{"id":463,"date":"2005-07-16T04:51:54","date_gmt":"2005-07-16T04:51:54","guid":{"rendered":"http:\/\/vafs.dungnq.local\/2005\/07\/16\/nhan-giong-mot-so-loai-cay-trong-rung-co-nang-suat-chat-luong-cao-bang-phuong-phap-nuoi-cay-mo\/"},"modified":"2019-09-24T11:17:07","modified_gmt":"2019-09-24T04:17:07","slug":"nhan-giong-mot-so-loai-cay-trong-rung-co-nang-suat-chat-luong-cao-bang-phuong-phap-nuoi-cay-mo","status":"publish","type":"post","link":"https:\/\/vafs.gov.vn\/vn\/nhan-giong-mot-so-loai-cay-trong-rung-co-nang-suat-chat-luong-cao-bang-phuong-phap-nuoi-cay-mo\/","title":{"rendered":"Nh\u00e2n gi\u1ed1ng m\u1ed9t s\u1ed1 lo\u00e0i c\u00e2y tr\u1ed3ng r\u1eebng c\u00f3 n\u0103ng su\u1ea5t, ch\u1ea5t l\u01b0\u1ee3ng cao b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y m\u00f4."},"content":{"rendered":"

\u0110o\u00e0n Th\u1ecb Mai, L\u00ea S\u01a1n, L\u01b0\u01a1ng Th\u1ecb Hoan, <\/i><\/b><\/p>\n

Ng\u00f4 Th\u1ecb Minh Duy\u00ean, Nguy\u1ec5n Thi\u00ean H\u01b0\u01a1ng<\/i><\/h2>\n

Vi\u1ec7n Khoa h\u1ecdc L\u00e2m nghi\u1ec7p Vi\u1ec7t Nam<\/i><\/h1>\n

Nu\u00f4i c\u1ea5y m\u00f4 l\u00e0 ph\u01b0\u01a1ng ph\u00e1p kh\u00e1 ph\u1ed5 bi\u1ebfn \u0111\u1ec3 nh\u00e2n nhanh c\u00e1c lo\u00e0i c\u00e2y tr\u1ed3ng ch\u1ea5t l\u01b0\u1ee3ng cao. Trong l\u00e2m nghi\u1ec7p ph\u01b0\u01a1ng ph\u00e1p n\u00e0y \u0111\u00e3 \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng t\u01b0\u01a1ng \u0111\u1ed1i s\u1edbm \u1edf m\u1ed9t s\u1ed1 n\u01b0\u1edbc ti\u00ean ti\u1ebfn nh\u01b0 Ph\u00e1p, \u0110\u1ee9c, Braxin, Trung Qu\u1ed1c… \u0111\u1ec3 nh\u00e2n nhanh m\u1ed9t s\u1ed1 gi\u1ed1ng c\u00f3 n\u0103ng su\u1ea5t cao.<\/p>\n

\u1edf Vi\u1ec7t Nam c\u00f4ng ngh\u1ec7 n\u00e0y \u0111\u00e3 \u0111\u01b0\u1ee3c du nh\u1eadp v\u00e0o c\u00f9ng v\u1edbi m\u1ed9t s\u1ed1 d\u00f2ng B\u1ea1ch \u0111\u00e0n t\u1eeb Trung Qu\u1ed1c v\u00e0o n\u0103m 1992 cho m\u1ed9t s\u1ed1 \u0111\u01a1n v\u1ecb l\u00e2m nghi\u1ec7p nh\u1eb1m nh\u00e2n nhanh c\u00e1c gi\u1ed1ng \u01b0u tr\u1ed9i ph\u1ee5c v\u1ee5 s\u1ea3n xu\u1ea5t.<\/p>\n

G\u1ea7n \u0111\u00e2y, Trung t\u00e2m Nghi\u00ean c\u1ee9u Gi\u1ed1ng c\u00e2y r\u1eebng thu\u1ed9c Vi\u1ec7n Khoa h\u1ecdc L\u00e2m nghi\u1ec7p Vi\u1ec7t Nam \u0111\u00e3 ch\u1ecdn t\u1ea1o \u0111\u01b0\u1ee3c m\u1ed9t s\u1ed1 gi\u1ed1ng c\u00f3 n\u0103ng su\u1ea5t v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng cao h\u01a1n h\u1eb3n c\u00e1c gi\u1ed1ng \u0111ang s\u1eed d\u1ee5ng trong s\u1ea3n xu\u1ea5t, trong \u0111\u00f3 c\u00f3 m\u1ed9t s\u1ed1 t\u1ed5 h\u1ee3p lai gi\u1eefa c\u00e1c lo\u00e0i Keo v\u00e0 B\u1ea1ch \u0111\u00e0n. Nh\u1eefng gi\u1ed1ng n\u00e0y \u0111\u00e1p \u1ee9ng \u0111\u01b0\u1ee3c y\u00eau c\u1ea7u c\u1ea5p b\u00e1ch v\u1ec1 tr\u1ed3ng r\u1eebng v\u00e0 s\u1ea3n xu\u1ea5t nguy\u00ean li\u1ec7u…<\/p>\n

Nu\u00f4i c\u1ea5y m\u00f4 l\u00e0 ph\u01b0\u01a1ng ph\u00e1p c\u00f3 kh\u1ea3 n\u0103ng nh\u00e2n nhanh m\u1ed9t s\u1ed1 l\u01b0\u1ee3ng l\u1edbn, \u0111\u1ea3m b\u1ea3o gi\u1eef \u0111\u01b0\u1ee3c c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m di truy\u1ec1n t\u1ed1t v\u00e0 gi\u1eef \u0111\u01b0\u1ee3c t\u00ednh tr\u1ebb ho\u00e1. \u0110\u00e2y l\u00e0 ph\u01b0\u01a1ng ph\u00e1p t\u1ed1t nh\u1ea5t \u0111\u1ec3 gi\u1ea3i quy\u1ebft nhu c\u1ea7u c\u00e2y con ph\u1ee5c v\u1ee5 tr\u1ed3ng r\u1eebng s\u1ea3n xu\u1ea5t tr\u00ean quy m\u00f4 l\u1edbn v\u1edbi \u0111\u1ed9 \u0111\u1ed3ng \u0111\u1ec1u cao, \u0111\u00e1p \u1ee9ng y\u00eau c\u1ea7u s\u1ea3n xu\u1ea5t c\u00f4ng nghi\u1ec7p, g\u00f3p ph\u1ea7n th\u1ef1c hi\u1ec7n c\u00f3 hi\u1ec7u qu\u1ea3 ch\u01b0\u01a1ng tr\u00ecnh tr\u1ed3ng m\u1edbi 5 tri\u1ec7u hecta r\u1eebng.<\/p>\n

1. \u0110\u1ed1i t\u01b0\u1ee3ng, v\u1eadt li\u1ec7u v\u00e0 ph\u01b0\u01a1ng ph\u00e1p nghi\u00ean c\u1ee9u.<\/b><\/p>\n

\u0110\u1ed1i t\u01b0\u1ee3ng.<\/i><\/p>\n

– Keo lai (A. mangium X A. auriculiformis<\/i>). : d\u00f2ng BV10<\/p>\n

– Keo l\u00e1 tr\u00e0m (Acaia auriculiformis<\/i>): d\u00f2ng 83.<\/p>\n

– B\u1ea1ch \u0111\u00e0n lai (E.urophyllaX E.camaldulesis<\/i>): t\u1ed5 h\u1ee3p lai U29<\/sub>C3 <\/sub><\/p>\n

V\u1eadt li\u1ec7u.<\/i><\/p>\n

– L\u00e0 c\u00e1c \u0111o\u1ea1n ch\u1ed3i g\u1ed1c d\u00e0i 10-15cm c\u1ee7a c\u00e2y con 6 th\u00e1ng \u0111\u1ebfn 1 n\u0103m tu\u1ed5i \u0111\u01b0\u1ee3c d\u1eabn gi\u1ed1ng t\u1eeb c\u00e1c c\u00e2y tr\u1ed9i \u0111\u00e3 qua ch\u1ecdn l\u1ecdc.<\/p>\n

 <\/p>\n

Kh\u1eed tr\u00f9ng b\u1ec1 m\u1eb7t.<\/i><\/p>\n

M\u1eabu v\u1eadt \u0111\u01b0\u1ee3c kh\u1eed tr\u00f9ng qua c\u00e1c b\u01b0\u1edbc sau:<\/p>\n

– R\u1eeda qua v\u00f2i n\u01b0\u1edbc ch\u1ea3y li\u00ean t\u1ee5c.<\/p>\n

– X\u1eed l\u00fd b\u1eb1ng c\u00e1c ch\u1ea5t t\u1ea9y nh\u1eb9.<\/p>\n

– Lau b\u1eb1ng c\u1ed3n 700<\/sup>.<\/p>\n

– Tr\u00e1ng b\u1eb1ng n\u01b0\u1edbc c\u1ea5t v\u00f4 tr\u00f9ng.<\/p>\n

– Kh\u1eed tr\u00f9ng b\u1eb1ng HgCl2 <\/sub>0.1%trong c\u00e1c th\u1eddi gian kh\u00e1c nhau.<\/p>\n

 <\/p>\n

M\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y.<\/i><\/p>\n

L\u00e0m\u00f4i tr\u01b0\u1eddng Murashige & Skoog c\u1ea3i ti\u1ebfn (MS*) c\u00f3 b\u1ed5 xung m\u1ed9t s\u1ed1 ch\u1ea5t \u0111i\u1ec1u ho\u00e0 sinh tr\u01b0\u1edfng thu\u1ed9c hai nh\u00f3m Auxin , Cytokinin v\u00e0 ch\u1ea5t ph\u1ee5 gia , c\u00e1c Vitamin \u0111\u01b0\u1eddng, A ga v\u1eeba \u0111\u1ee7.<\/p>\n

\u0110i\u1ec1u ch\u1ec9nh pH= 5.8 v\u1edbi Keo lai v\u00e0 Keo l\u00e1 tr\u00e0m, pH= 5.8-6.0 \u0111\u1ed1i v\u1edbi m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y B\u1ea1ch \u0111\u00e0n.<\/p>\n

Th\u1eddi gian h\u1ea5p kh\u1eed tr\u00f9ng 20 ph\u00fat (1.2 kg\/ cm3<\/sup> t\u1ea1i 1210<\/sup>C).<\/p>\n

\u0110i\u1ec1u ki\u1ec7n nu\u00f4i c\u1ea5y.<\/i><\/p>\n

Ch\u1ebf \u0111\u1ed9 \u00e1nh s\u00e1ng 3000lux chi\u1ebfu s\u00e1ng10 gi\u1edd 1 ng\u00e0y, nhi\u1ec7t \u0111\u1ed9 25 \u00b120<\/sup>C \u0111\u1ed1i v\u1edbi Keo lai v\u00e0 Keo l\u00e1 tr\u00e0m.<\/p>\n

Ch\u1ebf \u0111\u1ed9 \u00e1nh s\u00e1ng 2000lux chi\u1ebfu s\u00e1ng8 gi\u1edd 1 ng\u00e0y, nhi\u1ec7t \u0111\u1ed9 27 \u00b120<\/sup>C \u0111\u1ed1i v\u1edbi B\u1ea1ch \u0111\u00e0n<\/p>\n

C\u00e1c th\u00ed nghi\u1ec7m \u0111\u1ec1u \u0111\u01b0\u1ee3c b\u1ed1 tr\u00ed nhi\u1ec1u l\u1ea7n l\u1eb7p v\u00e0 x\u1eed l\u00fd k\u1ebft qu\u1ea3 theo ph\u01b0\u01a1ng ph\u00e1p th\u1ed1ng k\u00ea th\u00f4ng th\u01b0\u1eddng.<\/p>\n

2. K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u.<\/b><\/p>\n

Kh\u1eed tr\u00f9ng.<\/i><\/p>\n

C\u00e1c \u0111o\u1ea1n ch\u1ed3i \u0111\u01b0\u1ee3c x\u1eed l\u00fd b\u1eb1ng dung d\u1ecbch HgCl2<\/sub> 0,1 v\u1edbi th\u1eddi gian 2′, 4′, 6′,8′, 10′, 12′.(ph\u00fat)<\/p>\n

K\u1ebft qu\u1ea3 cho th\u1ea5y x\u1eed l\u00fd 12′ t\u1ef7 l\u1ec7 nhi\u1ec5m v\u00e0 t\u1ef7 l\u1ec7 b\u1eadt ch\u1ed3i c\u1ee7a Keo lai v\u00e0 B\u1ea1ch \u0111\u00e0n lai \u0111\u1ec1u b\u1eb1ng 0. \u0110i\u1ec1u \u0111\u00f3 ch\u1ee9ng t\u1ecf \u1edf th\u1eddi gian n\u00e0y HgCl\u00ad2<\/sub> \u0111\u00e3 th\u1ea5m s\u00e2u v\u00e0o t\u1ebf b\u00e0o ch\u1ea5t, ph\u00e1 hu\u1ef7 ch\u1ea5t nguy\u00ean sinh, \u1ea3nh h\u01b0\u1edfng t\u1edbi m\u1ecdi qu\u00e1 tr\u00ecnh trao \u0111\u1ed5i ch\u1ea5t c\u1ee7a t\u1ebf b\u00e0o<\/p>\n

X\u1eed l\u00fd HgCl2<\/sub> t\u1eeb 2- 4 ph\u00fat cho c\u1ea3 3 \u0111\u1ed1i t\u01b0\u1ee3ng tr\u00ean t\u1ef7 l\u1ec7 nhi\u1ec5m t\u0103ng ch\u1ee9ng t\u1ecf \u1edf th\u1eddi gian x\u1eed l\u00fd th\u1ea5p, ch\u01b0a \u0111\u1ee7 lo\u1ea1i tr\u1eeb m\u1ed9t s\u1ed1 n\u1ea5m b\u1ec7nh, b\u1ee5i b\u1ea9n b\u00e1m tr\u00ean m\u1eabu v\u1eadt.<\/p>\n

V\u1edbi B\u1ea1ch \u0111\u00e0n th\u1eddi gian x\u1eed l\u00fd t\u1eeb 6-8 ph\u00fat cho k\u1ebft qu\u1ea3 cao t\u1ef7 l\u1ec7 nhi\u1ec5m gi\u1ea3m h\u1eb3n ch\u1ec9 c\u00f2n 20-24%, t\u1ef7 l\u1ec7 b\u1eadt ch\u1ed3i \u0111\u1ea1t 38-60%. Ri\u00eang Keo lai v\u00e0 Keo l\u00e1 tr\u00e0m th\u1eddi gian x\u1eed l\u00fd t\u1eeb 8-10 ph\u00fat \u0111\u1ea1t hi\u1ec7u qu\u1ea3 cao nh\u1ea5t t\u1ef7 l\u1ec7 nhi\u1ec5m 48%, t\u1ef7 l\u1ec7 b\u1eadt ch\u1ed3i t\u0103ng \u0111\u1ea1t t\u1edbi 60%.<\/p>\n

Th\u1eddi gian thu m\u1eabu trong n\u0103m cho Keo lai v\u00e0 B\u1ea1ch \u0111\u00e0n v\u00e0o v\u1ee5 H\u00e8 -Thu th\u00edch h\u1ee3p nh\u1ea5t. Ri\u00eang v\u1edbi Keo l\u00e1 tr\u00e0m v\u1ee5 Xu\u00e2n-H\u00e8 l\u00e0 th\u00edch h\u1ee3p, v\u00ec \u1edf th\u1eddi gian tr\u00ean c\u00e2y \u00edt b\u1ecb n\u1ea5m b\u1ec7nh h\u01a1n.<\/p>\n

Nh\u00e2n ch\u1ed3i.<\/i><\/p>\n

Sau 10-15 ng\u00e0y c\u1ea5y, c\u00e1c ch\u1ed3i b\u1ea5t \u0111\u1ecbnh xu\u1ea5t hi\u1ec7n, khi chi\u1ec1u cao \u0111\u1ea1t 1,5-2cm ch\u1ed3i \u0111\u01b0\u1ee3c t\u00e1ch ra, c\u1ea5y v\u00e0o m\u00f4i tr\u01b0\u1eddng MS c\u1ea3i ti\u1ebfn (MS*) c\u00f3 b\u1ed5 xung: BAP (6-benzyl- amino purine), Kn (kinetin), \u1edf c\u00e1c n\u1ed3ng \u0111\u1ed9 kh\u00e1c nhau.<\/p>\n

K\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m cho th\u1ea5y khi d\u00f9ng ri\u00eang r\u1ebd th\u00ec BAP t\u1ecf ra l\u00e0 th\u00edch h\u1ee3p h\u01a1n c\u1ea3 trong vi\u1ec7c k\u00edch th\u00edch t\u1ea1o ch\u1ed3i cho c\u1ea3 3 \u0111\u1ed1i t\u01b0\u1ee3ng Keo lai, B\u1ea1ch \u0111\u00e0n lai v\u00e0 Keo l\u00e1 tr\u00e0m.<\/p>\n

M\u00f4i tr\u01b0\u1eddng MS* b\u1ed5 xung BAP n\u1ed3ng \u0111\u1ed9 0.5mg\/l th\u00edch h\u1ee3p nh\u1ea5t cho B\u1ea1ch \u0111\u00e0n s\u1ed1 ch\u1ed3i\/c\u1ee5m \u0111\u1ea1t trung b\u00ecnh 17 ch\u1ed3i\/ c\u1ee5m \u0111\u1eb7c bi\u1ec7t c\u00f3 th\u1ec3 t\u1edbi 30 ch\u1ed3i\/ c\u1ee5m. C\u00f2n v\u1edbi Kn ngay c\u1ea3 khi h\u1ec7 s\u1ed1 nh\u00e2n ch\u1ed3i cao nh\u1ea5t c\u0169ng ch\u1ec9 \u0111\u1ea1t 5.6 ch\u1ed3i\/c\u1ee5m.<\/p>\n

Trong khi \u0111\u00f3 Keo lai l\u1ea1i th\u00edch h\u1ee3p h\u01a1n v\u1edbi m\u00f4i tr\u01b0\u1eddng nh\u00e2n ch\u1ed3i MS* b\u1ed5 xung BAP n\u1ed3ng \u0111\u1ed9 2.0mg\/l cho h\u1ec7 s\u1ed1 nh\u00e2n ch\u1ed3i 23.3.<\/p>\n

Keo l\u00e1 tr\u00e0m th\u00edch h\u1ee3p h\u01a1n v\u1edbi m\u00f4i tr\u01b0\u1eddng MS* b\u1ed5 xung BAP n\u1ed3ng \u0111\u1ed9 1.0mg\/l h\u1ec7 s\u1ed1 nh\u00e2n ch\u1ed3i \u0111\u1ea1t 8.9.<\/p>\n

Ri\u00eang \u0111\u1ed1i v\u1edbi B\u1ea1ch \u0111\u00e0n h\u1ec7 s\u1ed1 nh\u00e2n ch\u1ed3i t\u0103ng kh\u00f4ng nh\u1eefng \u1ea3nh h\u01b0\u1edfng b\u1edfi c\u00e1c cytokinin \u0111\u01b0\u1ee3c b\u1ed5 xung v\u00e0o m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y m\u00e0 c\u00f2n ph\u1ee5 thu\u1ed9c r\u1ea5t nhi\u1ec1u v\u00e0o c\u00e1c y\u1ebfu t\u1ed1 kh\u00e1c nh\u01b0: ch\u1ebf \u0111\u1ed9 \u00e1nh s\u00e1ng v\u00e0 ph\u01b0\u01a1ng th\u1ee9c c\u1ea5y nh\u00e2n ch\u1ed3i. Th\u00ed nghi\u1ec7m cho th\u1ea5y ch\u1ebf \u0111\u1ed9 chi\u1ebfu s\u00e1ng \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh theo t\u1ef7 l\u1ec7 s\u00e1ng: t\u1ed1i: s\u00e1ng t\u01b0\u01a1ng \u1ee9ng: 1:2:2 cho k\u1ebft qu\u1ea3 t\u1ed1t nh\u1ea5t, \u1edf ch\u1ebf \u0111\u1ed9 n\u00e0y c\u00e2y ph\u00e1t tri\u1ec3n \u0111\u1ec1u, h\u1ec7 s\u1ed1 nh\u00e2n, s\u1ee9c s\u1ed1ng c\u1ee7a ch\u1ed3i t\u0103ng. \u0110\u1eb7c bi\u1ec7t khi \u0111i\u1ec1u ch\u1ec9nh ch\u1ebf \u0111\u1ed9 \u00e1nh s\u00e1ng theo t\u1ef7 l\u1ec7 n\u00e0y s\u1ebd r\u00fat ng\u1eafn th\u1eddi gian gi\u1eefa hai l\u1ea7n c\u1ea5y nh\u00e2n ch\u1ed3i t\u1eeb 20-25 ng\u00e0y\/ l\u1ea7n c\u00f2n 10-15 ng\u00e0y\/ l\u1ea7n c\u1ea5y chuy\u1ec3n. Trong khi \u0111\u00f3 Keo lai v\u00e0 Keo l\u00e1 tr\u00e0m h\u1ec7 s\u1ed1 nh\u00e2n ch\u1ed3i t\u0103ng kh\u00f4ng nh\u1eefng ch\u1ec9 \u1ea3nh h\u01b0\u1edfng b\u1edfi ch\u1ea5t \u0111i\u1ec1u ho\u00e0 sinh tr\u01b0\u1edfng m\u00e0 c\u00f2n ph\u1ee5 thu\u1ed9c r\u1ea5t nhi\u1ec1u v\u00e0o th\u00e0nh ph\u1ea7n c\u00e1c ch\u1ea5t ph\u1ee5 gia kh\u00e1c trong m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y, t\u1eeb \u0111\u00f3 r\u00fat ng\u1eafn \u0111\u01b0\u1ee3c th\u1eddi gian c\u1ea5y chuy\u1ec3n.<\/p>\n

Ra r\u1ec5.<\/i><\/p>\n

Khi ch\u1ed3i \u0111\u1ea1t chi\u1ec1u cao t\u1eeb 2.5-3 cm \u0111\u1ed1i v\u1edbi B\u1ea1ch \u0111\u00e0n, 3-4 cm \u0111\u1ed1i v\u1edbi Keo lai v\u00e0 Keo l\u00e1 tr\u00e0m, c\u00f3 th\u1ec3 c\u1eaft \u0111\u1ec3 c\u1ea5y chuy\u1ec3n sang m\u00f4i tr\u01b0\u1eddng h\u00ecnh th\u00e0nh r\u1ec5 l\u00e0 m\u00f4i tr\u01b0\u1eddng 1\/2MS* c\u00f3 b\u1ed5 xung IBA (axit b-indol butyric ), NAA ( axit a- naphtyl acetic).<\/p>\n

Keo l\u00e1 tr\u00e0m ra r\u1ec5 t\u1ed1t nh\u1ea5t trong m\u00f4i tr\u01b0\u1eddng MS* b\u1ed5 sung IBA \u1edf n\u1ed3ng \u0111\u1ed9 2.0mg\/l cho t\u1ef7 l\u1ec7 ra r\u1ec5 \u0111\u1ea1t 99.2% trong khi s\u1eed d\u1ee5ng NAA c\u00f9ng n\u1ed3ng \u0111\u1ed9 t\u1ef7 l\u1ec7 n\u00e0y c\u0169ng \u0111\u1ea1t 92%. Nh\u01b0 v\u1eady IBA v\u00e0 NAA \u0111\u1ec1u c\u00f3 t\u00e1c d\u1ee5ng m\u1ea1nh \u0111\u1ed1i v\u1edbi qu\u00e1 tr\u00ecnh ra r\u1ec5 c\u1ee7a Keo l\u00e1 tr\u00e0m.<\/p>\n

Trong khi \u0111\u00f3 v\u1edbi Keo lai, t\u1ef7 l\u1ec7 ra r\u1ec5 cao nh\u1ea5t \u0111\u1ea1t 98.3% khi d\u00f9ng m\u00f4i tr\u01b0\u1eddng MS* b\u1ed5 sung IBA n\u1ed3ng \u0111\u1ed9 2.0mg\/l nh\u01b0ng khi d\u00f9ng NAA c\u00f9ng n\u1ed3ng \u0111\u1ed9 t\u1ef7 l\u1ec7 ra r\u1ec5 ch\u1ec9 \u0111\u1ea1t cao nh\u1ea5t l\u00e0 85.6%.<\/p>\n

V\u1edbi B\u1ea1ch \u0111\u00e0n lai m\u00f4i tr\u01b0\u1eddng ra r\u1ec5 th\u00edch h\u1ee3p l\u00e0 m\u00f4i tr\u01b0\u1eddng MS* b\u1ed5 sung IBA n\u1ed3ng \u0111\u1ed9 1.5mg\/l t\u1ef7 l\u1ec7 ra r\u1ec5 c\u0169ng \u0111\u1ea1t 97.3% trong khi d\u00f9ng NAA t\u1ef7 l\u1ec7 ra r\u1ec5 cao nh\u1ea5t ch\u1ec9 \u0111\u1ea1t 56%, n\u1ebfu d\u00f9ng k\u1ebft h\u1ee3p IBA v\u00e0 NAA t\u1ef7 l\u1ec7 ra r\u1ec5 cao nh\u1ea5t ch\u1ec9 \u0111\u1ea1t 72%.<\/p>\n

Nh\u01b0 v\u1eady \u0111\u1ed1i v\u1edbi Keo lai v\u00e0 B\u1ea1ch \u0111\u00e0n, n\u1ebfu c\u00f9ng s\u1eed d\u1ee5ng n\u1ed3ng \u0111\u1ed9 nh\u01b0 nhau th\u00ec vi\u1ec7c b\u1ed5 sung th\u00eam IBA lu\u00f4n cho t\u1ec9 l\u1ec7 cao h\u01a1n so v\u1edbi b\u1ed5 sung NAA v\u00e0o m\u00f4i tr\u01b0\u1eddng c\u1ea5y. Do \u0111\u00f3 trong giai \u0111o\u1ea1nra r\u1ec5, invitro IBA c\u00f3 vai tr\u00f2 quan tr\u1ecdng, \u1edf giai \u0111o\u1ea1n n\u00e0y ch\u1ec9 c\u1ea7n b\u1ed5 sung IBA ri\u00eang r\u1ebd v\u00e0o m\u00f4i tr\u01b0\u1eddng t\u1ea1o r\u1ec5 m\u00e0 kh\u00f4ng c\u1ea7n s\u1ef1 c\u00f3 m\u1eb7t c\u00e1c ch\u1ea5t \u0111i\u1ec1u ho\u00e0 sinh tr\u01b0\u1edfng kh\u00e1c.<\/p>\n

\u1ea3nh 1: Ra r\u1ec5 tr\u1ef1c ti\u1ebfp keo lai b\u1eb1ng thu\u1ed1c b\u1ed9t TTG<\/i><\/p>\n

\u0110\u1ec3 gi\u1ea3m b\u1edbt th\u1eddi gian t\u1ea1o r\u1ec5 trong \u1ed1ng nghi\u1ec7m, t\u0103ng kh\u1ea3 n\u0103ng ch\u1ed1ng ch\u1ecbu c\u1ee7a c\u00e2y tr\u01b0\u1edbc khi \u0111\u01b0a ra m\u00f4i tr\u01b0\u1eddng t\u1ef1 nhi\u00ean, c\u00e1c ch\u1ed3i c\u00f3 chi\u1ec1u cao \u0111\u1ea1t t\u1eeb 3,5-4 cm \u0111\u01b0\u1ee3c c\u1eaft x\u1eed l\u00fd b\u1eb1ng dung d\u1ecbch Benlat n\u1ed3ng \u0111\u1ed9 0,3 %, ch\u1ea5m thu\u1ed1c b\u1ed9t TTG (do Trung t\u00e2m Gi\u1ed1ng pha ch\u1ebf), c\u1eafm tr\u1ef1c ti\u1ebfp v\u00e0o c\u00e1t \u1ea9m \u0111\u00e3 x\u1eed l\u00fd thu\u1ed1c t\u00edm 0.15%, \u0111\u01b0\u1ee3c ch\u0103m s\u00f3c nh\u01b0 ph\u01b0\u01a1ng ph\u00e1p gi\u00e2m hom. Sau 15 ng\u00e0y t\u1ef7 l\u1ec7 ra r\u1ec5 \u0111\u1ea1t 95%., c\u1ea5y c\u00e2y v\u00e0o b\u1ea7u \u0111\u1ea5t t\u1ef7 l\u1ec7 s\u1ed1ng \u0111\u1ea1t tr\u00ean 80%. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y s\u1ebd t\u1eadn d\u1ee5ng nh\u00e2n c\u00f4ng, ti\u1ebft ki\u1ec7m \u0111\u01b0\u1ee3c th\u1eddi gian v\u00e0 v\u1eadt t\u01b0 h\u00f3a ch\u1ea5t, h\u1ea1 gi\u00e1 th\u00e0nh s\u1ea3n ph\u1ea9m.<\/p>\n

Ra ng\u00f4i v\u00e0 ch\u0103m s\u00f3c c\u00e2y con.<\/i><\/p>\n

C\u00e2y m\u00f4 sau khi chi\u1ec1u d\u00e0i r\u1ec5 \u0111\u1ea1t 1.5-2.0cm \u1edf c\u1ea3 3 \u0111\u1ed1i t\u01b0\u1ee3ng \u0111\u01b0\u1ee3c \u0111\u01b0a ra ngo\u00e0i nh\u00e0 hu\u1ea5n luy\u1ec7n cho th\u00edch nghi d\u1ea7n v\u1edbi c\u00e1c \u0111i\u1ec1u ki\u1ec7n m\u00f4i tr\u01b0\u1eddng trong 7-10 ng\u00e0y, r\u1ed3i c\u1ea5y v\u00e0o gi\u00e1 th\u1ec3 theo 3 c\u00e1ch, k\u1ebft qu\u1ea3 b\u01b0\u1edbc \u0111\u1ea7u cho th\u1ea5y:<\/p>\n

– C\u1ea5y tr\u1ef1c ti\u1ebfp v\u00e0o b\u1ea7u \u0111\u1ea5t + ph\u00e2n c\u0103mp\u00f4t, t\u1ef7 l\u1ec7 s\u1ed1ng \u0111\u1ea1t tr\u00ean 90% cho c\u1ea3 3 \u0111\u1ed1i t\u01b0\u1ee3ng, c\u00e2y con ph\u00e1t tri\u1ec3n t\u1ed1t.<\/p>\n

– C\u1ea5y tr\u1ef1c ti\u1ebfp v\u00e0o b\u1ea7u \u0111\u1ea5t + ph\u00e2n NPK + x\u01a1 d\u1eeba t\u1ef7 l\u1ec7 s\u1ed1ng \u0111\u1ea1t tr\u00ean 85% cho c\u1ea3 3 \u0111\u1ed1i t\u01b0\u1ee3ng.<\/p>\n

– C\u1ea5y v\u00e0o b\u1ea7u \u0111\u1ea5t kh\u00f4ng tr\u1ed9n c\u00e1c th\u00e0nh ph\u1ea7n tr\u00ean, t\u1ef7 l\u1ec7 s\u1ed1ng th\u1ea5p h\u01a1n, cao nh\u1ea5t ch\u1ec9 \u0111\u1ea1t 67% v\u1edbi Keo lai v\u00e0 Keo l\u00e1 tr\u00e0m, ri\u00eang B\u1ea1ch \u0111\u00e0n ch\u1ec9 \u0111\u1ea1t 55%.<\/p>\n

3. K\u1ebft lu\u1eadn.<\/b><\/p>\n

– Ho\u00e1 ch\u1ea5t kh\u1eed tr\u00f9ng th\u00edch h\u1ee3p cho c\u00e1c \u0111\u1ed1i t\u01b0\u1ee3ng tr\u00ean l\u00e0 HgCl; v\u1edbi Keo lai v\u00e0 Keo l\u00e1 tr\u00e0m th\u1eddi gian kh\u1eed tr\u00f9ng l\u00e0 8-10 ph\u00fat, B\u1ea1ch \u0111\u00e0n l\u00e0 6-8 ph\u00fat. M\u1eabu v\u1eadt \u0111\u01b0\u1ee3c l\u1ea5y t\u1eeb c\u00e1c ch\u1ed3i g\u1ed1c cho t\u1ef7 l\u1ec7 t\u00e1i sinh ch\u1ed3i cao, kh\u1ea3 n\u0103ng ph\u00e1t tri\u1ec3n c\u1ee7a c\u00e2y t\u1ed1t h\u01a1n so v\u1edbi m\u1eabu v\u1eadt \u0111\u01b0\u1ee3c l\u1ea5y t\u1eeb c\u00e1c ch\u1ed3i c\u00e0nh.<\/p>\n

– M\u00f9a v\u1ee5 th\u00edch h\u1ee3p cho qu\u00e1 tr\u00ecnh kh\u1eed tr\u00f9ng v\u1edbi Keo lai v\u00e0 B\u1ea1ch \u0111\u00e0n l\u00e0 m\u00f9a H\u00e8-Thu, Keo l\u00e1 tr\u00e0m l\u00e0 v\u1ee5 Xu\u00e2n-H\u00e8. Th\u1eddi \u0111i\u1ec3m l\u1ea5y m\u1eabu th\u00edch h\u1ee3p trong tu\u1ea7n l\u00e0 th\u1ee9 ba \u0111\u1ebfn th\u1ee9 n\u0103m.<\/p>\n

+ B\u1ea1ch \u0111\u00e0n.<\/p>\n

– M\u00f4i tr\u01b0\u1eddng t\u1ea1o ch\u1ed3i th\u00edch h\u1ee3p l\u00e0 m\u00f4i tr\u01b0\u1eddng MS* c\u00f3 b\u1ed5 xung th\u00eam BAP n\u1ed3ng \u0111\u1ed9 0.5mg\/l, th\u1eddi gian c\u1ea5y chuy\u1ec3n th\u00edch h\u1ee3p l\u00e0 10 \u2013 12 ng\u00e0y\/ l\u1ea7n.<\/p>\n

– M\u00f4i tr\u01b0\u1eddng t\u1ea1o r\u1ec5 ph\u00f9 h\u1ee3p l\u00e0 m\u00f4i tr\u01b0\u1eddng MS* c\u00f3 b\u1ed5 xung IBA n\u1ed3ng \u0111\u1ed9 1.5mg\/ l.<\/p>\n

+ Keo l\u00e1 tr\u00e0m.<\/p>\n

– M\u00f4i tr\u01b0\u1eddng nh\u00e2n ch\u1ed3i l\u00e0 m\u00f4i tr\u01b0\u1eddng MS* b\u1ed5 sung BAP n\u1ed3ng \u0111\u1ed9 1.0mg\/l, th\u1eddi gian c\u1ea5y chuy\u1ec3n th\u00edch h\u1ee3p l\u00e0 20 \u2013 25 ng\u00e0y\/ l\u1ea7n.<\/p>\n

– M\u00f4i tr\u01b0\u1eddng ra r\u1ec5 th\u00edch h\u1ee3p l\u00e0 m\u00f4i tr\u01b0\u1eddng MS* c\u00f3 b\u1ed5 xung IBA n\u1ed3ng \u0111\u1ed9 2.0 mg\/ l.<\/p>\n

+ Keo lai.<\/p>\n

– M\u00f4i tr\u01b0\u1eddng nh\u00e2n ch\u1ed3i l\u00e0 m\u00f4i tr\u01b0\u1eddng MS* b\u1ed5 sung BAP n\u1ed3ng \u0111\u1ed9 2.0mg\/l, th\u1eddi gian c\u1ea5y chuy\u1ec3n th\u00edch h\u1ee3p l\u00e0 20 – 25 ng\u00e0y\/ l\u1ea7n.<\/p>\n

– M\u00f4i tr\u01b0\u1eddng ra r\u1ec5 th\u00edch h\u1ee3p l\u00e0 m\u00f4i tr\u01b0\u1eddng MS* c\u00f3 b\u1ed5 sung IBA n\u1ed3ng \u0111\u1ed9 2.0 mg\/ l.<\/p>\n

– Keo lai ra r\u1ec5 tr\u1ef1c ti\u1ebfp b\u1eb1ng thu\u1ed1c TTG cho t\u1ef7 l\u1ec7 ra r\u1ec5 cao. Tu\u1ef3 \u0111i\u1ec1u ki\u1ec7n c\u1ee5 th\u1ec3 m\u00e0 c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng ri\u00eang l\u1ebb ho\u1eb7c ph\u1ed1i h\u1ee3p hai ph\u01b0\u01a1ng ph\u00e1p ra r\u1ec5 tr\u00ean.<\/p>\n

T\u00e0i li\u1ec7u tham kh\u1ea3o<\/b><\/p>\n

1. L\u00ea \u0110\u00ecnh Kh\u1ea3, 1999, Nghi\u00ean c\u1ee9u s\u1eed d\u1ee5ng gi\u1ed1ng lai t\u1ef1 nhi\u00ean gi\u1eefa keo tai t\u01b0\u1ee3ng v\u00e0 keo l\u00e1 tr\u00e0m \u1edf Vi\u1ec7t Nam. Nh\u00e0 xu\u1ea5t b\u1ea3n N\u00f4ng nghi\u1ec7p.<\/p>\n

2. L\u00ea \u0110\u00ecnh Kh\u1ea3, \u0110o\u00e0n Th\u1ecb Mai, 2003. M\u1ed9t s\u1ed1 ph\u01b0\u01a1ng th\u1ee9c nh\u00e2n gi\u1ed1ng dinh d\u01b0\u1ee1ng trong s\u1ea3n xu\u1ea5t l\u00e2m nghi\u1ec7p. B\u1ed9 NN&PTNT, C\u00f4ng ngh\u1ec7 nh\u00e2n v\u00e0 s\u1ea3n xu\u1ea5t gi\u1ed1ng c\u00e2y tr\u1ed3ng, gi\u1ed1ng c\u00e2y l\u00e2m nghi\u1ec7p v\u00e0 gi\u1ed1ng v\u1eadt nu\u00f4i. Nh\u00e0 xu\u1ea5t b\u1ea3n Lao \u0111\u1ed9ng-x\u00e3 h\u1ed9i, trang 166-182.<\/p>\n

3. \u0110o\u00e0n Th\u1ecb Mai, Nguy\u1ec5n Vi\u1ec7t C\u01b0\u1eddng, Ng\u00f4 Th\u1ecb Minh Duy\u00ean, Nguy\u1ec5n Thi\u00ean Hu\u01a1ng, 2000. K\u1ebft qu\u1ea3 b\u01b0\u1edbc \u0111\u1ea7u v\u1ec1 nh\u00e2n gi\u1ed1ng b\u1ea1ch \u0111\u00e0n lai b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y m\u00f4 ph\u00e2n sinh. T\u1ea1p ch\u00ed L\u00e2m nghi\u1ec7p, s\u1ed1 10-2000, trang 46-47.<\/p>\n

4. \u0110o\u00e0n Th\u1ecb Mai, Ng\u00f4 Th\u1ecb Minh Duy\u00ean, L\u01b0\u01a1ng Th\u1ecb Hoan, 2001. K\u1ebft qu\u1ea3 nh\u00e2n gi\u1ed1ng m\u1ed9t s\u1ed1 d\u00f2ng keo lai b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y m\u00f4 ph\u00e2n sinh. T\u1ea1p ch\u00ed NN&PTNT, s\u1ed1 6-2001, trang 424-426.<\/p>\n

5. Nguy\u1ec5n Ng\u1ecdc T\u00e2n, Tr\u1ea7n H\u1ed3 Quang, 1997. Nh\u00e2n gi\u1ed1ng lai gi\u1eefa b\u1ea1ch \u0111\u00e0n li\u1ec5u v\u00e0 b\u1ea1ch \u0111\u00e0n tr\u1eafng b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p nu\u00f4i c\u1ea5y m\u00f4. K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u khoa h\u1ecdc v\u1ec1 ch\u1ecdn gi\u1ed1ng c\u00e2y r\u1eebng. Nh\u00e0 xu\u1ea5t b\u1ea3n N\u00f4ng nghi\u1ec7p, trang 103-107.<\/p>\n

Summary<\/b><\/p>\n

Acacia hybrid (clone BV10), A,auriculiformis (clone 83) and Ecalyotus hybrid (U29C3) are good germlasm that having high yield were chosen by Research Center for Forest Tree Improvement. In order to release it for forest plantaton, vegetative propagation by tissue culture is applied.<\/p>\n

Mercuric chloride (HgCl2<\/sub>) is used to sterilize the surface of the shoot. The best reason for shoots sterilization of A.auriculifomis<\/i>, Acacia and Eucalyptus hybrid are from spring to early summer and from summer to autumn, respectively.<\/p>\n

The best growth of Acacia hybrid and A. auriculiformis<\/i> responses were recorder with MS added BAP 2.0mg\/l media, MS with BAP 0.5 m\/l media seems to be optimum for shoot induction of Eucalyptus hybrid.<\/p>\n

The rooting medium for Acacia hybrid and A. auriculiformis<\/i> is modified MS media added IBA at concentration 2.0mg\/l, The best rooting for Eucalyptus hybrid responses were observed on modified MS + IBA 0.5 mg\/l media.The rooted plants were transferred to the training house maintained at 28 \u00b120 <\/sup>C for seven days. Thereafter, the plants were transferred to polybags containing equal quantities of soil, organic manure and other components. Depending on the weather conditions prevailing outside, the plant were kept inside polyhouse for two weeks and transferred to nursery. Following this procedure, more than 85% plants survived.<\/p>\n","protected":false},"excerpt":{"rendered":"

Nu\u00f4i c\u1ea5y m\u00f4 l\u00e0 ph\u01b0\u01a1ng ph\u00e1p kh\u00e1 ph\u1ed5 bi\u1ebfn \u0111\u1ec3 nh\u00e2n nhanh c\u00e1c lo\u00e0i c\u00e2y tr\u1ed3ng ch\u1ea5t l\u01b0\u1ee3ng cao. Trong l\u00e2m nghi\u1ec7p ph\u01b0\u01a1ng ph\u00e1p n\u00e0y \u0111\u00e3 \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng t\u01b0\u01a1ng \u0111\u1ed1i s\u1edbm \u1edf m\u1ed9t s\u1ed1 n\u01b0\u1edbc ti\u00ean ti\u1ebfn nh\u01b0 Ph\u00e1p, \u0110\u1ee9c, Braxin, Trung Qu\u1ed1c… \u0111\u1ec3 nh\u00e2n nhanh m\u1ed9t s\u1ed1 gi\u1ed1ng c\u00f3 n\u0103ng su\u1ea5t cao. <\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[18],"tags":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/463"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=463"}],"version-history":[{"count":3,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/463\/revisions"}],"predecessor-version":[{"id":19132,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/463\/revisions\/19132"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=463"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/categories?post=463"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/tags?post=463"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}