TH\u00d4NG TIN V\u1ec0 LU\u1eacN \u00c1N TI\u1ebeN S\u0128<\/p>\n
T\u00ean lu\u1eadn \u00e1n: Nghi\u00ean c\u1ee9u c\u01a1 s\u1edf khoa h\u1ecdc s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m vi sinh v\u1eadt \u0111a ch\u1ee7ng \u0111\u1ec3 gieo \u01b0\u01a1m v\u00e0 tr\u1ed3ng th\u00f4ng nh\u1ef1a (Pinus merkusii Jungh. Et de Vriese) tr\u00ean \u0111\u1ea5t tho\u00e1i ho\u00e1 \u1edf Mi\u1ec1n B\u1eafc Vi\u1ec7t Nam.
\nChuy\u00ean ng\u00e0nh: Qu\u1ea3n l\u00fd T\u00e0i nguy\u00ean r\u1eebng
\nM\u00e3 s\u1ed1: 62 62 02 11
\nH\u1ecd v\u00e0 t\u00ean NCS: Nguy\u1ec5n Th\u1ecb Thu\u00fd Nga
\nH\u1ecd v\u00e0 t\u00ean c\u00e1n b\u1ed9 h\u01b0\u1edbng d\u1eabn: PGS. TS. Ph\u1ea1m Quang Thu
\nC\u01a1 s\u1edf \u0111\u00e0o t\u1ea1o: Vi\u1ec7n Khoa h\u1ecdc L\u00e2m nghi\u1ec7p Vi\u1ec7t Nam
\nT\u00f3m t\u1eaft nh\u1eefng k\u1ebft lu\u1eadn m\u1edbi c\u1ee7a lu\u1eadn \u00e1n:
\n– Tuy\u1ec3n ch\u1ecdn ch\u1ee7ng Pt1 thu\u1ed9c lo\u00e0i n\u1ea5m Pisolithus tinctorius c\u1ed9ng sinh Th\u00f4ng nh\u1ef1a cho chi\u1ec1u cao c\u00e2y Th\u00f4ng nh\u1ef1a 4.7 cm t\u0103ng 55% so v\u1edbi \u0111\u1ed1i ch\u1ee9ng. L\u1ea7n \u0111\u1ea7u ti\u00ean ph\u00e2n l\u1eadp v\u00e0 tuy\u1ec3n ch\u1ecdn \u0111\u01b0\u1ee3c ch\u1ee7ng QI1 l\u00e0 lo\u00e0i Pseudomonas fluorescens sinh v\u1eeba c\u00f3 kh\u1ea3 n\u0103ng t\u1ed5ng h\u1ee3p IAA \u0111\u1ea1t 11,872mg\/l v\u00e0 \u0111\u1ed1i kh\u00e1ng n\u1ea5m Fusarium oxysporum g\u00e2y b\u1ec7nh th\u1ed1i c\u1ed5 r\u1ec5 c\u00e2y th\u00f4ng v\u1edbi \u0111\u01b0\u1eddng k\u00ednh v\u00f2ng \u1ee9c ch\u1ebf l\u00e0 20,1 mm. Nh\u00e2n sinh kh\u1ed1i ch\u1ee7ng QI1 \u0111i\u1ec1u ki\u1ec7n t\u1ed1i \u01b0u l\u00e0 m\u00f4i tr\u01b0\u1eddng g\u1ec9 \u0111\u01b0\u1eddng, th\u1eddi gian 120 gi\u1edd (5 ng\u00e0y), nhi\u1ec7t \u0111\u1ed9 250C, \u0111\u1ed9 pH th\u00edch h\u1ee3p 7- 7,5. L\u1ea7n \u0111\u1ea7u ti\u00ean ph\u00e2n l\u1eadp v\u00e0 tuy\u1ec3n ch\u1ecdn \u0111\u01b0\u1ee3c ch\u1ee7ng QI24 \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 lo\u00e0i Bacillus subtilis v\u1eeba c\u00f3 kh\u1ea3 n\u0103ng \u0111\u1ed1i kh\u00e1ng n\u1ea5m F. oxysporum g\u00e2y b\u1ec7nh th\u1ed1i c\u1ed5 r\u1ec5 th\u00f4ng, \u0111\u01b0\u1eddng k\u00ednh v\u00f2ng \u1ee9c ch\u1ebf l\u00e0 22 mm, v\u1eeba c\u00f3 kh\u1ea3 n\u0103ng sinh t\u1ed5ng h\u1ee3p IAA \u0111\u1ea1t 5,312mg\/l. Nh\u00e2n sinh kh\u1ed1i ch\u1ee7ng QI24 v\u1edbi \u0111i\u1ec1u ki\u1ec7n t\u1ed1i \u01b0u l\u00e0 m\u00f4i tr\u01b0\u1eddng PD, th\u1eddi gian 72 gi\u1edd (3 ng\u00e0y), nhi\u1ec7t \u0111\u1ed9 270C, \u0111\u1ed9 pH th\u00edch h\u1ee3p 7. L\u1ea7n \u0111\u1ea7u ti\u00ean ph\u00e2n l\u1eadp v\u00e0 tuy\u1ec3n ch\u1ecdn \u0111\u01b0\u1ee3c ch\u1ee7ng N2.1 l\u00e0 lo\u00e0i Burkholderia cenocepacia v\u1eeba c\u00f3 kh\u1ea3 n\u0103ng ph\u00e2n gi\u1ea3i ph\u1ed1t ph\u00e1t kh\u00f3 tan, \u0111\u01b0\u1eddng k\u00ednh v\u00f2ng ph\u00e2n gi\u1ea3i l\u00e0 23,5mm, sinh n\u1ed3ng \u0111\u1ed9 l\u00e2n d\u1ec5 ti\u00eau l\u00e0 420 ppm, v\u1eeba c\u00f3 kh\u1ea3 n\u0103ng c\u1ed1 \u0111\u1ecbnh nit\u01a1 \u0111\u1ea1t 2,12 mg\/ml NH4+. Nh\u00e2n sinh kh\u1ed1i ch\u1ee7ng N2.1 v\u1edbi \u0111i\u1ec1u ki\u1ec7n t\u1ed1i \u01b0u l\u00e0 m\u00f4i tr\u01b0\u1eddng Pikoskaya, th\u1eddi gian 72 gi\u1edd (3 ng\u00e0y), nhi\u1ec7t \u0111\u1ed9 270C- 300C, \u0111\u1ed9 pH th\u00edch h\u1ee3p 6,5. L\u1ea7n \u0111\u1ea7u ti\u00ean ph\u00e2n l\u1eadp v\u00e0 tuy\u1ec3n ch\u1ecdn \u0111\u01b0\u1ee3c ch\u1ee7ng V4.2 l\u00e0 lo\u00e0i Azotobacter beijerinskii v\u1eeba c\u00f3 kh\u1ea3 n\u0103ng c\u1ed1 \u0111\u1ecbnh nit\u01a1 \u0111\u1ea1t 4,18 mg\/ml NH4+, v\u1eeba c\u00f3 kh\u1ea3 n\u0103ng ph\u00e2n gi\u1ea3i ph\u1ed1t ph\u00e1t kh\u00f3 tan, \u0111\u01b0\u1eddng k\u00ednh v\u00f2ng ph\u00e2n gi\u1ea3i l\u00e0 17,2mm, sinh n\u1ed3ng \u0111\u1ed9 l\u00e2n d\u1ec5 ti\u00eau 271,32 ppm, Nh\u00e2n sinh kh\u1ed1i ch\u1ee7ng V4.2 v\u1edbi \u0111i\u1ec1u ki\u1ec7n t\u1ed1i \u01b0u l\u00e0 m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y NFMN, th\u1eddi gian 96 gi\u1edd (4 ng\u00e0y), nhi\u1ec7t \u0111\u1ed9 250C- 270C \u0111\u1ed9 pH th\u00edch h\u1ee3p 6,5 \u2013 7.
\n– C\u00f4ng th\u1ee9c t\u1ea1o ch\u1ebf ph\u1ea9m vi sinh v\u1eadt \u0111a ch\u1ee7ng d\u00f9ng cho c\u00e2y Th\u00f4ng nh\u1ef1a \u1edf r\u1eebng tr\u1ed3ng ch\u1ea5t l\u01b0\u1ee3ng t\u1ed1t nh\u1ea5t l\u00e0: 10kg nguy\u00ean li\u1ec7u trong \u0111\u00f3 35% b\u1ed9t apatit, 35% m\u00f9n, 10% Potassium polyacrylamide v\u00e0 20% sinh kh\u1ed1i vi sinh v\u1eadt c\u00e1c\u00a0 lo\u1ea1i. C\u00f4ng th\u1ee9c t\u1ea1o ch\u1ebf ph\u1ea9m vi sinh v\u1eadt \u0111a ch\u1ee7ng d\u00f9ng cho c\u00e2y Th\u00f4ng nh\u1ef1a \u1edf v\u01b0\u1eddn \u01b0\u01a1m ch\u1ea5t l\u01b0\u1ee3ng t\u1ed1t nh\u1ea5t l\u00e0: 10kg nguy\u00ean li\u1ec7u trong \u0111\u00f3 40% b\u1ed9t apatit, 40% m\u00f9n, v\u00e0 20% sinh kh\u1ed1i vi sinh v\u1eadt c\u00e1c\u00a0 lo\u1ea1i. Ch\u1ebf ph\u1ea9m vi sinh v\u1eadt \u0111a ch\u1ee7ng \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng, th\u1eddi gian s\u1eed d\u1ee5ng tr\u01b0\u1edbc 6 th\u00e1ng k\u1ec3 t\u1eeb ng\u00e0y s\u1ea3n xu\u1ea5t.
\n– B\u00f3n 2 gam ch\u1ebf ph\u1ea9m vi sinh v\u1eadt \u0111a ch\u1ee7ng \u0111\u1ed1i v\u1edbi c\u00e2y Th\u00f4ng nh\u1ef1a t\u1ea1i v\u01b0\u1eddn \u01b0\u01a1m, cho chi\u1ec1u cao \u0111\u1ea1t 22,54 cm (t\u0103ng h\u01a1n 23% so v\u1edbi \u0111\u1ed1i ch\u1ee9ng v\u00e0 t\u0103ng h\u01a1n c\u00e1c c\u00f4ng th\u1ee9c b\u00f3n vi sinh m\u1eadt \u0111\u1ed9 kh\u00e1c t\u1eeb 15 \u2013 18%), \u0111\u01b0\u1eddng k\u00ednh\u00a0 \u0111\u1ea1t 4,12 mm (t\u0103ng 28% so v\u1edbi \u0111\u1ed1i ch\u1ee9ng kh\u00f4ng b\u00f3n g\u00ec, t\u0103ng 24% so v\u1edbi c\u00f4ng th\u1ee9c \u0111\u00f3ng b\u1ea7u th\u00f4ng th\u01b0\u1eddng c\u00f3 1% l\u00e2n), t\u1ef7 l\u1ec7 b\u1ecb b\u1ec7nh gi\u1ea3m ch\u1ec9 c\u00f2n 3-5%. B\u00f3n 40 g ch\u1ebf ph\u1ea9m VSV \u0111a ch\u1ee7ng, chi\u1ec1u cao c\u00e2y Th\u00f4ng nh\u1ef1a \u1edf r\u1eebng tr\u1ed3ng, \u0111\u1ea1t 34,62 cm (t\u0103ng 26% so v\u1edbi \u0111\u1ed1i ch\u1ee9ng kh\u00f4ng b\u00f3n g\u00ec, t\u0103ng 19% so v\u1edbi \u0111\u1ed1i ch\u1ee9ng b\u00f3n ph\u00e2n NPK 20.20.15), \u0111\u01b0\u1eddng k\u00ednh g\u1ed1c c\u1ee7a c\u00e2y Th\u00f4ng nh\u1ef1a sau 1.5 tu\u1ed5i \u0111\u1ea1t 1,5 cm (t\u0103ng 44% so v\u1edbi \u0111\u1ed1i ch\u1ee9ng kh\u00f4ng b\u00f3n g\u00ec, t\u0103ng 27% so v\u1edbi \u0111\u1ed1i ch\u1ee9ng b\u00f3n ph\u00e2n NPK 20.20.15). S\u1eed d\u1ee5ng ch\u1ebf ph\u1ea9m \u0111a ch\u1ee7ng vi sinh v\u1eadt trong tr\u1ed3ng r\u1eebng \u0111\u00e3 c\u1ea3i thi\u1ec7n \u0111\u1ea5t tho\u00e1i ho\u00e1 b\u1ea1c m\u00e0u l\u01b0\u1ee3ng m\u00f9n t\u0103ng 1,2 l\u1ea7n, h\u00e0m l\u01b0\u1ee3ng l\u00e2n t\u1ed5ng s\u1ed1 t\u0103ng 1,3 c\u00e1c ch\u1ec9 ti\u00eau v\u1ec1 th\u00e0nh ph\u1ea7n v\u00e0 m\u1eadt \u0111\u1ed9 vi sinh v\u1eadt \u0111\u1ec1u t\u0103ng l\u00ean r\u00f5 d\u1ec7t v\u00e0 kh\u00f4ng th\u1ea5y xu\u1ea5t hi\u1ec7n n\u1ea5m Fusarium oxysporum\u00a0 g\u00e2y b\u1ec7nh th\u1ed1i c\u1ed5 r\u1ec5 c\u00e2y Th\u00f4ng nh\u1ef1a.<\/p>\n
INFORMATION OF DOCTORAL THESIS<\/p>\n
Thesis title: Study on scientific basis to produce multi-strain microorganism preparations for sowing and planting Pinus merkusii Jungh. Et de Vriese in degraded land in the North of Vietnam.
\nSpecialty: Forest resources management
\nCode: 62 62 02 11
\nPostgraduate student: Nguyen Thi Thuy Nga
\nSupervisor: Associate Professor Pham Quang Thu
\nEducation institution: Vietnamese Academy of Forest Sciences
\nKey results:
\n– Strain Pt1 of symbiotic fungi Pisolithus tinctorius was selected which can help increase the height of Pinus merkusii seedlings to 4.7 cm, a 55 percent rise compared to control. For the first time, strain QI1 (Pseudomonas fluorescens) which can synthesis IAA at 11.872 mg\/l\u00a0 and inhibit Fusarium oxysporum causing damping off in pine with the diameter of inhibit ring of 20.1 mm. The best growing condition of QI1 is molasses culture medium, 120-hour (5-day) growing time, temperature of 25oC and pH\u00a0 of 7 \u2013 7.5. Also, it is the first time that strain QI24 (Bacillus subtilis) isolated and selected. This strain can inhibit F. oxysporum causing damping off in pine with the diameter of inhibit ring of 22 mm and synthesis IAA at 5.312 mg\/L. The best growing condition of QI24 is\u00a0 PD culture medium, 72-hour (3-day) growing time, optimum temperature of 27oC, optimum pH of 7. Strain N2.1 (Burkholderia cenocepacia) which can dissolve insoluble phosphate with the diameter of dissolve ring of 23.5 mm, synthesis phosphorus with concentration of 420 ppm, nitrogen fixation ability of 2.12 mg\/ml NH4+. The best growing condition of N2.1 is Pikoskaya culture medium, 72-hour (3-day) growing time, optimum temperature of 27-30oC, optimum pH of 6.5 was isolated and selected. Strain V4.2 (Azotobacter beijerinskii) which can fix nitrogen by 4.18 mg\/mL NH4+, dissolve insoluble phosphate with the diameter of dissolve ring of 17.2mm and synthesis phosphorus with concentration of 271.32 ppm. The best growing condition of V4.2 is NFMN culture medium, 96-hour (4-day) growing time, optimum temperature of 25-27oC, optimum pH of 6.5-7, was also isolated and selected for the first time.
\n– Best formula of multi-strain microorganism preparation for nursery:10 kg including 35% apatite powder + 35% humus + 20% microorganisms + 10% potassium polyacrylamide. Best formula of multi-strain microorganism preparation for plantation: 10 kg including 40% apatite powder + 40% humus + 20% microorganisms. Such preparations can be stored at room temperature, used best before 6 months since produced.
\n– By applying 2 gr of the multi-strain microorganism preparations for Pinus merkusii in nursery,\u00a0 tree height reached 22.54 cm (23% increase compared to control and 15-18% increased compared to other preparation), diameter reached 4.12mm (28% increase compared to control, 24% increase compared to using normal container with 1% phosphate, disease incedence decreased to 3-5%. In plantation, tree height reached 34.62 cm (26% increase compared to control and 19% increase compared to using NPK 20.20.15), diameter of 1.5 year old trees reached 1.5 cm (44% increase compared to control, 27% increased compared to using NPK 20.20.15) as applying 40g multi-strain microorganism preparations for Pinus merkusii. Using the multi-strain microorganism preparations in plantation improved soil degradation, humus rose 1.2 times, total phosphates rose 1.3, composition and density of microorganisms increased considerably and Fusarium oxysporum that causes damping off disease in Pinus merkusii was not found.<\/p>\n
Chi ti\u1ebft lu\u1eadn \u00e1n v\u00e0 t\u00f3m t\u1eaft xem t\u1ea1i \u0111\u00e2y:\u00a0T\u00f3m t\u1eaft lu\u1eadn \u00e1n<\/a>\u00a0v\u00e0\u00a0Lu\u1eadn \u00e1n<\/a><\/p>\n \n","protected":false},"excerpt":{"rendered":" TH\u00d4NG TIN V\u1ec0 LU\u1eacN \u00c1N TI\u1ebeN S\u0128 T\u00ean lu\u1eadn \u00e1n: Nghi\u00ean c\u1ee9u c\u01a1 s\u1edf khoa h\u1ecdc s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m vi sinh v\u1eadt \u0111a ch\u1ee7ng \u0111\u1ec3 gieo \u01b0\u01a1m v\u00e0 tr\u1ed3ng th\u00f4ng nh\u1ef1a (Pinus merkusii Jungh. Et de Vriese) tr\u00ean \u0111\u1ea5t tho\u00e1i ho\u00e1 \u1edf Mi\u1ec1n B\u1eafc Vi\u1ec7t Nam. Chuy\u00ean ng\u00e0nh: Qu\u1ea3n l\u00fd T\u00e0i nguy\u00ean r\u1eebng M\u00e3 […]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[42,66],"tags":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/16228"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=16228"}],"version-history":[{"count":4,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/16228\/revisions"}],"predecessor-version":[{"id":17185,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/16228\/revisions\/17185"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=16228"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/categories?post=16228"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/tags?post=16228"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}