u0111\u00e0n b\u1ecb m\u1ee5c thu th\u1eadp t\u1ea1i Tasmania (Australia). Ri-b\u00f4-x\u00f4m DNA \u0111\u01b0\u1ee3c khuy\u1ebfch \u0111\u1ea1i (PCR) b\u1edfi c\u1eb7p m\u1ed3i \u0111i\u1ec1n h\u00ecnh ITS1-F\/ITS4 ch\u1ec9 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c 18 m\u1eabu c\u00f3 DNA. Tr\u00ean b\u1ea3n gel cho th\u1ea5y c\u00f3 11 m\u1eabu ch\u1ec9 c\u00f3 1 d\u1ea3i DNA v\u00e0 7 m\u1eabu cho nhi\u1ec1u d\u1ea3i DNA. V\u00f9ng sao ch\u00e9p n\u1ed9i b\u1ed9 (ITS) c\u1ee7a 11 m\u1eabu \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh tr\u00ecnh t\u1ef1 chu\u1ed7i tr\u1ef1c ti\u1ebfp v\u00e0 7 m\u1eabu c\u00f3 s\u1ea3n ph\u1ea9m PCR th\u1ec3 hi\u1ec7n c\u00f3 nhi\u1ec1u lo\u00e0i n\u1ea5m c\u00f9ng xu\u1ea5t hi\u1ec7n \u0111\u01b0\u1ee3c t\u00e1ch d\u00f2ng. S\u1ea3n ph\u1ea9m t\u00e1ch d\u00f2ng \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p PCR-RFLP, \u0111\u1ea1i di\u1ec7n c\u1ee7a m\u1ed7i nh\u00f3m PCR-RFLP \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh tr\u00ecnh t\u1ef1 chu\u1ed7i. \u0110\u1ec3 x\u00e1c \u0111\u1ecbnh c\u00e1c lo\u00e0i n\u1ea5m m\u1ee5c g\u1ed7 trong m\u1eabu th\u00ed nghi\u1ec7m, c\u00e1c chu\u1ed7i ITS \u0111\u01b0\u1ee3c so s\u00e1nh ho\u1eb7c ph\u00e2n t\u00edch s\u1ef1 ti\u1ebfn h\u00f3a v\u1edbi c\u00e1c c\u01a1 s\u1edf d\u1eef li\u1ec7u tr\u00ean ng\u00e2n h\u00e0ng gen (Genbank) v\u00e0 c\u00e1c c\u01a1 s\u1edf d\u1eef li\u1ec7u c\u00e1 nh\u00e2n. K\u1ebft qu\u1ea3 ph\u00e2n t\u00edch DNA t\u1eeb c\u00e1c m\u1eabu g\u1ed7 m\u1ee5c \u0111\u00e3 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c 15 lo\u00e0i n\u1ea5m thu\u1ed9c l\u1edbp Basidiomycetes, 17 lo\u00e0i n\u1ea5m thu\u1ed9c l\u1edbp Ascomycetes v\u00e0 1 lo\u00e0i thu\u1ed9c l\u1edbp Zygomycetes. Trong t\u1ed5ng s\u1ed1 33 lo\u00e0i n\u1ea5m ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c trong m\u1eabu g\u1ed7, ch\u1ec9 c\u00f3 13 lo\u00e0i thu\u1ed9c l\u1edbp n\u1ea5m Basidiomycetes v\u00e0 7 lo\u00e0i thu\u1ed9c l\u1edbp n\u1ea5m Ascomycetes l\u00e0 nh\u1eefng lo\u00e0i g\u00e2y m\u1ee5c g\u1ed7 th\u1ef1c s\u1ef1, c\u00f2n 13 lo\u00e0i kh\u00e1c l\u00e0 c\u00e1c lo\u00e0i th\u1ee9 sinh.<\/p>\n

T\u1eeb kh\u00f3a<\/strong>: B\u1ea1ch \u0111\u00e0n Eucalyptus obliqua, DNA, n\u1ea5m g\u00e2y m\u1ee5c g\u1ed7, PCR-RFLP<\/em>.<\/p>\n

Ngu\u1ed3n: T\u1ea1p ch\u00ed N\u00f4ng nghi\u1ec7p v\u00e0 Ph\u00e1t tri\u1ec3n N\u00f4ng th\u00f4n s\u1ed1 117-2007, trang 59-64<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"

\n \n<\/p>\n

\nPh\u01b0\u01a1ng pháp phát hi\u1ec7n và xác \u0111\u1ecbnh n\u1ea5m gây b\u1ec7nh m\u1ee5c g\u1ed7 b\u1ea1ch \u0111àn Eucalyptus obliqua<\/em> \u0111\u01b0\u1ee3c d\u1ef1a vào phân t\u1eed DNA tách chi\u1ebft t\u1eeb 26 m\u1eabu g\u1ed7 b\u1ea1ch \u0111àn b\u1ecb m\u1ee5c thu th\u1eadp t\u1ea1i Tasmania (Australia). Ri-bô-xôm DNA \u0111\u01b0\u1ee3c khuy\u1ebfch \u0111\u1ea1i (PCR) b\u1edfi c\u1eb7p m\u1ed3i \u0111i\u1ec1n hình ITS1-F\/ITS4 ch\u1ec9 phát hi\u1ec7n \u0111\u01b0\u1ee3c 18 m\u1eabu có DNA. Trên b\u1ea3n gel cho th\u1ea5y có 11 m\u1eabu ch\u1ec9 có 1 d\u1ea3i DNA và 7 m\u1eabu cho nhi\u1ec1u d\u1ea3i DNA. Vùng sao chép n\u1ed9i b\u1ed9 (ITS) c\u1ee7a 11 m\u1eabu \u0111\u01b0\u1ee3c xác \u0111\u1ecbnh trình t\u1ef1 chu\u1ed7i tr\u1ef1c ti\u1ebfp và 7 m\u1eabu có s\u1ea3n ph\u1ea9m PCR th\u1ec3 hi\u1ec7n có nhi\u1ec1u loài n\u1ea5m cùng xu\u1ea5t hi\u1ec7n \u0111\u01b0\u1ee3c tách dòng. S\u1ea3n ph\u1ea9m tách dòng \u0111\u01b0\u1ee3c phân tích b\u1eb1ng ph\u01b0\u01a1ng pháp PCR-RFLP, \u0111\u1ea1i di\u1ec7n c\u1ee7a m\u1ed7i nhóm PCR-RFLP \u0111\u01b0\u1ee3c xác \u0111\u1ecbnh trình t\u1ef1 chu\u1ed7i. \u0110\u1ec3 xác \u0111\u1ecbnh các loài n\u1ea5m m\u1ee5c g\u1ed7 trong m\u1eabu thí nghi\u1ec7m, các chu\u1ed7i ITS \u0111\u01b0\u1ee3c so sánh ho\u1eb7c phân tích s\u1ef1 ti\u1ebfn hóa v\u1edbi các c\u01a1 s\u1edf d\u1eef li\u1ec7u trên ngân hàng gen (Genbank) và các c\u01a1 s\u1edf d\u1eef li\u1ec7u cá nhân. K\u1ebft qu\u1ea3 phân tích DNA t\u1eeb các m\u1eabu g\u1ed7 m\u1ee5c \u0111ã xác \u0111\u1ecbnh \u0111\u01b0\u1ee3c 15 loài n\u1ea5m thu\u1ed9c l\u1edbp Basidiomycetes, 17 loài n\u1ea5m thu\u1ed9c l\u1edbp Ascomycetes và 1 loài thu\u1ed9c l\u1edbp Zygomycetes. <\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[13],"tags":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/9165"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=9165"}],"version-history":[{"count":4,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/9165\/revisions"}],"predecessor-version":[{"id":19194,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/9165\/revisions\/19194"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=9165"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/categories?post=9165"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/tags?post=9165"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}