TH\u00d4NG TIN LU\u1eacN \u00c1N TI\u1ebeN S\u0128<\/p>\n
T\u00ean \u0111\u1ec1 t\u00e0i lu\u1eadn \u00e1n: \u201cNghi\u00ean c\u1ee9u ch\u1ecdn gi\u1ed1ng v\u00e0 nh\u00e2n gi\u1ed1ng tr\u00e0m c\u00f3 h\u00e0m l\u01b0\u1ee3ng tinh d\u1ea7u v\u00e0 t\u1ef7 l\u1ec7 1,8-cineole cao\u201d.
\nChuy\u00ean ng\u00e0nh: Di truy\u1ec1n v\u00e0 Ch\u1ecdn gi\u1ed1ng c\u00e2y l\u00e2m nghi\u1ec7p\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 M\u00e3 s\u1ed1: 62 62 0207
\nH\u1ecd v\u00e0 t\u00ean NCS: Khu\u1ea5t Th\u1ecb H\u1ea3i Ninh
\nH\u1ecd v\u00e0 t\u00ean c\u00e1n b\u1ed9 h\u01b0\u1edbng d\u1eabn: GS.TS.L\u00ea \u0110\u00ecnh Kh\u1ea3 v\u00e0 TS. Ph\u00ed H\u1ed3ng H\u1ea3i
\nT\u00ean c\u01a1 s\u1edf \u0111\u00e0o t\u1ea1o: Vi\u1ec7n Khao h\u1ecdc L\u00e2m nghi\u1ec7p Vi\u1ec7t Nam<\/p>\n
T\u00d3M T\u1eaeT NH\u1eeeNG K\u1ebeT LU\u1eacN M\u1edaI C\u1ee6A LU\u1eacN \u00c1N
\n1. Ch\u1ecdn gi\u1ed1ng
\n\u0110\u00e1nh gi\u00e1 t\u1ed5ng h\u1ee3p c\u00e1c t\u00ednh tr\u1ea1ng sinh tr\u01b0\u1edfng, h\u00e0m l\u01b0\u1ee3ng v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng tinh d\u1ea7u c\u1ee7a m\u1ed9t s\u1ed1 xu\u1ea5t x\u1ee9 v\u00e0 d\u00f2ng v\u00f4 t\u00ednh cho th\u1ea5y Tr\u00e0m n\u0103m g\u00e2n bi\u1ebfn d\u1ecb kh\u00e1 l\u1edbn v\u1ec1 sinh tr\u01b0\u1edfng, h\u00e0m l\u01b0\u1ee3ng v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng tinh d\u1ea7u. \u0110\u00e3 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c c\u00e1c xu\u1ea5t x\u1ee9 West Malam PNG (Q15), Wasua Road PNG (Q16), Casino NSW (Q23) v\u00e0 Gympie Qld (Q4), c\u00f9ng c\u00e1c d\u00f2ng v\u00f4 t\u00ednh Q4.40 Q4.45, Q15.013, Q15.32 v\u00e0 Q15.38 c\u00f3 h\u00e0m l\u01b0\u1ee3ng tinh d\u1ea7u>1,4% v\u00e0 t\u1ef7 l\u1ec7 1,8-cineole > 65%).
\n2. Nh\u00e2n gi\u1ed1ng b\u1eb1ng nu\u00f4i c\u1ea5y m\u00f4.
\nNh\u00e2n gi\u1ed1ng Tr\u00e0m n\u0103m g\u00e2n b\u1eb1ng nu\u00f4i c\u1ea5y m\u00f4 \u0111\u1ea1t k\u1ebft qu\u1ea3 t\u1ed1t nh\u1ea5t khi kh\u1eed tr\u00f9ng ch\u1ed3i n\u1eeda h\u00f3a g\u1ed7 b\u1eb1ng HgCl2 0,1% trong 6 ph\u00fat chia th\u00e0nh 2 l\u1ea7n (l\u1ea7n \u0111\u1ea7u 4 ph\u00fat v\u00e0 l\u1ea7n sau 2 ph\u00fat); sau \u0111\u00f3 t\u00e1i sinh ch\u1ed3i l\u1ea7n m\u1ed9t trong m\u00f4i tr\u01b0\u1eddng MS*( Nit\u01a1 = 1\/2 MS) b\u1ed5 sung 0,5 mg\/l BAP + 0,2 mg\/l Kinetin + 30 g\/l \u0111\u01b0\u1eddng sucrose; t\u00e1i sinh ch\u1ed3i l\u1ea7n hai l\u00e0 MS* b\u1ed5 sung 0,5 mg\/l BAP + 0,2mg\/l Kinetin + 30 g\/l glucose (ho\u1eb7c 20 g\/l glucose + 10 g\/l sucrose); t\u1ea1o c\u1ee5m ch\u1ed3i l\u00e0 MS* b\u1ed5 sung 0,2 – 0,5 mg\/l BAP + 0,2 mg\/l K + 0,1 mg\/l NAA; cho ra r\u1ec5 in vitro l\u00e0 MS* b\u1ed5 sung 0,3 – 0,5 mg\/l NAA + 30g\/l \u0111\u01b0\u1eddng sucrose (ho\u1eb7c 20 g\/l glucose + 10 g\/l sucrose). Theo ph\u01b0\u01a1ng ph\u00e1p n\u00e0y th\u00ec t\u1ef7 l\u1ec7 m\u1eabu b\u1ecb nhi\u1ec5m 3,3%, trong khi t\u1ef7 l\u1ec7 m\u00e2\u0303u sa\u0323ch l\u00e0 70 – 83,3%, t\u1ef7 l\u1ec7 m\u1eabu t\u00e1i sinh ch\u1ed3i 93,3 – 100% v\u1edbi 3,5 – 4 ch\u1ed3i\/n\u00e1ch l\u00e1; t\u1ef7 l\u1ec7 ra r\u1ec5 >90%, r\u1ec5 c\u00f3 ch\u1ea5t l\u01b0\u1ee3ng t\u1ed1t. Hu\u00e2\u0301n luy\u00ea\u0323n c\u00e2y m\u00f4 d\u01b0\u01a1\u0301i a\u0301nh sa\u0301ng ta\u0301n xa\u0323 trong 2 tu\u00e2\u0300n tr\u01b0\u01a1\u0301c khi c\u1ea5y v\u00e0o b\u1ea7u \u0111\u1ea5t \u1edf v\u01b0\u01a1\u0300n \u01b0\u01a1m th\u00ec c\u00e2y m\u00f4 c\u00f3 t\u1ef7 l\u1ec7 s\u00f4\u0301ng 86,7 – 91,1%.
\n3. \u1ea2nh h\u01b0\u1edfng c\u1ee7a ph\u00e2n b\u00f3n, chi\u1ec1u cao g\u1ed1c ch\u1eb7t v\u00e0 m\u00f9a v\u1ee5 \u0111\u1ebfn n\u0103ng su\u1ea5t v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng\u00a0 tinh d\u1ea7u
\nTh\u00ed nghi\u1ec7m b\u00f3n th\u00fac Tr\u00e0m n\u0103m g\u00e2n sau m\u1ed9t n\u0103m cho th\u1ea5y c\u00f4ng th\u1ee9c 100 g NPK+200 g ph\u00e2n h\u1eefu c\u01a1 vi sinh +50 g v\u00f4i b\u1ed9t\/c\u00e2y t\u1ed1t nh\u1ea5t \u0111\u1ed1i v\u1edbi xu\u1ea5t x\u1ee9 West Malam PNG (l\u01b0\u1ee3ng tinh d\u1ea7u\/c\u00e2y b\u1eb1ng 2,7 l\u1ea7n so v\u1edbi \u0111\u1ed1i ch\u1ee9ng), c\u00f4ng th\u1ee9c 200 g NPK+200 g ph\u00e2n h\u1eefu c\u01a1 vi sinh + 50 g v\u00f4i b\u1ed9t\/c\u00e2y l\u00e0 t\u1ed1t nh\u1ea5t v\u1edbi xu\u1ea5t x\u1ee9 Casino NSW (l\u01b0\u1ee3ng tinh d\u1ea7u\/c\u00e2y b\u1eb1ng 5 l\u1ea7n so v\u1edbi \u0111\u1ed1i ch\u1ee9ng) v\u00e0 c\u00f4ng th\u1ee9c 100g NPK + 50g v\u00f4i b\u1ed9t\/c\u00e2y l\u00e0 t\u1ed1t nh\u1ea5t \u0111\u1ed1i v\u1edbi xu\u1ea5t x\u1ee9 Wasua Road PNG (l\u01b0\u1ee3ng tinh d\u1ea7u\/c\u00e2y b\u1eb1ng 2,3 l\u1ea7n so v\u1edbi \u0111\u1ed1i ch\u1ee9ng).
\nTh\u00ed nghi\u1ec7m \u0111\u1ed9 cao g\u1ed1c ch\u1eb7t sau khi tr\u1ed3ng 4 n\u0103m v\u00e0 thu th\u1eadp s\u1ed1 li\u1ec7u sau \u0111\u00f3 1 n\u0103m cho th\u1ea5y xu\u1ea5t x\u1ee9 West Malam PNG ch\u1eb7t g\u1ed1c \u1edf \u0111\u1ed9 cao 5 cm, xu\u1ea5t x\u1ee9 Wasua Road PNG v\u00e0 Casino NSW ch\u1eb7t g\u1ed1c \u1edf \u0111\u1ed9 cao 15 cm c\u00f3 nhi\u1ec1u ch\u1ed3i v\u00e0 c\u00f3 kh\u1ed1i l\u01b0\u1ee3ng l\u00e1\/c\u00e2y nhi\u1ec1u nh\u1ea5t.
\nT\u1ea1i Ph\u00fa L\u1ed9c (Th\u1eeba Thi\u00ean Hu\u1ebf) h\u00e0m l\u01b0\u1ee3ng tinh d\u1ea7u v\u00e0 t\u1ef7 l\u1ec7 1,8-cineole trong tinh d\u1ea7u Tr\u00e0m n\u0103m g\u00e2n cao nh\u1ea5t v\u00e0 t\u1ef7 l\u1ec7 limonene th\u1ea5p nh\u1ea5t trong m\u00f9a kh\u00f4 (l\u01b0\u1ee3ng m\u01b0a th\u1ea5p).
\nH\u00e0 N\u1ed9i, ng\u00e0y \u2026\u00a0 th\u00e1ng \u2026. n\u0103m 2016<\/p>\n
DISSERTATION INFORMATION ON WEBSITE<\/p>\n
Name of thesis: \u201cResearch on selection and propagation of Melaleuca for high oil content and high 1,8-cineole\u201d.
\nMajor: Genetics and Forest-tree breeding\u00a0\u00a0 Code No: 62 62 02 07
\nPh.D candidate\u2019s name: Khuat Thi Hai Ninh
\nSupervisor:\u00a0 1. Prof. Dr. Le Dinh Kha
\n2. Dr. Phi Hong Hai
\nInstitution: Vietnamese Academy of Forest Sciences<\/p>\n
SUMMARY OF NEW CONTRIBUTIONS OF THE DISSERTATION \n","protected":false},"excerpt":{"rendered":" TH\u00d4NG TIN LU\u1eacN \u00c1N TI\u1ebeN S\u0128 T\u00ean \u0111\u1ec1 t\u00e0i lu\u1eadn \u00e1n: \u201cNghi\u00ean c\u1ee9u ch\u1ecdn gi\u1ed1ng v\u00e0 nh\u00e2n gi\u1ed1ng tr\u00e0m c\u00f3 h\u00e0m l\u01b0\u1ee3ng tinh d\u1ea7u v\u00e0 t\u1ef7 l\u1ec7 1,8-cineole cao\u201d. Chuy\u00ean ng\u00e0nh: Di truy\u1ec1n v\u00e0 Ch\u1ecdn gi\u1ed1ng c\u00e2y l\u00e2m nghi\u1ec7p\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 M\u00e3 s\u1ed1: 62 62 0207 H\u1ecd v\u00e0 t\u00ean NCS: Khu\u1ea5t Th\u1ecb H\u1ea3i Ninh H\u1ecd v\u00e0 t\u00ean […]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[42,66,1],"tags":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/16224"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=16224"}],"version-history":[{"count":4,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/16224\/revisions"}],"predecessor-version":[{"id":17189,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/16224\/revisions\/17189"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=16224"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/categories?post=16224"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/tags?post=16224"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\n1. Selection
\nComplete evaluation of growth variation, oil content and quality of some Melaleuca quinquenervia provenances and clones were shown\u00a0 that the variations of growth,\u00a0 oil content and quality were obvious. The provenances of West Malam PNG (Q15), Wasua Road PNG (Q16), Casino NSW (Q23) and Gympie Qld (Q4) and the clones such as Q4.40 Q4.45, Q15.013, Q15.32 and Q15.38 were well selected. They had the higher oil content than 1.4% and their 1.8-cineole oil was\u00a0 higher than 65%.
\n2. Propagation by tissue culture
\nThe best result of tissue-culture propagation for Melaleuca quinquenervia was sterilizing half-woody shoots by 0.1% HgCl2 twice, the first for four minutes and the second for two minutes. Next,\u00a0 the first regeneration shoot in the MS* medium in which had nitrogen concentration was half of MS, and was supplemented with 0.5mg\/l BAP + 0.2 mg\/l kinetin + 30 g\/l sucrose; the second shoot regeneration in MS* medium was supplemented\u00a0 with 0.5 mg\u00ac\/l BAP + 0.2\u00a0 mg\u00ac\/l kinetin + 30 g\/l glucose (or 20g\/l\u00a0 glucose + 10 g\/l sucrose);\u00a0 the multiplication shoot in MS* medium was supplemented with 0.2 \u2013 0.5 mg\/l BAP + mg\/l K + 0.1 mg\/lNAA; the rooting shoot in MS* medium was supplemented with 0.3 – 0.5 mg\/l NAA + 30 g\/l sucrose (or 20 g\/l\u00a0 glucose + 10 g\/l sucrose). The plantlet was successfully acclimatized in scatterring light for two weeks and planted in soil containers at the nursery. As the results, the contaminated portion of sterilized explants was only 3% and that of clean sprouted explants was from 70 to 83.3%; the rate of shoot regeneration was from 93.3 to 100% and each explants had 3.5 to 4\u00a0 shoots in average; the rate of rooting was higher than 90% and the roots were looked fine. Nursery plantlets have survvied from 86.7 to 91.1%.
\n3. Impact of fertilizer, stump-cutting height and seasonality
\nThe experiment formula of 100g NPK + 200g microbiological organic fertilizer + 50 gram powdered lime per tree was the best for West Malam PNG provenance (oil content per tree was 2.7 times higher than that of control formula); the on supplemental fertilizing Melaleuca quinquenervia for a year has shown that the formula of 200g NPK+ 200g microbiological organic fertilizer + 50g powdered lime per tree was the best for Casino NSW provenance ( oil content per tree was five times higher than control formula) and the formula of 100g NPK + 50g powdered lime per tree\u00a0 was the best for Wasua Road PNG provenance (oil content per tree was 2.7 times higher than that of control formula).
\nSecondly, the 4-year trees were cut in other stump – height and then the shoots were emerged from the stump for one year.\u00a0 The shoot numbers and leaf mass per stump (leaf fresh weight) were counted and measured. The result has showed that stump cutting height was 5cm for West Malam PNG, 1.5cm for Casino NSW and Wasua Road PNG. In our experiment, the number of shoot and leaf mass per tree were the highest among the other experimental formulas.
\nLast, the oil content and 1.8-cineole in Melaleuca quinquenervia is highest and limonene is lowest in the dry season (low rainfall) at Phu Loc district, Thua Thien Hue province.
\nHa Noi, May\u2026\u2026.., 2016
\nChi ti\u1ebft lu\u1eadn \u00e1n v\u00e0 t\u00f3m t\u1eaft lu\u1eadn \u00e1n xem t\u1ea1i \u0111\u00e2y:\u00a0LUAN AN<\/a>\u00a0v\u00e0\u00a0TOM TAT LUAN AN<\/a><\/p>\n