{"id":8885,"date":"2006-06-16T03:25:38","date_gmt":"2006-06-15T20:25:38","guid":{"rendered":"http:\/\/vafs.gov.vn\/vn\/2006\/06\/kha-nang-tai-sinh-choi-in-vitro-o-cay-do-tram-aquilari-crassna-pierre\/"},"modified":"2013-10-06T09:00:45","modified_gmt":"2013-10-06T02:00:45","slug":"kha-nang-tai-sinh-choi-in-vitro-o-cay-do-tram-aquilari-crassna-pierre","status":"publish","type":"post","link":"https:\/\/vafs.gov.vn\/vn\/kha-nang-tai-sinh-choi-in-vitro-o-cay-do-tram-aquilari-crassna-pierre\/","title":{"rendered":"Kh\u1ea3 n\u0103ng t\u00e1i sinh ch\u1ed3i in vitro \u1edf c\u00e2y D\u00f3 tr\u1ea7m Aquilari crassna. Pierre"},"content":{"rendered":"\n

T\u1ea1 Minh Ho\u00e0 – Ch\u1ee7 nhi\u1ec7m \u0111\u1ec1 t\u00e0i<\/i> <\/p>\n

Nguy\u1ec5n th\u1ecb Hi\u1ec1n – C\u1ed9ng t\u00e1c vi\u00ean \u0111\u1ec1 t\u00e0i <\/i> <\/p>\n

” H\u1ee3p t\u00e1c nghi\u00ean c\u1ee9u n\u00e2ng cao hi\u1ec7u qu\u1ea3 nh\u00e2n gi\u1ed1ng c\u00e2y D\u00f3 tr\u1ea7m b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p c\u00f4ng ngh\u1ec7 nu\u00f4i c\u1ea5y m\u00f4 t\u1ebf b\u00e0o th\u1ef1c v\u1eadt”<\/i> <\/p>\n

T<\/b> rung t\u00e2m nghi\u00ean c\u1ee9u l\u00e2m \u0111\u1eb7c s\u1ea3n<\/b> <\/p>\n

<\/i><\/i> <\/p>\n

T\u00f3m t\u1eaft<\/b> <\/u><\/i>: S\u1ef1 nu\u00f4i c\u1ea5y Aquilari crassna.<\/i> Pierre \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n t\u1eeb ch\u1ed3i ng\u1ecdn v\u00e0 ch\u1ed3i n\u00e1ch c\u1ee7a c\u00e2y in vitro<\/i>. S\u1ef1 nh\u00e2n ch\u1ed3i \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n t\u1eeb ch\u1ed3i ng\u1ecdn tr\u00ean m\u00f4i tr\u01b0\u1eddng MS ch\u1ee9a BA 0,2 mg\/l; Kinetin 0,2 mg\/l v\u00e0 Adenin 0,1 mg\/l. S\u1ef1 t\u0103ng tr\u01b0\u1edfng c\u1ee7a ng\u1ecdn ch\u1ed3i (ch\u1ee9a m\u00f4 ph\u00e2n sinh ng\u1ecdn hay ch\u1ed3i n\u00e1ch) x\u1ea3y ra tr\u00ean m\u00f4i tr\u01b0\u1eddng MS kh\u00f4ng hormon, t\u1eeb c\u00e1c \u0111o\u1ea1n c\u1eaft ch\u1ee9a 1 – 2 \u0111\u1ed1t (ch\u1ed3i n\u00e1ch \u1edf tr\u1ea1ng th\u00e1i ng\u1ee7). Vai tr\u00f2 c\u1ee7a c\u00e1c ch\u1ea5t \u0111i\u1ec1u ho\u00e0 sinh tr\u01b0\u1edfng th\u1ef1c v\u1eadt (Auxin, Cytokinin v\u00e0 Adenin). T\u1ef7 l\u1ec7 Auxin \/ Cytokinin v\u00e0 v\u1ecb tr\u00ed c\u1ee7a ch\u1ed3i n\u00e1ch tr\u00ean th\u00e2n s\u1ef1 ph\u00e1t tri\u1ec3n ch\u1ed3i \u0111\u01b0\u1ee3c th\u1ea3o lu\u1eadn. <\/p>\n

M\u1edf \u0111\u1ea7u<\/b> <\/p>\n

D\u00f3 tr\u1ea7m l\u00e0 m\u1ed9t trong nh\u1eefng lo\u00e0i c\u00e2y l\u00e2m s\u1ea3n ngo\u00e0i g\u1ed7 c\u00f3 gi\u00e1 tr\u1ecb. Trong nh\u1eefng n\u0103m g\u1ea7n \u0111\u00e2y, m\u1ed9t s\u1ed1 c\u00e1c doanh nghi\u1ec7p n\u01b0\u1edbc ngo\u00e0i l\u1eabn t\u01b0 nh\u00e2n v\u00e0 c\u00e1c h\u1ed9 gia \u0111\u00ecnh \u1edf c\u00e1c \u0111\u1ecba ph\u01b0\u01a1ng trong c\u1ea3 n\u01b0\u1edbc \u0111\u1ec1u \u0111\u00e3 b\u1ecf v\u1ed1n \u0111\u1ea7u t\u01b0 \u0111\u1ec3 g\u00e2y tr\u1ed3ng c\u00e2y D\u00f3 tr\u1ea7m th\u00e0nh c\u00e1c r\u1eebng tr\u1ed3ng t\u1eadp trung. V\u00ec th\u1ebf, nhu c\u1ea7u v\u1ec1 gi\u1ed1ng r\u1ea5t kh\u00f3 kh\u0103n v\u00ec ph\u1ea7n l\u1edbn di\u1ec7n t\u00edch tr\u1ed3ng c\u00e2y D\u00f3 tr\u1ea7m ch\u1ee7 y\u1ebfu l\u00e0 b\u1eb1ng c\u00e2y con gieo \u01b0\u01a1m t\u1eeb h\u1ea1t n\u00ean ch\u1ea5t l\u01b0\u1ee3ng ngu\u1ed3n gi\u1ed1ng ch\u01b0a \u0111\u01b0\u1ee3c t\u1ed1t. Trong nghi\u00ean c\u1ee9u n\u00e0y, ch\u00fang t\u00f4i t\u00ecm hi\u1ec3u kh\u1ea3 n\u0103ng t\u1ea1o ch\u1ed3i in vitro<\/i> t\u1eeb m\u00f4 ph\u00e2n sinh ng\u1ecdn v\u00e0 s\u1ef1 ph\u00e1t tri\u1ec3n ch\u1ed3i \u1edf c\u00e1c v\u1ecb tr\u00ed kh\u00e1c nhau c\u1ee7a ch\u1ed3i n\u00e1ch tr\u00ean th\u00e2n c\u00e2y D\u00f3 tr\u1ea7m nhi\u1ec1u n\u0103m tu\u1ed5i; \u0111\u1ed3ng th\u1eddi, b\u01b0\u1edbc \u0111\u1ea7u s\u01b0u t\u1eadp gi\u1ed1ng c\u00e2y D\u00f3 tr\u1ea7m trong \u0111i\u1ec1u ki\u1ec7n nu\u00f4i c\u1ea5y in vitro<\/i>. <\/p>\n

V\u1eadt li\u1ec7u<\/b> <\/p>\n

C\u00e1c ch\u1ed3i n\u00e1ch c\u1ee7a c\u00e2y D\u00f3 tr\u1ea7m (c\u00e2y 8 n\u0103m tu\u1ed5i) tr\u1ed3ng t\u1ea1i v\u01b0\u1eddn Trung t\u00e2m Nghi\u00ean c\u1ee9u L\u00e2m \u0111\u1eb7c s\u1ea3n v\u00e0 c\u00e1c \u0111o\u1ea1n ch\u1ed3i ng\u1ecdn c\u00e2y con 5 – 6 th\u00e1ng tu\u1ed5i \u0111\u1ec3 so s\u00e1nh. <\/p>\n

ph\u01b0\u01a1ng ph\u00e1p<\/b> <\/p>\n

\u0110\u01b0a ch\u1ed3i n\u00e1ch, ch\u1ed3i ng\u1ecdn c\u1ee7a c\u00e2y D\u00f3 tr\u1ea7m v\u00e0o s\u01b0u t\u1eadp in vitro<\/i><\/b>: C\u1eaft ch\u1ed3i n\u00e1ch \u0111\u01b0\u1ee3c tr\u00ean th\u00e2n c\u00e2y D\u00f3 tr\u1ea7m v\u00e0 c\u00e1c ch\u1ed3i ng\u1ecdn tr\u00ean c\u00e2y con, t\u00e1ch ri\u00eang ng\u1ecdn ch\u1ed3i n\u00e1ch \u0111\u1ec3 l\u1ea1i kh\u00fac c\u1eaft \u0111o\u1ea1n th\u00e2n ch\u1ed3i n\u00e1ch (ch\u00fa \u00fd: m\u1eabu c\u1ea5y th\u01b0\u1eddng g\u1ed3m kho\u1ea3ng 1 – 2 \u0111\u1ed1t); kh\u1eed tr\u00f9ng b\u1eb1ng HgCl2<\/sub> 1% v\u00e0 c\u00f4 l\u1eadp tr\u00ean m\u00f4i tr\u01b0\u1eddng 1\/2 MS (Murashige & Skoog 1962). S\u1ef1 ph\u00e1t tri\u1ec3n ch\u1ed3i \u0111\u01b0\u1ee3c quan s\u00e1t sau 15 ng\u00e0y nu\u00f4i c\u1ea5y \u1edf c\u00e1c \u0111i\u1ec1u ki\u1ec7n: \u00e1nh s\u00e1ng 2500 \u00b1 500 lux; nhi\u1ec7t \u0111\u1ed9 25 \u00b1 2 0<\/sup>C; \u1ea9m \u0111\u1ed9 55 \u00b1 5 %. Quan s\u00e1t v\u00e0 so s\u00e1nh s\u1ef1 kh\u00e1c nhau gi\u1eefa \u0111o\u1ea1n th\u00e2n ch\u1ed3i n\u00e1ch v\u00e0 c\u00e1c ch\u1ed3i ng\u1ecdn c\u1ee7a c\u00e2y con. <\/p>\n

Nu\u00f4i c\u1ea5y ch\u1ed3i in vitro: <\/i><\/b>C\u00e1c \u0111o\u1ea1n th\u00e2n ch\u1ed3i n\u00e1ch v\u00e0 c\u00e1c \u0111o\u1ea1n ch\u1ed3i ng\u1ecdn \u0111\u01b0\u1ee3c c\u1ea5y tr\u00ean m\u00f4i tr\u01b0\u1eddng MS v\u1edbi BA 0,2 mg\/l; Kinetin 0,2 mg\/l v\u00e0 Adenin 0,1 mg\/l. S\u1ef1 ph\u00e1t tri\u1ec3n c\u00e1c c\u1ee5m ch\u1ed3i \u0111\u01b0\u1ee3c quan s\u00e1t sau 40 – 45 ng\u00e0y nu\u00f4i c\u1ea5y, \u1edf c\u00e1c \u0111i\u1ec1u ki\u1ec7n: \u00e1nh s\u00e1ng 2500 \u00b1 500 lux; nhi\u1ec7t \u0111\u1ed9 25 \u00b1 2 0<\/sup>C; \u1ea9m \u0111\u1ed9 55 \u00b1 5 %. <\/p>\n

K\u1ebft qu\u1ea3<\/b> <\/p>\n

Kh\u1ea3 n\u0103ng t\u00e1i sinh ch\u1ed3i c\u1ee7a c\u00e1c m\u1eabu c\u1ea5y in vitro<\/i><\/b> <\/p>\n

Tr\u00ean m\u00f4i tr\u01b0\u1eddng 1\/2 MS, sau 15 ng\u00e0y nu\u00f4i c\u1ea5y, s\u1ef1 t\u00e1i sinh c\u1ee7a c\u00e1c m\u1eabu c\u1ea5y c\u00f3 s\u1ef1 kh\u00e1c bi\u1ec7t c\u1ea3 v\u1ec1 t\u1ef7 l\u1ec7 t\u00e1i sinh v\u00e0 ph\u1ea9m ch\u1ea5t c\u1ee7a ch\u1ed3i t\u00e1i sinh. C\u00e1c \u0111o\u1ea1n th\u00e2n c\u1ee7a ch\u1ed3i n\u00e1ch c\u00f3 kh\u1ea3 n\u0103ng t\u00e1i sinh m\u1ea1nh, t\u1ed1c \u0111\u1ed9 t\u00e1i sinh cao, ph\u1ea9m ch\u1ea5t c\u00e2y t\u1ed1t. (H\u00ecnh 1). <\/p>\n

\n\n\n\n\n\n\n
\n

\u0110o\u1ea1n m\u1eabu<\/b><\/p>\n<\/td>\n

\n

S\u1ed1<\/b> <\/p>\n

m\u1eabu<\/b><\/p>\n<\/td>\n

\n

T\u1ef7 l\u1ec7<\/b> <\/p>\n

t\u00e1i sinh<\/b><\/p>\n<\/td>\n

\n

T\u1ed1c \u0111\u1ed9<\/b> <\/p>\n

t\u00e1i sinh<\/b><\/p>\n<\/td>\n

\n

Ph\u1ea9m ch\u1ea5t<\/b><\/p>\n<\/td>\n<\/tr>\n

\n

\u0111o\u1ea1n th\u00e2n ch\u1ed3i n\u00e1ch<\/p>\n<\/td>\n

\n

20<\/p>\n<\/td>\n

\n

16\/20<\/p>\n<\/td>\n

\n

cao<\/p>\n<\/td>\n

\n

ch\u1ed3i kh\u1ecfe, m\u1eadp, l\u00e1 xanh \u0111\u1eadm<\/p>\n<\/td>\n<\/tr>\n

\n

ng\u1ecdn ch\u1ed3i n\u00e1ch<\/p>\n<\/td>\n

\n

20<\/p>\n<\/td>\n

\n

8\/20<\/p>\n<\/td>\n

\n

th\u1ea5p<\/p>\n<\/td>\n

\n

ch\u1ed3i y\u1ebfu<\/p>\n<\/td>\n<\/tr>\n

\n

ch\u1ed3i ng\u1ecdn<\/p>\n<\/td>\n

\n

20<\/p>\n<\/td>\n

\n

12\/20<\/p>\n<\/td>\n

\n

trung b\u00ecnh<\/p>\n<\/td>\n

\n

ch\u1ed3i non, g\u1ea7y, l\u00e1 xanh v\u00e0ng.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

S\u1ef1 nu\u00f4i c\u1ea5y Aquilari crassna. Pierre \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n t\u1eeb ch\u1ed3i ng\u1ecdn v\u00e0 ch\u1ed3i n\u00e1ch c\u1ee7a c\u00e2y in vitro. S\u1ef1 nh\u00e2n ch\u1ed3i \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n t\u1eeb ch\u1ed3i ng\u1ecdn tr\u00ean m\u00f4i tr\u01b0\u1eddng MS ch\u1ee9a BA 0,2 mg\/l; Kinetin 0,2 mg\/l v\u00e0 Adenin 0,1 mg\/l. S\u1ef1 t\u0103ng tr\u01b0\u1edfng c\u1ee7a ng\u1ecdn ch\u1ed3i (ch\u1ee9a m\u00f4 ph\u00e2n sinh ng\u1ecdn hay ch\u1ed3i n\u00e1ch) x\u1ea3y ra tr\u00ean m\u00f4i tr\u01b0\u1eddng MS kh\u00f4ng hormon, t\u1eeb c\u00e1c \u0111o\u1ea1n c\u1eaft ch\u1ee9a 1 – 2 \u0111\u1ed1t (ch\u1ed3i n\u00e1ch \u1edf tr\u1ea1ng th\u00e1i ng\u1ee7). Vai tr\u00f2 c\u1ee7a c\u00e1c ch\u1ea5t \u0111i\u1ec1u ho\u00e0 sinh tr\u01b0\u1edfng th\u1ef1c v\u1eadt (Auxin, Cytokinin v\u00e0 Adenin). T\u1ef7 l\u1ec7 Auxin \/ Cytokinin v\u00e0 v\u1ecb tr\u00ed c\u1ee7a ch\u1ed3i n\u00e1ch tr\u00ean th\u00e2n s\u1ef1 ph\u00e1t tri\u1ec3n ch\u1ed3i \u0111\u01b0\u1ee3c th\u1ea3o lu\u1eadn.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[13],"tags":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/8885"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=8885"}],"version-history":[{"count":1,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/8885\/revisions"}],"predecessor-version":[{"id":10341,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/8885\/revisions\/10341"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=8885"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/categories?post=8885"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/tags?post=8885"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}