{"id":8892,"date":"2006-08-14T00:29:17","date_gmt":"2006-08-13T17:29:17","guid":{"rendered":"http:\/\/vafs.gov.vn\/vn\/2006\/08\/ket-qua-nghien-cuu-ung-dung-cong-nghe-rhizobium-cho-keo-lai-keo-tai-tuong-tai-vuon-uom-va-rung-trong\/"},"modified":"2019-09-24T15:39:29","modified_gmt":"2019-09-24T08:39:29","slug":"ket-qua-nghien-cuu-ung-dung-cong-nghe-rhizobium-cho-keo-lai-keo-tai-tuong-tai-vuon-uom-va-rung-trong","status":"publish","type":"post","link":"https:\/\/vafs.gov.vn\/vn\/ket-qua-nghien-cuu-ung-dung-cong-nghe-rhizobium-cho-keo-lai-keo-tai-tuong-tai-vuon-uom-va-rung-trong\/","title":{"rendered":"K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u \u1ee9ng d\u1ee5ng C\u00f4ng ngh\u1ec7 RHIZOBIUM cho keo lai, keo tai t\u01b0\u1ee3ng t\u1ea1i v\u01b0\u1eddn \u01b0\u01a1m v\u00e0 r\u1eebng tr\u1ed3ng"},"content":{"rendered":"

L\u00ea Qu\u1ed1c Huy <\/b><\/p>\n

Nguy\u1ec5n Minh Ch\u00e2u <\/b><\/p>\n

Trung t\u00e2m Nghi\u00ean c\u1ee9u Sinh th\u00e1i v\u00e0 M\u00f4i tr\u01b0\u1eddng r\u1eebng<\/i><\/p>\n

Vi\u1ec7n Khoa h\u1ecdc L\u00e2m nghi\u1ec7p Vi\u1ec7t Nam<\/i><\/p>\n

<\/h1>\n

M\u1edf \u0111\u1ea7u<\/b><\/p>\n

H\u00e0ng n\u0103m tr\u00ean to\u00e0n th\u1ebf gi\u1edbi c\u00f3 kho\u1ea3ng 120- 160 tri\u1ec7u t\u1ea5n Nit\u01a1 kh\u00ed quy\u1ec3n \u0111\u01b0\u1ee3c c\u1ed1 \u0111\u1ecbnh v\u00e0 chuy\u1ec3n ho\u00e1 th\u00e0nh ngu\u1ed3n \u0111\u1ea1m d\u01b0\u1edbi c\u00e1c d\u1ea1ng kh\u00e1c nhau th\u00f4ng qua qu\u00e1 tr\u00ecnh c\u1ed1 \u0111\u1ecbnh \u0111\u1ea1m sinh h\u1ecdc t\u1ef1 nhi\u00ean, (Gibson, 1995). L\u01b0\u1ee3ng \u0111\u1ea1m n\u00e0y \u01b0\u1edbc t\u00ednh g\u1ea5p kho\u1ea3ng 2 l\u1ea7n l\u01b0\u1ee3ng ph\u00e2n nit\u01a1 ho\u00e1 h\u1ecdc s\u1ea3n xu\u1ea5t ra h\u00e0ng n\u0103m tr\u00ean th\u1ebf gi\u1edbi. Th\u00f4ng qua c\u1ed9ng sinh gi\u1eefa vi khu\u1ea9n rhizobium v\u1edbi r\u1ec5 c\u00e1c c\u00e2y h\u1ecd \u0111\u1eadu, qu\u00e1 tr\u00ecnh c\u1ed1 \u0111\u1ecbnh \u0111\u1ea1m sinh h\u1ecdc \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n, c\u00e2y ch\u1ee7 s\u1ebd l\u1ea5y \u0111\u01b0\u1ee3c ngu\u1ed3n \u0111\u1ea1m v\u00f4 c\u01a1 cho sinh tr\u01b0\u1edfng, ng\u01b0\u1ee3c l\u1ea1i vi khu\u1ea9n s\u1ebd c\u00f3 \u0111\u01b0\u1ee3c c\u00e1c ngu\u1ed3n hydrad cacbon (\u0111\u01b0\u1eddng, tinh b\u1ed9t) cho ho\u1ea1t \u0111\u1ed9ng s\u1ed1ng. Quan h\u1ec7 c\u1ed9ng sinh n\u00e0y c\u00f3 vai tr\u00f2 quan tr\u1ecdng trong chu tr\u00ecnh dinh d\u01b0\u1ee1ng nit\u01a1, \u1ed5n \u0111\u1ecbnh n\u0103ng su\u1ea5t c\u00e2y tr\u1ed3ng v\u00e0 b\u1ec1n v\u1eefng h\u1ec7 sinh th\u00e1i (Macdicken, 1994. Balasundaran, 1995). L\u0129nh v\u1ef1c c\u1ed9ng ngh\u1ec7 vi khu\u1ea9n c\u1ed1 \u0111\u1ecbnh \u0111\u1ea1m rhizobium \u0111ang \u0111\u01b0\u1ee3c quan t\u00e2m, thu h\u00fat nhi\u1ec1u nghi\u00ean c\u1ee9u v\u00e0 \u00e1p d\u1ee5ng cho nhi\u1ec1u c\u00e2y tr\u1ed3ng kh\u00e1c nhau. Keo lai v\u00e0 Keo tai t\u01b0\u1ee3ng l\u00e0 2 lo\u00e0i c\u00e2y tr\u1ed3ng quan tr\u1ecdng c\u1ee7a Ch\u01b0\u01a1ng tr\u00ecnh 5 tri\u1ec7u ha r\u1eebng v\u00e0 nhi\u1ec1u ch\u01b0\u01a1ng tr\u00ecnh tr\u1ed3ng r\u1eebng kh\u00e1c \u1edf Vi\u1ec7t Nam. K\u1ef9 thu\u1eadt tuy\u1ec3n ch\u1ecdn, s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m rhizobium c\u00f3 hi\u1ec7u l\u1ef1c c\u1ed9ng sinh cao \u1ee9ng d\u1ee5ng cho keo lai, keo tai t\u01b0\u1ee3ng v\u01b0\u1eddn \u01b0\u01a1m v\u00e0 r\u1eebng tr\u1ed3ng s\u1ebd g\u00f3p ph\u1ea7n l\u00e0m t\u0103ng ch\u1ea5t l\u01b0\u1ee3ng, hi\u1ec7u qu\u1ea3 s\u1ea3n xu\u1ea5t c\u00e2y con v\u00e0 n\u0103ng su\u1ea5t r\u1eebng tr\u1ed3ng.<\/p>\n

<\/h1>\n

V\u1eadt li\u1ec7u v\u00e0 ph\u01b0\u01a1ng ph\u00e1p nghi\u00ean c\u1ee9u c\u1ee7a \u0111\u1ec1 <\/b>t\u00e0i<\/b><\/p>\n

K\u1ef9 thu\u1eadt ph\u00e2n l\u1eadp & tuy\u1ec3n ch\u1ecdn c\u00e1c ch\u1ee7ng rhizobium c\u00f3 hi\u1ec7u l\u1ef1c c\u1ed9ng sinh cao <\/i><\/b><\/a><\/i><\/p>\n

C\u00e1c ch\u1ee7ng vi khu\u1ea9n rhizobium \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp t\u1eeb n\u1ed1t s\u1ea7n r\u1ec5 c\u1ee7a c\u00e2y ch\u1ee7 keo lai v\u00e0 tai t\u01b0\u1ee3ng sinh tr\u01b0\u1edfng tr\u00ean m\u1ed9t s\u1ed1 v\u00f9ng sinh th\u00e1i & l\u1eadp \u0111\u1ecba kh\u00e1c nhau. Qu\u00e1 tr\u00ecnh ph\u00e2n l\u1eadp \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh tr\u00ean m\u00f4i tr\u01b0\u1eddng YMA (Yeast Manitol Agar), YMA- Red Congo & YMA- Bromthymol Blue (Somasegaran v\u00e0 Hoben, 1985), qua \u0111\u00f3 c\u00e1c ch\u1ee7ng \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp & ph\u00e2n lo\u1ea1i ra 2 nh\u00f3m l\u00e0 Rhizobium<\/i> sinh tr\u01b0\u1edfng nhanh, ph\u1ea3n \u1ee9ng acid m\u00e0u v\u00e0ng v\u00e0 nh\u00f3m Brandyrhizobum<\/i> sinh tr\u01b0\u1edfng ch\u1eadm, ph\u1ea3n \u1ee9ng ki\u1ec1m m\u00e0u xanh tr\u00ean m\u00f4i tr\u01b0\u1eddng tr\u00ean m\u00f4i tr\u01b0\u1eddng YMA- Bromthymol Blue. C\u00e1c ch\u1ee7ng ph\u00e2n l\u1eadp sau \u0111\u00f3 \u0111\u01b0\u1ee3c tuy\u1ec3n ch\u1ecdn qua nhi\u1ec1u b\u01b0\u1edbc, tr\u01b0\u1edbc h\u1ebft l\u00e0 kh\u1ea3 n\u0103ng h\u00ecnh th\u00e0nh c\u1ed9ng sinh n\u1ed1t s\u1ea7n v\u1edbi c\u00e2y ch\u1ee7 trong \u0111i\u1ec1u ki\u1ec7n in-vitro b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p C\u1ea7u gi\u1ea5y- \u1ed1ng nghi\u1ec7m tr\u00ean m\u00f4i tr\u01b0\u1eddng SNS (Seedling Nutrition Solution) (Gibson 1963, 1995, Vincent 1970, Somasegaran & Hoben 1985); ch\u1ec9 ti\u00eau \u0111\u00e1nh gi\u00e1 l\u00e0 s\u1ed1 l\u01b0\u1ee3ng v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng n\u1ed1t s\u1ea7n h\u00ecnh th\u00e0nh.<\/p>\n

B\u01b0\u1edbc ti\u1ebfp theo l\u00e0 tuy\u1ec3n ch\u1ecdn hi\u1ec7u l\u1ef1c c\u1ed9ng sinh v\u1edbi c\u00e2y ch\u1ee7 trong \u0111i\u1ec1u ki\u1ec7n nh\u00e0 k\u00ednh b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p b\u00ecnh Leonard tr\u00ean m\u00f4i tr\u01b0\u1eddng SNS v\u00f4 \u0111\u1ea1m (Nitrogen-free nutrient solution) (Vincent 1970, Somasegaran & Hoben 1985). C\u00e2y ch\u1ee7 \u0111\u01b0\u1ee3c nhi\u1ec5m rhizobium v\u00e0 sinh tr\u01b0\u1edfng trong \u0111i\u1ec1u ki\u1ec7n th\u00ed nghi\u1ec7m 8-10 tu\u1ea7n, ch\u1ec9 c\u00e2y n\u00e0o h\u00ecnh th\u00e0nh \u0111\u01b0\u1ee3c c\u1ed9ng sinh n\u1ed1t s\u1ea7n v\u1edbi c\u00e1c ch\u1ee7ng rhizobium nhi\u1ec5m, th\u00ec ch\u00fang m\u1edbi sinh tr\u01b0\u1edfng t\u1ed1t, b\u1eb1ng kh\u00f4ng sinh tr\u01b0\u1edfng s\u1ebd k\u00e9m. Ch\u1ec9 ti\u00eau \u0111\u00e1nh gi\u00e1 l\u00e1 s\u1ed1 l\u01b0\u1ee3ng, tr\u1ecdng l\u01b0\u1ee3ng n\u1ed1t s\u1ea7n h\u00ecnh th\u00e0nh, chi\u1ec1u cao, \u0111\u01b0\u1eddng k\u00ednh v\u00e0 sinh kh\u1ed1i c\u1ee7a c\u00e2y ch\u1ee7. C\u00e1c ch\u1ee7ng \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 l\u00e0 c\u00f3 hi\u1ec7u l\u1ef1c c\u1ed9ng sinh cao trong th\u00ed nghi\u1ec7m b\u00ecnh Leonard s\u1ebd \u0111\u01b0\u1ee3c tuy\u1ec3n ch\u1ecdn hi\u1ec7u l\u1ef1c ti\u1ebfp theo trong \u0111i\u1ec1u ki\u1ec7n th\u00ed nghi\u1ec7m v\u01b0\u1eddn \u01b0\u01a1m v\u1edbi th\u00e0nh ph\u1ea7n l\u00f5i b\u1ea7u l\u00e0 \u0111\u1ea5t c\u1ee7a hi\u1ec7n tr\u01b0\u1eddng \u00e1p d\u1ee5ng, ch\u1ec9 ti\u00eau \u0111\u00e1nh gi\u00e1 l\u00e0 s\u1ed1 l\u01b0\u1ee3ng, tr\u1ecdng l\u01b0\u1ee3ng n\u1ed1t s\u1ea7n, chi\u1ec1u cao, \u0111\u01b0\u1eddng k\u00ednh v\u00e0 sinh kh\u1ed1i.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

H\u00e0ng n\u0103m tr\u00ean to\u00e0n th\u1ebf gi\u1edbi c\u00f3 kho\u1ea3ng 120- 160 tri\u1ec7u t\u1ea5n Nit\u01a1 kh\u00ed quy\u1ec3n \u0111\u01b0\u1ee3c c\u1ed1 \u0111\u1ecbnh v\u00e0 chuy\u1ec3n ho\u00e1 th\u00e0nh ngu\u1ed3n \u0111\u1ea1m d\u01b0\u1edbi c\u00e1c d\u1ea1ng kh\u00e1c nhau th\u00f4ng qua qu\u00e1 tr\u00ecnh c\u1ed1 \u0111\u1ecbnh \u0111\u1ea1m sinh h\u1ecdc t\u1ef1 nhi\u00ean, (Gibson, 1995). L\u01b0\u1ee3ng \u0111\u1ea1m n\u00e0y \u01b0\u1edbc t\u00ednh g\u1ea5p kho\u1ea3ng 2 l\u1ea7n l\u01b0\u1ee3ng ph\u00e2n nit\u01a1 ho\u00e1 h\u1ecdc s\u1ea3n xu\u1ea5t ra h\u00e0ng n\u0103m tr\u00ean th\u1ebf gi\u1edbi. Th\u00f4ng qua c\u1ed9ng sinh gi\u1eefa vi khu\u1ea9n rhizobium v\u1edbi r\u1ec5 c\u00e1c c\u00e2y h\u1ecd \u0111\u1eadu, qu\u00e1 tr\u00ecnh c\u1ed1 \u0111\u1ecbnh \u0111\u1ea1m sinh h\u1ecdc \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n, c\u00e2y ch\u1ee7 s\u1ebd l\u1ea5y \u0111\u01b0\u1ee3c ngu\u1ed3n \u0111\u1ea1m v\u00f4 c\u01a1 cho sinh tr\u01b0\u1edfng, ng\u01b0\u1ee3c l\u1ea1i vi khu\u1ea9n s\u1ebd c\u00f3 \u0111\u01b0\u1ee3c c\u00e1c ngu\u1ed3n hydrad cacbon (\u0111\u01b0\u1eddng, tinh b\u1ed9t) cho ho\u1ea1t \u0111\u1ed9ng s\u1ed1ng. Quan h\u1ec7 c\u1ed9ng sinh n\u00e0y c\u00f3 vai tr\u00f2 quan tr\u1ecdng trong chu tr\u00ecnh dinh d\u01b0\u1ee1ng nit\u01a1, \u1ed5n \u0111\u1ecbnh n\u0103ng su\u1ea5t c\u00e2y tr\u1ed3ng v\u00e0 b\u1ec1n v\u1eefng h\u1ec7 sinh th\u00e1i (Macdicken, 1994. Balasundaran, 1995).<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[21],"tags":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/8892"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=8892"}],"version-history":[{"count":2,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/8892\/revisions"}],"predecessor-version":[{"id":19176,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/8892\/revisions\/19176"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=8892"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/categories?post=8892"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/tags?post=8892"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}