{"id":14440,"date":"2015-03-31T15:25:07","date_gmt":"2015-03-31T08:25:07","guid":{"rendered":"http:\/\/vafs.gov.vn\/vn\/?p=14440"},"modified":"2019-09-24T15:44:19","modified_gmt":"2019-09-24T08:44:19","slug":"bao-cao-ket-qua-nghien-cuu-nghien-cuu-xay-dung-quy-trinh-nuoi-trong-nam-dong-trung-ha-thao-cordyceps-militaris-l-fr-link-co-gia-tri-duoc-lieu-va-thuong-mai-cao","status":"publish","type":"post","link":"https:\/\/vafs.gov.vn\/vn\/bao-cao-ket-qua-nghien-cuu-nghien-cuu-xay-dung-quy-trinh-nuoi-trong-nam-dong-trung-ha-thao-cordyceps-militaris-l-fr-link-co-gia-tri-duoc-lieu-va-thuong-mai-cao\/","title":{"rendered":"B\u00e1o c\u00e1o k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u: Nghi\u00ean c\u1ee9u x\u00e2y d\u1ef1ng quy tr\u00ecnh nu\u00f4i tr\u1ed3ng n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o Cordyceps militaris (L. : Fr.) Link c\u00f3 gi\u00e1 tr\u1ecb d\u01b0\u1ee3c li\u1ec7u v\u00e0 th\u01b0\u01a1ng m\u1ea1i cao"},"content":{"rendered":"
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PGS. TS. Ph\u1ea1m Quang Thu<\/em><\/p>\n

Vi\u1ec7n Khoa h\u1ecdc L\u00e2m nghi\u1ec7p Vi\u1ec7t Nam<\/em><\/p>\n

1. \u0110\u1eb6T V\u1ea4N \u0110\u1ec0<\/p>\n

N\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o \u0111\u01b0\u1ee3c coi l\u00e0 m\u1ed9t d\u01b0\u1ee3c li\u1ec7u truy\u1ec1n th\u1ed1ng c\u1ee7a Trung Qu\u1ed1c, d\u00f9ng ch\u1eefa tr\u1ecb \u0111\u01b0\u1ee3c nhi\u1ec1u b\u1ec7nh nan y. Theo y h\u1ecdc c\u1ed5 truy\u1ec1n c\u1ee7a Trung Qu\u1ed1c, n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o \u0111\u01b0\u1ee3c d\u00f9ng \u0111\u1ec3 \u0111i\u1ec1u tr\u1ecb th\u00e0nh c\u00f4ng c\u00e1c ch\u1ee9ng r\u1ed1i lo\u1ea1n lipit m\u00e1u, vi\u00eam ph\u1ebf qu\u1ea3n m\u1ea1n, hen ph\u1ebf qu\u1ea3n, vi\u00eam th\u1eadn m\u1ea1n t\u00ednh, suy th\u1eadn, r\u1ed1i lo\u1ea1n nh\u1ecbp tim, cao huy\u1ebft \u00e1p, vi\u00eam m\u0169i d\u1ecb \u1ee9ng, vi\u00eam gan B m\u1ea1n t\u00ednh, ung th\u01b0 ph\u1ed5i v\u00e0 thi\u1ec3u n\u0103ng sinh d\u1ee5c. T\u1ea1i vi\u1ec7n Nghi\u00ean c\u1ee9u n\u1ed9i ti\u1ebft Th\u01b0\u1ee3ng H\u1ea3i (Trung Qu\u1ed1c), n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o \u0111\u00e3 \u0111\u01b0\u1ee3c d\u00f9ng \u0111\u1ec3 ch\u1eefa li\u1ec7t d\u01b0\u01a1ng c\u00f3 hi\u1ec7u qu\u1ea3 t\u1ed1t. Nan J.X. v\u00e0 \u0111\u1ed3ng t\u00e1c gi\u1ea3 (2001) ch\u1ee9ng minh n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o ch\u1eefa tr\u1ecb b\u1ec7nh r\u1ed1i lo\u1ea1n ch\u1ee9c n\u0103ng gan r\u1ea5t c\u00f3 hi\u1ec7u qu\u1ea3. T\u00e1c d\u1ee5ng ch\u1ed1ng ung th\u01b0 \u0111\u01b0\u1ee3c \u0111\u1ec1 c\u1eadp \u0111\u1ebfn trong nhi\u1ec1u c\u00f4ng tr\u00ecnh nghi\u00ean c\u1ee9u c\u1ee7a c\u00e1c t\u00e1c gi\u1ea3 \u1edf nhi\u1ec1u qu\u1ed1c gia tr\u00ean th\u1ebf gi\u1edbi. D\u1ecbch chi\u1ebft t\u1eeb th\u1ec3 qu\u1ea3 c\u00f3 t\u00e1c d\u1ee5ng ch\u1ed1ng ung th\u01b0, hi\u1ec7u qu\u1ea3 \u0111\u1ed1i v\u1edbi hai lo\u1ea1i t\u1ebf b\u00e0o m\u00e0ng trong t\u0129nh m\u1ea1ch r\u1ed1n l\u00e0 HT1080 v\u00e0 B16-F10 do c\u00f3 kh\u1ea3 n\u0103ng ch\u1ed1ng l\u1ea1i s\u1ef1 t\u1ea1o th\u00e0nh c\u00e1c m\u1ea1ch m\u00e1u m\u1edbi b\u1eb1ng c\u00e1ch gi\u1ea3m s\u1ef1 bi\u1ec3u hi\u1ec7n c\u1ee7a bFGF, m\u1ed9t trong nh\u1eefng nh\u00e2n t\u1ed1 k\u00edch th\u00edch qu\u00e1 tr\u00ecnh n\u00e0y. Do c\u00f3 vai tr\u00f2 k\u00ecm h\u00e3m qu\u00e1 tr\u00ecnh t\u1ea1o th\u00e0nh c\u00e1c m\u1ea1ch m\u00e1u m\u00e0 c\u00f3 th\u1ec3 ng\u0103n ch\u1eb7n \u0111\u01b0\u1ee3c qu\u00e1 tr\u00ecnh di c\u0103n v\u00e0 s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a t\u1ebf b\u00e0o ung th\u01b0 (Yoo H.S et al<\/i>., 2004). D\u1ecbch chi\u1ebft n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o c\u00f3 t\u00e1c d\u1ee5ng k\u00ecm h\u00e3m s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a t\u1ebf b\u00e0o ung th\u01b0 v\u00fa, ung th\u01b0 ph\u1ed5i (Ahn Y.J. et al<\/i>., 2001). D\u1ecbch chi\u1ebft b\u1eb1ng n\u01b0\u1edbc \u1ea5m c\u00f3 t\u00e1c d\u1ee5ng k\u00ecm h\u00e3m s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a d\u00f2ng t\u1ebf b\u00e0o ung th\u01b0 m\u00e1u \u1edf ng\u01b0\u1eddi b\u1eb1ng c\u00e1ch g\u00e2y ra hi\u1ec7n t\u01b0\u1ee3ng t\u1ef1 ch\u1ebft c\u1ee7a c\u00e1c t\u1ebf b\u00e0o th\u00f4ng qua s\u1ef1 ho\u1ea1t ho\u00e1 enzym caspase-3 (Lee H. et al<\/i>., 2006). Th\u00ed nghi\u1ec7m c\u1ee7a Kim G.Y. v\u00e0 \u0111\u1ed3ng t\u00e1c gi\u1ea3 (2006) c\u0169ng cho k\u1ebft qu\u1ea3 t\u01b0\u01a1ng t\u1ef1 v\u1edbi kh\u1ea3 n\u0103ng \u0111i\u1ec1u tr\u1ecb ung th\u01b0 m\u00e1u c\u1ee7a d\u1ecbch chi\u1ebft t\u1eeb n\u1ea5m. C\u00e1c nh\u00e0 khoa h\u1ecdc c\u0169ng \u0111\u00e3 s\u1eed d\u1ee5ng d\u1ecbch chi\u1ebft t\u1eeb lo\u00e0i n\u1ea5m n\u00e0y \u0111\u1ec3 th\u1eed nghi\u1ec7m tr\u00ean c\u00e1c d\u00f2ng t\u1ebf b\u00e0o b\u00ecnh th\u01b0\u1eddng v\u00e0 c\u00e1c d\u00f2ng t\u1ebf b\u00e0o ung th\u01b0. K\u1ebft qu\u1ea3 cho th\u1ea5y hai d\u00f2ng t\u1ebf b\u00e0o K562 (t\u1ebf b\u00e0o ung th\u01b0 m\u00e1u-leukemia) v\u00e0 Du145 (t\u1ebf b\u00e0o ung th\u01b0 tuy\u1ebfn ti\u1ec1n li\u1ec7t-prostate carcinoma) b\u1ecb \u1ee9c ch\u1ebf m\u1ea1nh b\u1edfi d\u1ecbch chi\u1ebft b\u1eb1ng dung m\u00f4i butanol. Ch\u1ed1ng l\u00e3o ho\u00e1, ch\u1ed1ng c\u00e1c ch\u1ee9ng vi\u00eam t\u1ea5y \u0111\u01b0\u1ee3c th\u1ec3 hi\u1ec7n trong c\u00f4ng tr\u00ecnh nghi\u00ean c\u1ee9u c\u1ee7a Won S.Y v\u00e0 Park E.H. (2005). Ahn Y.J. v\u00e0 \u0111\u1ed3ng t\u00e1c gi\u1ea3 (2000) cho r\u1eb1ng n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o c\u00f3 t\u00e1c d\u1ee5ng ch\u1ed1ng vi\u00eam nhi\u1ec5m k\u00ecm h\u00e3m s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a m\u1ed9t s\u1ed1 vi r\u00fat, vi khu\u1ea9n v\u00e0 n\u1ea5m.<\/p>\n

\u0110\u01b0\u1ee3c s\u1ef1 quan t\u00e2m c\u1ee7a B\u1ed9 N\u00f4ng nghi\u1ec7p v\u00e0 ph\u00e1t tri\u1ec3n n\u00f4ng th\u00f4n v\u00e0 Vi\u1ec7n Khoa h\u1ecdc L\u00e2m nghi\u1ec7p Vi\u1ec7t Nam \u0111\u00e3 c\u1ea5p kinh ph\u00ed v\u00e0 t\u1ea1o \u0111i\u1ec1u ki\u1ec7n cho ch\u00fang t\u00f4i th\u1ef1c hi\u1ec7n \u0111\u1ec1 t\u00e0i v\u1ec1 lo\u00e0i n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o Cordyceps militaris<\/i> v\u1edbi m\u1ee5c ti\u00eau ch\u00ednh l\u00e0 \u0111i\u1ec1u tra th\u00e0nh ph\u1ea7n c\u00e1c lo\u00e0i n\u1ea5m \u0111\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o c\u00f3 \u1edf Vi\u1ec7t Nam, ph\u00e2n l\u1eadp, s\u01b0u t\u1eadp ngu\u1ed3n gen v\u00e0 x\u00e2y d\u1ef1ng \u0111\u01b0\u1ee3c quy tr\u00ecnh nu\u00f4i tr\u1ed3ng lo\u00e0i n\u1ea5m n\u00e0y tr\u00ean gi\u00e1 th\u1ec3. B\u00e1o c\u00e1o n\u00e0y tr\u00ecnh b\u00e0y t\u00f3m t\u1eaft k\u1ebft \u0111\u00e3 \u0111\u1ea1t \u0111\u01b0\u1ee3c trong 2 n\u0103m t\u1eeb n\u0103m 2009 \u0111\u1ebfn n\u0103m 2010.<\/p>\n

2. N\u1ed8I DUNG V\u00c0 PH\u01af\u01a0NG PH\u00c1P NGHI\u00caN C\u1ee8U<\/p>\n

2.1. N\u00f4\u0323i dung nghi\u00ean c\u01b0\u0301u<\/i><\/i><\/h1>\n

– Kh\u1ea3o s\u00e1t thu m\u1eabu n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o \u1edf Vi\u1ec7t Nam v\u00e0 gi\u00e1m \u0111\u1ecbnh m\u1eabu thu \u0111\u01b0\u1ee3c<\/p>\n

– X\u00e2y d\u1ef1ng danh m\u1ee5c ngu\u1ed3n gen c\u00e1c lo\u00e0i n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o<\/p>\n

– Tuy\u1ec3n ch\u1ecdn c\u00e1c ch\u1ee7ng n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o ph\u00e2n l\u1eadp \u0111\u01b0\u1ee3c t\u1eeb Vi\u1ec7t Nam v\u00e0 s\u01b0u t\u1eadp t\u1eeb Trung Qu\u1ed1c v\u00e0 Nh\u1eadt B\u1ea3n c\u00f3 ti\u1ec1m n\u0103ng d\u01b0\u1ee3c li\u1ec7u<\/p>\n

– Nghi\u00ean c\u1ee9u nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3 tr\u00ean gi\u00e1 th\u1ec3 nh\u00e2n t\u1ea1o<\/p>\n

– Nghi\u00ean c\u1ee9u s\u1ea5y kh\u00f4 th\u1ec3 qu\u1ea3<\/p>\n

– Nghi\u00ean c\u1ee9u nu\u00f4i c\u1ea5y sinh kh\u1ed1i h\u1ec7 s\u1ee3i tr\u00ean m\u00f4i tr\u01b0\u1eddng d\u1ecbch th\u1ec3<\/p>\n

– X\u00e2y d\u1ef1ng quy tr\u00ecnh k\u1ef9 thu\u1eadt s\u1ea3n xu\u1ea5t gi\u1ed1ng, c\u00f4ng ngh\u1ec7 nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3 n\u1ea5m Cordyceps militaris<\/i> v\u00e0 x\u00e2y d\u1ef1ng m\u00f4 h\u00ecnh nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3<\/p>\n

2.2. Ph\u01b0\u01a1ng pha\u0301p nghi\u00ean c\u01b0\u0301u<\/i><\/i><\/h1>\n

– Kh\u1ea3o s\u00e1t thu m\u1eabu n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o \u1edf Vi\u1ec7t Nam v\u00e0 gi\u00e1m \u0111\u1ecbnh m\u1eabu thu \u0111\u01b0\u1ee3c<\/p>\n

Kh\u1ea3o s\u00e1t thu m\u1eabu \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n tr\u00ean tuy\u1ebfn \u0111i\u1ec1u tra \u1edf c\u00e1c v\u00f9ng sinh th\u00e1i c\u00f3 \u0111\u1ed9 cao tr\u00ean 1000 m so v\u1edbi m\u1ef1c n\u01b0\u1edbc bi\u1ec3n. C\u00e1c tuy\u1ebfn \u0111i\u1ec1u tra \u0111\u01b0\u1ee3c x\u00e1c l\u1eadp qua c\u00e1c d\u1ea1ng \u0111\u1ecba h\u00ecnh t\u1ea1i c\u00e1c khu v\u1ef1c sau: (i) V\u01b0\u1eddn Qu\u1ed1c gia Ba V\u00ec, Ba V\u00ec, H\u00e0 N\u1ed9i; (ii) V\u01b0\u1eddn Qu\u1ed1c gia Tam \u0110\u1ea3o, Tam \u0110\u1ea3o, V\u0129nh Ph\u00fac; (iii) V\u01b0\u1eddn Qu\u1ed1c gia Ho\u00e0ng Li\u00ean, Sapa v\u00e0 Than Uy\u00ean, L\u00e0o Cai; (iv) Khu b\u1ea3o t\u1ed3n thi\u00ean nhi\u00ean Copia, Thu\u1eadn Ch\u00e2u, S\u01a1n La; (v) V\u01b0\u1eddn Qu\u1ed1c gia B\u1ea1ch M\u00e3, Nam \u0110\u00f4ng v\u00e0 Ph\u00fa L\u1ed9c, Th\u1eeba Thi\u00ean Hu\u1ebf; (vi) V\u01b0\u1eddn Qu\u1ed1c gia Bi Doup-N\u00fai B\u00e0, L\u1ea1c D\u01b0\u01a1ng, L\u00e2m \u0110\u1ed3ng.<\/p>\n

Gi\u00e1m \u0111\u1ecbnh m\u1eabu d\u1ef1a tr\u00ean \u0111\u1eb7c \u0111i\u1ec3m h\u00ecnh th\u00e1i, gi\u1ea3i ph\u1eabu, \u0111\u1ed1i chi\u1ebfu, so s\u00e1nh v\u1edbi chuy\u00ean kh\u1ea3o v\u1ec1 Cordyceps<\/i> c\u1ee7a Gi-Ho Sung et al<\/i>. (2007), Sung Jae Mo (2000), Mao X.L.(2000) v\u00e0 Tsuguo Hongo v\u00e0 Masana Izawa (1994). Vi\u1ec7c ph\u00e2n lo\u1ea1i n\u1ea5m c\u0169ng \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p sinh h\u1ecdc ph\u00e2n t\u1eed, d\u1ef1a tr\u00ean ph\u00e2n \u0111o\u1ea1n 18S ARN ribosom \u0111\u1ed1i v\u1edbi c\u00e1c m\u1eabu n\u1ea5m ph\u00e2n l\u1eadp c\u00e1c n\u1ea5m thu \u0111\u01b0\u1ee3c \u1edf Vi\u1ec7t Nam v\u00e0 s\u01b0u t\u1eadp \u0111\u01b0\u1ee3c t\u1eeb Trung Qu\u1ed1c v\u00e0 Nh\u1eadt B\u1ea3n.<\/p>\n

– X\u00e2y d\u1ef1ng danh m\u1ee5c ngu\u1ed3n gen c\u00e1c lo\u00e0i n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o<\/p>\n

X\u00e2y d\u1ef1ng danh m\u1ee5c tr\u00ean c\u01a1 s\u1edf ph\u00e2n l\u1eadp thu\u1ea7n khi\u1ebft c\u00e1c ch\u1ee7ng n\u1ea5m thu \u0111\u01b0\u1ee3c v\u00e0 nghi\u00ean c\u1ee9u \u0111\u1eb7c \u0111i\u1ec3m sinh h\u1ecdc c\u1ee7a h\u1ec7 s\u1ee3i trong nu\u00f4i c\u1ea5y thu\u1ea7n khi\u1ebft c\u1ee5 th\u1ec3 l\u00e0:<\/p>\n

Th\u00ed nghi\u1ec7m v\u1ec1 m\u00f4i tr\u01b0\u1eddng dinh d\u01b0\u1ee1ng: th\u1ef1c hi\u1ec7n v\u1edbi 4 lo\u1ea1i m\u00f4i tr\u01b0\u1eddng dinh d\u01b0\u1ee1ng kh\u00e1c nhau nh\u01b0: M\u00f4i tr\u01b0\u1eddng PDA (Potato Dextrose Agar), m\u00f4i tr\u01b0\u1eddng PYEG (Peptone Yeast Extract Glucose), m\u00f4i tr\u01b0\u1eddng CSA (Carrot extract Sucrose Agar) v\u00e0 m\u00f4i tr\u01b0\u1eddng MEA (Malt Extract Agar). Ch\u1ec9 ti\u00eau \u0111\u00e1nh gi\u00e1 l\u00e0 sinh tr\u01b0\u1edfng c\u1ee7a h\u1ec7 s\u1ee3i, v\u00e0 s\u1ef1 h\u00ecnh th\u00e0nh b\u00e0o t\u1eed v\u00f4 t\u00ednh (b\u00e0o t\u1eed ch\u1ed3i).<\/p>\n

Th\u00ed nghi\u1ec7m v\u1ec1 \u1ea3nh h\u01b0\u1edfng c\u1ee7a nhi\u1ec7t \u0111\u1ed9 kh\u00f4ng kh\u00ed \u0111\u1ebfn sinh tr\u01b0\u1edfng v\u00e0 ph\u00e1t tri\u1ec3n c\u1ee7a h\u1ec7 s\u1ee3i: th\u00ed nghi\u1ec7m nu\u00f4i c\u1ea5y n\u1ea5m trong c\u00e1c thang nhi\u1ec7t \u0111\u1ed9 kh\u00f4ng kh\u00ed kh\u00e1c nhau: 100<\/sup>C, 150<\/sup>C, 200<\/sup>C, 250<\/sup>C, 300<\/sup>C v\u00e0 350<\/sup>C. Ch\u1ec9 ti\u00eau \u0111\u00e1nh gi\u00e1 l\u00e0 sinh tr\u01b0\u1edfng c\u1ee7a h\u1ec7 s\u1ee3i, v\u00e0 s\u1ef1 h\u00ecnh th\u00e0nh b\u00e0o t\u1eed v\u00f4 t\u00ednh (b\u00e0o t\u1eed ch\u1ed3i).<\/p>\n

Th\u00ed nghi\u1ec7m v\u1ec1 \u1ea3nh h\u01b0\u1edfng c\u1ee7a \u0111\u1ed9 \u1ea9m kh\u00f4ng kh\u00ed \u0111\u1ebfn sinh tr\u01b0\u1edfng v\u00e0 ph\u00e1t tri\u1ec3n c\u1ee7a h\u1ec7 s\u1ee3i: th\u00ed nghi\u1ec7m nu\u00f4i c\u1ea5y n\u1ea5m trong c\u00e1c thang \u1ea9m \u0111\u1ed9 kh\u00f4ng kh\u00ed kh\u00e1c nhau: 60%, 70%, 80%, 90% v\u00e0 100%. Ch\u1ec9 ti\u00eau \u0111\u00e1nh gi\u00e1 l\u00e0 sinh tr\u01b0\u1edfng c\u1ee7a h\u1ec7 s\u1ee3i, v\u00e0 s\u1ef1 h\u00ecnh th\u00e0nh b\u00e0o t\u1eed v\u00f4 t\u00ednh (b\u00e0o t\u1eed ch\u1ed3i).<\/p>\n

Th\u00ed nghi\u1ec7m v\u1ec1 \u1ea3nh h\u01b0\u1edfng c\u1ee7a \u0111\u1ed9 pH m\u00f4i tr\u01b0\u1eddng \u0111\u1ebfn sinh tr\u01b0\u1edfng v\u00e0 ph\u00e1t tri\u1ec3n c\u1ee7a h\u1ec7 s\u1ee3i: th\u00ed nghi\u1ec7m nu\u00f4i c\u1ea5y n\u1ea5m trong c\u00e1c thang pH kh\u00e1c nhau: 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0 v\u00e0 8.5. Ch\u1ec9 ti\u00eau \u0111\u00e1nh gi\u00e1 l\u00e0 sinh tr\u01b0\u1edfng c\u1ee7a h\u1ec7 s\u1ee3i, v\u00e0 s\u1ef1 h\u00ecnh th\u00e0nh b\u00e0o t\u1eed v\u00f4 t\u00ednh (b\u00e0o t\u1eed ch\u1ed3i).<\/p>\n

– Tuy\u1ec3n ch\u1ecdn c\u00e1c ch\u1ee7ng n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o ph\u00e2n l\u1eadp \u0111\u01b0\u1ee3c t\u1eeb Vi\u1ec7t Nam v\u00e0 c\u00e1c ch\u1ee7ng s\u01b0u t\u1eadp \u0111\u01b0\u1ee3c t\u1eeb Trung Qu\u1ed1c v\u00e0 Nh\u1eadt B\u1ea3n c\u00f3 ti\u1ec1m n\u0103ng d\u01b0\u1ee3c li\u1ec7u<\/p>\n

Tuy\u1ec3n ch\u1ecdn c\u00e1c ch\u1ee7ng n\u1ea5m d\u1ef1a v\u00e0o h\u00e0m l\u01b0\u1ee3ng cordycepin v\u00e0 kh\u1ea3 n\u0103ng kh\u00e1ng khu\u1ea9n c\u1ee7a c\u00e1c ch\u1ee7ng n\u1ea5m. X\u00e1c \u0111\u1ecbnh h\u00e0m l\u01b0\u1ee3ng cordycepin b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p LC\/MS v\u1edbi ch\u1ea5t chu\u1ea9n l\u00e0 authentic cordycepin ho\u1eb7c x\u00e1c \u0111\u1ecbnh b\u1eb1ng k\u00fd l\u1ecfng HPLC v\u1edbi \u0111\u1ea7u d\u00f2 UV c\u00f3 b\u01b0\u1edbc s\u00f3ng 260nm. <\/i>S\u1eed d\u1ee5ng c\u00e1c lo\u00e0i n\u1ea5m Candida albicans <\/i>ho\u1eb7c Pythium ultimum <\/i>l\u00e0m \u0111\u1ed1i t\u01b0\u1ee3ng x\u00e1c \u0111\u1ecbnh hi\u1ec7u l\u1ef1c kh\u00e1ng khu\u1ea9n. C\u1ea5y lo\u00e0i n\u1ea5m th\u1eed tr\u00ean c\u00e1c h\u1ed9p l\u1ed3ng \u0111\u00e3 c\u00f3 d\u1ecbch nu\u00f4i c\u1ea5y n\u1ea5m trong c\u00e1c gi\u1ebfng. Nu\u00f4i c\u1ea5y n\u1ea5m trong \u0111i\u1ec1u ki\u1ec7n 250<\/sup>C sau 12 ng\u00e0y x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1eddng k\u00ednh v\u00f2ng \u1ee9c ch\u1ebf. M\u1ed7i lo\u00e0i n\u1ea5m th\u00ed nghi\u1ec7m v\u1edbi 10 h\u1ed9p l\u1ed3ng v\u00e0 3 l\u1ea7n nh\u1eafc l\u1ea1i.<\/p>\n

Th\u00ed nghi\u1ec7m nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3 tr\u00ean gi\u00e1 th\u1ec3 nh\u00e2n t\u1ea1o \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh v\u1edbi c\u00e1c ch\u1ee7ng c\u00f3 ho\u1ea1t t\u00ednh sinh h\u1ecdc nh\u01b0 kh\u1ea3 n\u0103ng kh\u00e1ng khu\u1ea9n, cho h\u00e0m l\u01b0\u1ee3ng cordycepin cao. Ch\u1ecdn c\u00e1c ch\u1ee7ng c\u00f3 n\u0103ng su\u1ea5t cao v\u00e0 th\u1eddi gian nu\u00f4i c\u1ea5y ng\u1eafn, th\u1ec3 qu\u1ea3 to m\u1eadp v\u00e0 m\u00e0u v\u00e0ng da cam, l\u00e0 nh\u1eefng ch\u1ee7ng \u0111ang \u0111\u01b0\u1ee3c th\u1ecb tr\u01b0\u1eddng \u01b0a chu\u1ed9ng.<\/p>\n

– Nghi\u00ean c\u1ee9u nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3 tr\u00ean gi\u00e1 th\u1ec3 nh\u00e2n t\u1ea1o<\/p>\n

Th\u00ed nghi\u1ec7m x\u00e1c \u0111\u1ecbnh gi\u00e1 th\u1ec3 nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3 \u0111\u01b0\u1ee3c chu\u1ea9n b\u1ecb v\u1edbi 7 c\u00f4ng th\u1ee9c sau:<\/p>\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
1<\/td>\nC\u00f4ng th\u1ee9c 1<\/td>\nNh\u1ed9ng t\u1eb1m<\/td>\n<\/tr>\n
2<\/td>\nC\u00f4ng th\u1ee9c 2<\/td>\nC\u01a1m + 1% b\u1ed9t nh\u1ed9ng t\u1eb1m<\/td>\n<\/tr>\n
3<\/td>\nC\u00f4ng th\u1ee9c 3<\/td>\nC\u01a1m + 2% b\u1ed9t nh\u1ed9ng t\u1eb1m<\/td>\n<\/tr>\n
4<\/td>\nC\u00f4ng th\u1ee9c 4<\/td>\nC\u01a1m + 3% b\u1ed9t nh\u1ed9ng t\u1eb1m<\/td>\n<\/tr>\n
5<\/td>\nC\u00f4ng th\u1ee9c 5<\/td>\nB\u1ed9t ng\u00f4 + 1% b\u1ed9t nh\u1ed9ng t\u1eb1m<\/td>\n<\/tr>\n
6<\/td>\nC\u00f4ng th\u1ee9c 6<\/td>\nB\u1ed9t ng\u00f4 + 2% b\u1ed9t nh\u1ed9ng t\u1eb1m<\/td>\n<\/tr>\n
7<\/td>\nC\u00f4ng th\u1ee9c 7<\/td>\nB\u1ed9t ng\u00f4 + 3% b\u1ed9t nh\u1ed9ng t\u1eb1m<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Gi\u00e1 th\u1ec3 \u0111\u01b0\u1ee3c \u0111\u1ef1ng trong c\u00e1c b\u00ecnh nu\u00f4i n\u1ea5m, kh\u1eed tr\u00f9ng \u1edf 1210<\/sup>C, th\u1eddi gian 60 ph\u00fat. C\u1ea5y gi\u1ed1ng n\u1ea5m 7 ng\u00e0y tu\u1ed5i, nu\u00f4i c\u1ea5y \u1edf nhi\u1ec7t \u0111\u1ed9 250<\/sup>C trong t\u1ed1i. Khi s\u1ee3i n\u1ea5m \u0103n k\u00edn gi\u00e1 th\u1ec3 chi\u1ebfu s\u00e1ng v\u1edbi c\u01b0\u1eddng \u0111\u1ed9 700 lux v\u1edbi th\u1eddi gian 12 gi\u1edd\/ng\u00e0y, \u0111\u1ed9 \u1ea9m kh\u00f4ng kh\u00ed 90-95%.<\/p>\n

Th\u00ed nghi\u1ec7m \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh m\u1ed7i c\u00f4ng th\u1ee9c 20 b\u00ecnh nu\u00f4i tr\u1ed3ng, 3 l\u1ea7n l\u1eb7p l\u1ea1i. \u0110\u00e1nh gi\u00e1 k\u1ebft qu\u1ea3 d\u1ef1a tr\u00ean th\u1eddi gian thu ho\u1ea1ch v\u00e0 n\u0103ng su\u1ea5t th\u1ec3 qu\u1ea3 n\u1ea5m thu \u0111\u01b0\u1ee3c.<\/p>\n

– Nghi\u00ean c\u1ee9u s\u1ea5y kh\u00f4 th\u1ec3 qu\u1ea3<\/p>\n

S\u1ea5y kh\u00f4 th\u1ec3 qu\u1ea3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n b\u1eb1ng 3 c\u00f4ng th\u1ee9c: C\u00f4ng th\u1ee9c 1: s\u1ea5y kh\u00f4 \u1edf 400<\/sup>C v\u00e0 C\u00f4ng th\u1ee9c 2: s\u1ea5y kh\u00f4 \u1edf nhi\u1ec7t \u0111\u1ed9 th\u1ea5p c\u00f3 qu\u1ea1t gi\u00f3 v\u1edbi c\u00e1c thang nhi\u1ec7t \u0111\u1ed9 kh\u00e1c nhau: 400<\/sup>C, 350<\/sup>C v\u00e0 300<\/sup>C v\u00e0 c\u00f4ng th\u1ee9c 3: s\u1ea5y kh\u00f4 \u1edf nhi\u1ec7t \u0111\u1ed9 th\u1ea5p b\u1eb1ng m\u00e1y \u0111\u00f4ng kh\u00f4. \u0110\u00e1nh gi\u00e1 ch\u1ea5t l\u01b0\u1ee3ng c\u1ee7a th\u1ec3 qu\u1ea3 th\u00f4ng qua m\u00e0u s\u1eafc s\u1ea3n ph\u1ea9m, \u0111\u1ed9 \u1ea9m t\u01b0\u01a1ng \u0111\u1ed1i v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng th\u1ec3 qu\u1ea3 \u1edf t\u1eebng c\u00f4ng th\u1ee9c th\u00ed nghi\u1ec7m. X\u00e1c \u0111\u1ecbnh th\u1eddi gian s\u1ea5y s\u1ea3n ph\u1ea9m \u0111\u1ea1t \u0111\u1ed9 \u1ea9m t\u01b0\u01a1ng \u0111\u1ed1i l\u00e0 10-12%.<\/p>\n

– Nghi\u00ean c\u1ee9u nu\u00f4i c\u1ea5y sinh kh\u1ed1i h\u1ec7 s\u1ee3i tr\u00ean m\u00f4i tr\u01b0\u1eddng d\u1ecbch th\u1ec3<\/p>\n

Nu\u00f4i c\u1ea5y thu sinh kh\u1ed1i h\u1ec7 s\u1ee3i \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh tr\u00ean 5 m\u00f4i tr\u01b0\u1eddng dinh d\u01b0\u1ee1ng l\u1ecfng v\u1edbi th\u00e0nh ph\u1ea7n nh\u01b0 sau:<\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
1<\/td>\nC\u00f4ng th\u1ee9c 1<\/td>\n40 g\/l\u00edt glucose, 10 g\/l\u00edt peotone, 0.5 g\/l\u00edt KH2<\/sub>PO4<\/sub>, 0.5 g\/l\u00edt K2<\/sub>HPO4<\/sub>:3H2<\/sub>O, v\u00e0\u00a0 0.5 g\/l\u00edt MgSO4<\/sub>.7H2<\/sub>O<\/td>\n<\/tr>\n
2<\/td>\nC\u00f4ng th\u1ee9c 2<\/td>\n40 g\/l\u00edt succose, 10 g\/l\u00edt peotone, 0.5 g\/l\u00edt KH2<\/sub>PO4<\/sub>, 0.5 g\/l\u00edt K2<\/sub>HPO4<\/sub>:3H2<\/sub>O, v\u00e0\u00a0 0.5 g\/l\u00edt MgSO4<\/sub>.7H2<\/sub>O<\/td>\n<\/tr>\n
3<\/td>\nC\u00f4ng th\u1ee9c 3<\/td>\n40 g\/l\u00edt maltose, 10 g\/l\u00edt peotone, 0.5 g\/l\u00edt KH2<\/sub>PO4<\/sub>, 0.5 g\/l\u00edt K2<\/sub>HPO4<\/sub>:3H2<\/sub>O, v\u00e0\u00a0 0.5 g\/l\u00edt MgSO4<\/sub>.7H2<\/sub>O<\/td>\n<\/tr>\n
4<\/td>\nC\u00f4ng th\u1ee9c 4<\/td>\n40 g\/l\u00edt fructose, 10 g\/l\u00edt peotone, 0.5 g\/l\u00edt KH2<\/sub>PO4<\/sub>, 0.5 g\/l\u00edt K2<\/sub>HPO4<\/sub>:3H2<\/sub>O, v\u00e0\u00a0 0.5 g\/l\u00edt MgSO4<\/sub>.7H2<\/sub>O<\/td>\n<\/tr>\n
5<\/td>\nC\u00f4ng th\u1ee9c 5<\/td>\n40 g\/l\u00edt galactose, 10 g\/l\u00edt peotone, 0.5 g\/l\u00edt KH2<\/sub>PO4<\/sub>, 0.5 g\/l\u00edt K2<\/sub>HPO4<\/sub>:3H2<\/sub>O, v\u00e0\u00a0 0.5 g\/l\u00edt MgSO4<\/sub>.7H2<\/sub>O<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Th\u00ed nghi\u1ec7m \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh v\u1edbi 10 b\u00ecnh th\u1ec3 t\u00edch 500 ml, cho 200 ml m\u00f4i tr\u01b0\u1eddng, 3 l\u1ea7n l\u1eb7p; l\u1eafc v\u1edbi t\u1ed1c \u0111\u1ed9 110 v\u00f2ng\/ph\u00fat, nu\u00f4i c\u1ea5y trong 10 ng\u00e0y.\u00a0 Thu ho\u1ea1ch h\u1ec7 s\u1ee3i t\u00ednh tr\u1ecdng l\u01b0\u1ee3ng t\u01b0\u01a1i v\u00e0 kh\u00f4 c\u1ee7a c\u00e1c c\u00f4ng th\u1ee9c th\u00ed nghi\u1ec7m.<\/p>\n

– X\u00e2y d\u1ef1ng quy tr\u00ecnh k\u1ef9 thu\u1eadt s\u1ea3n xu\u1ea5t gi\u1ed1ng, c\u00f4ng ngh\u1ec7 nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3 n\u1ea5m Cordyceps militaris<\/i> v\u00e0 x\u00e2y d\u1ef1ng m\u00f4 h\u00ecnh nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3<\/p>\n

\"15-03-31-Dong<\/a><\/p>\n

M\u00f4 h\u00ecnh nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n t\u1eeb kh\u00e2u s\u1ea3n xu\u1ea5t gi\u1ed1ng, chu\u1ea9n b\u1ecb nguy\u00ean v\u1eadt li\u1ec7u l\u00e0m gi\u00e1 th\u1ec3, nu\u00f4i c\u1ea5y n\u1ea5m tr\u00ean gi\u00e1 th\u1ec3 nh\u00e2n tao thu ho\u1ea1ch \u0111\u01b0\u1ee3c th\u1ec3 qu\u1ea3 th\u00e0nh th\u1ee5c, quy m\u00f4 2000 b\u00ecnh nu\u00f4i c\u1ea5y. S\u1ea3n ph\u1ea9m thu \u0111\u01b0\u1ee3c l\u00e0 th\u1ec3 qu\u1ea3 n\u1ea5m C. militaris<\/i>.<\/i><\/p>\n

3. K\u1ebeT QU\u1ea2 NGHI\u00caN C\u1ee8U<\/p>\n

3.1. Kh\u1ea3o s\u00e1t thu m\u1eabu n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o \u1edf Vi\u1ec7t Nam va\u0300 gia\u0301m \u0111i\u0323nh m\u00e2\u0303u thu \u0111\u01b0\u01a1\u0323c<\/p>\n

3.1.1. Kh\u1ea3o s\u00e1t thu m\u1eabu n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o \u1edf Vi\u1ec7t Nam<\/i><\/p>\n

K\u1ebft qu\u1ea3 gi\u00e1m \u0111\u1ecbnh b\u01b0\u1edbc \u0111\u1ea7u cho th\u1ea5y th\u00e0nh ph\u1ea7n c\u00e1c lo\u00e0i n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o \u1edf Vi\u1ec7t Nam kh\u00e1 phong ph\u00fa g\u1ed3m 37 lo\u00e0i n\u1ea5m thu\u1ed9c 9 chi n\u1ea5m: Achersonia, Akanthomyces, Beauveria, Cordyceps, Gibellula, <\/i>Hymenostilbe, <\/i>Isaria, Metarrhizium, Paecilomyces v\u00e0 Torrubiella.<\/i><\/p>\n

\u0110\u1ed1i v\u1edbi n\u1ea5m \u0111\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o Cordyceps militaris<\/i> thu \u0111\u01b0\u1ee3c 8 m\u1eabu n\u1ea5m \u0111\u00f3 l\u00e0 c\u00e1c c\u00e1c m\u1eabu: HL1, HL2, HL22, HL31, HL34, HL35, BV9 v\u00e0 SL14. C\u00e1c m\u1eabu n\u1ea5m n\u00e0y ch\u1ee7 y\u1ebfu ph\u00e2n b\u1ed1 \u1edf V\u01b0\u1eddn Qu\u1ed1c gia Ho\u00e0ng Li\u00ean, L\u00e0o Cai, 6 m\u1eabu (HL1, HL2, HL22, HL31, HL34, HL35), 1 m\u1eabu v\u01b0\u1eddn qu\u1ed1c gia Ba V\u00ec (BV9) v\u00e0 1 m\u1eabu \u1edf khu b\u1ea3o t\u1ed3n thi\u00ean nhi\u00ean Copia, S\u01a1n La (SL14).<\/p>\n

3.1.2. M\u00f4 t\u1ea3 c\u00e1c m\u1eabu n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o thu \u0111\u01b0\u1ee3c <\/i><\/p>\n

M\u00f4 ta\u0309 va\u0300 x\u00e2y d\u01b0\u0323ng \u0111\u01b0\u01a1\u0323c b\u00f4\u0323 m\u00e2\u0303u g\u00f4\u0300m 243 m\u00e2\u0303u n\u00e2\u0301m \u0110\u00f4ng tru\u0300ng ha\u0323 tha\u0309o thu \u0111\u01b0\u01a1\u0323c \u01a1\u0309 Vi\u00ea\u0323t Nam, trong \u0111\u00f3 c\u00f3 8 m\u1eabu n\u1ea5m \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 Cordyceps militaris<\/i><\/p>\n

3.2. X\u00e2y d\u1ef1ng danh m\u1ee5c ngu\u1ed3n gen c\u00e1c lo\u00e0i n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o<\/p>\n

3.2.1. Ph\u00e2n l\u1eadp thu\u1ea7n khi\u1ebft n\u1ea5m t\u1eeb th\u1ec3 qu\u1ea3 thu \u0111\u01b0\u1ee3c \u1edf Vi\u1ec7t Nam<\/i><\/p>\n

Ph\u00e2n l\u1eadp thu\u1ea7n khi\u1ebft \u0111\u01b0\u1ee3c 108 ch\u1ee7ng n\u1ea5m thu\u1ed9c c\u00e1c lo\u00e0i n\u1ea5m Cordyceps<\/i> spp. v\u00e0 8 ch\u1ee7ng n\u1ea5m thu\u1ed9c Cordyceps militaris<\/i> (HL1, HL2, HL22, HL31, HL34, HL35, SL14, BV9)<\/p>\n

3.2.2. S\u01b0u t\u1eadp c\u00e1c ch\u1ee7ng gi\u1ed1ng \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o t\u1eeb Trung Qu\u1ed1c v\u00e0 Nh\u1eadt B\u1ea3n<\/i><\/p>\n

S\u01b0u t\u00e2\u0323p \u0111\u01b0\u01a1\u0323c 4 chu\u0309ng gi\u00f4\u0301ng \u0110\u00f4ng tru\u0300ng ha\u0323 tha\u0309o: 1 chu\u0309ng t\u01b0\u0300 Trung Qu\u00f4\u0301c (CM); 3 chu\u0309ng t\u01b0\u0300 Nh\u00e2\u0323t Ba\u0309n (F1010, F1012, F1080).<\/p>\n

3.2.3. Nghi\u00ean c\u1ee9u \u0111\u1eb7c \u0111i\u1ec3m sinh h\u1ecdc c\u1ee7a h\u1ec7 s\u1ee3i trong nu\u00f4i c\u1ea5y thu\u1ea7n khi\u1ebft<\/i><\/p>\n

M\u00f4i tr\u01b0\u01a1\u0300ng dinh d\u01b0\u01a1\u0303ng t\u00f4\u0301t nh\u00e2\u0301t cho s\u01b0\u0323 sinh tr\u01b0\u01a1\u0309ng va\u0300 pha\u0301t tri\u00ea\u0309n cu\u0309a h\u00ea\u0323 s\u01a1\u0323i la\u0300: PDA + 10% nh\u00f4\u0323ng t\u0103\u0300m. Nhi\u00ea\u0323t \u0111\u00f4\u0323 kh\u00f4ng khi\u0301 thi\u0301ch h\u01a1\u0323p cho h\u00ea\u0323 s\u01a1\u0323i n\u00e2\u0301m sinh tr\u01b0\u01a1\u0309ng va\u0300 pha\u0301t tri\u00ea\u0309n t\u01b0\u0300 150<\/sup>C-250<\/sup>C. \u0110\u00f4\u0323 \u00e2\u0309m kh\u00f4ng khi\u0301 t\u01b0\u0300 80% – 85% thi\u0301ch h\u01a1\u0323p cho s\u01b0\u0323 sinh tr\u01b0\u01a1\u0309ng va\u0300 pha\u0301t tri\u00ea\u0309n cu\u0309a h\u00ea\u0323 s\u01a1\u0323i. pH m\u00f4i tr\u01b0\u01a1\u0300ng t\u01b0\u0300 4,5 \u2013 6,5 thi\u0301ch h\u01a1\u0323p cho s\u01b0\u0323 sinh tr\u01b0\u01a1\u0309ng va\u0300 pha\u0301t tri\u00ea\u0309n cu\u0309a h\u00ea\u0323 s\u01a1\u0323i.<\/p>\n

3.2.4. Nghi\u00ean c\u1ee9u b\u1ea3o qu\u1ea3n gi\u1ed1ng g\u1ed1c<\/i><\/p>\n

Ph\u01b0\u01a1ng ph\u00e1p l\u01b0u tr\u1eef v\u00e0 b\u1ea3o qu\u1ea3n gi\u1ed1ng n\u1ea5m Cordyceps militaris<\/i> trong n\u01b0\u1edbc c\u1ea5t v\u00f4 tr\u00f9ng b\u0103ng k\u00edn \u01a1\u0309 5o<\/sup>C b\u1ea3o \u0111\u1ea3m an to\u00e0n ch\u1ea5t l\u01b0\u1ee3ng sinh tr\u01b0\u1edfng c\u1ee7a n\u1ea5m \u00edt nh\u1ea5t trong th\u1eddi gian 17 th\u00e1ng.<\/p>\n

3.3. Nghi\u00ean c\u1ee9u tuy\u1ec3n ch\u1ecdn c\u00e1c ch\u1ee7ng n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o ph\u00e2n l\u1eadp \u0111\u01b0\u1ee3c t\u1eeb Vi\u1ec7t Nam v\u00e0 s\u01b0u t\u1eadp t\u1eeb Trung Qu\u1ed1c v\u00e0 Nh\u1eadt B\u1ea3n c\u00f3 ti\u1ec1m n\u0103ng d\u01b0\u1ee3c li\u1ec7u<\/p>\n

Ca\u0301c chu\u0309ng CM, HL2, HL22, HL34, HL35, F1080 co\u0301 kha\u0309 n\u0103ng kha\u0301ng khu\u00e2\u0309n Bacillus subtilis <\/i>va\u0300 kha\u0301ng n\u00e2\u0301m Fusarium oxysporum.<\/i> 3 ch\u1ee7ng kh\u00e1ng t\u1ed1t nh\u1ea5t l\u00e0 CM, HL2 v\u00e0 F1080.<\/p>\n

3.4. Nghi\u00ean c\u1ee9u nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3 n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o tr\u00ean gi\u00e1 th\u1ec3 nh\u00e2n t\u1ea1o<\/p>\n

3.4.1. Nghi\u00ean c\u1ee9u t\u1ea1o gi\u1ed1ng s\u1ea3n xu\u1ea5t t\u1eeb gi\u1ed1ng g\u1ed1c<\/i><\/p>\n

M\u00f4i tr\u01b0\u01a1\u0300ng nh\u00e2n gi\u00f4\u0301ng sa\u0309n xu\u00e2\u0301t t\u01b0\u0300 gi\u00f4\u0301ng g\u00f4\u0301c t\u00f4\u0301t nh\u00e2\u0301t la\u0300: PDA + 10% nh\u00f4\u0323ng t\u0103\u0300m<\/p>\n

3.4.2. Nghi\u00ean c\u1ee9u x\u00e1c \u0111\u1ecbnh th\u00e0nh ph\u1ea7n gi\u00e1 th\u1ec3 nh\u00e2n t\u1ea1o nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3 <\/i><\/p>\n

Tha\u0300nh ph\u00e2\u0300n gia\u0301 th\u00ea\u0309 nh\u00e2n ta\u0323o nu\u00f4i tr\u00f4\u0300ng th\u00ea\u0309 qua\u0309 cho n\u0103ng su\u00e2\u0301t cao nh\u00e2\u0301t la\u0300: C\u00f4ng th\u01b0\u0301c, c\u01a1m + 2% nh\u00f4\u0323ng t\u0103\u0300m t\u01b0\u01a1i.<\/p>\n

3.4.3. Nghi\u00ean c\u1ee9u \u1ea3nh h\u01b0\u1edfng c\u1ee7a pH gia\u0301 th\u00ea\u0309 \u0111\u1ebfn sinh tr\u01b0\u1edfng c\u1ee7a h\u1ec7 s\u1ee3i v\u00e0 s\u1ef1 h\u00ecnh th\u00e0nh th\u1ec3 qu\u1ea3<\/i><\/p>\n

pH gia\u0301 th\u00ea\u0309 t\u01b0\u0300 6,5-8 thi\u0301ch h\u01a1\u0323p cho s\u01b0\u0323 sinh tr\u01b0\u01a1\u0309ng cu\u0309a m\u00e2\u0300m th\u00ea\u0309 qua\u0309.<\/p>\n

3.4.4. Nghi\u00ean c\u1ee9u x\u00e1c \u0111\u1ecbnh c\u00e1c nh\u00e2n t\u1ed1 sinh th\u00e1i ch\u00ednh cho qu\u00e1 tr\u00ecnh nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3<\/i><\/p>\n

K\u00ea\u0301t qua\u0309 nghi\u00ean c\u01b0\u0301u v\u00ea\u0300 nhi\u00ea\u0323t \u0111\u00f4\u0323 kh\u00f4ng khi\u0301 a\u0309nh h\u01b0\u01a1\u0309ng \u0111\u00ea\u0301n sinh tr\u01b0\u01a1\u0309ng cu\u0309a m\u00e2\u0300m th\u00ea\u0309 qua\u0309 cho th\u00e2\u0301y v\u01a1\u0301i nhi\u00ea\u0323t \u0111\u00f4\u0323 200<\/sup>C – 250<\/sup>C sinh tr\u01b0\u01a1\u0309ng cu\u0309a m\u00e2\u0300m th\u00ea\u0309 qua\u0309 \u0111a\u0323t cao nh\u00e2\u0301t<\/p>\n

\u01a0\u0309 \u0111\u00f4\u0323 \u00e2\u0309m kh\u00f4ng khi\u0301 t\u01b0\u0300 85%-90% la\u0300 k\u00ea\u0301t qua\u0309 nghi\u00ea\u0323n c\u01b0\u0301u v\u00ea\u0300 \u0111\u00f4\u0323 \u00e2\u0309m cu\u0309a gia\u0301 th\u00ea\u0309 cho sinh tr\u01b0\u01a1\u0309ng t\u00f4\u0301t nh\u00e2\u0301t<\/p>\n

V\u01a1\u0301i c\u01b0\u01a1\u0300ng \u0111\u00f4\u0323 chi\u00ea\u0301u sa\u0301ng 700 lux sinh tr\u01b0\u01a1\u0309ng cu\u0309a m\u00e2\u0300m th\u00ea\u0309 qua\u0309 la\u0300 t\u00f4\u0301t nh\u00e2\u0301t.<\/p>\n

3.5. Tham quan h\u1ecdc t\u1eadp t\u1ea1i Nh\u1eadt B\u1ea3n<\/p>\n

\u0110a\u0303 tham quan, ho\u0323c t\u00e2\u0323p ta\u0323i Nh\u00e2\u0323t Ba\u0309n v\u00e0 ho\u0323c \u0111\u01b0\u01a1\u0323c ph\u01b0\u01a1ng pha\u0301p gi\u00e1m \u0111\u1ecbnh c\u00e1c ch\u1ee7ng n\u1ea5m b\u1eb1ng k\u1ef9 thu\u1eadt ADN.<\/p>\n

4. K\u1ebeT LU\u1eacN<\/p>\n

4.1. K\u1ebft lu\u1eadn<\/p>\n

Thu th\u1eadp \u0111\u01b0\u1ee3c 243 m\u1eabu n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o thu\u1ed9c chi Cordyceps v\u00e0 m\u1ed9t s\u1ed1 lo\u00e0i kh\u00e1c \u1edf giai \u0111o\u1ea1n v\u00f4 t\u00ednh, trong \u0111\u00f3 c\u00f3 8 m\u1eabu n\u1ea5m \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 Cordyceps militaris<\/i><\/p>\n

Ph\u00e2n l\u1eadp \u0111\u01b0\u1ee3c 8 ch\u1ee7ng n\u1ea5m Cordyceps militaris<\/i> v\u00e0 s\u01b0u t\u1eadp \u0111\u01b0\u1ee3c 4 ch\u1ee7ng n\u1ea5m t\u1eeb n\u01b0\u1edbc ngo\u00e0i: Cordyceps militaris<\/i> CM t\u1eeb Trung Qu\u1ed1c,\u00a0 Cordyceps militaris<\/i> F1080, Cordyceps militaris<\/i> F1010 v\u00e0 Cordyceps militaris<\/i> F1012 t\u1eeb Nh\u1eadt B\u1ea3n<\/p>\n

Ch\u1ee7ng Cordyceps militaris<\/i> CM, HL2 v\u00e0 ch\u1ee7ng F1080 c\u00f3 kh\u1ea3 n\u0103ng kh\u00e1ng khu\u1ea9n v\u00e0 kh\u00e1ng n\u1ea5m s\u1ee3i t\u1ed1t nh\u1ea5t.<\/p>\n

M\u00f4i tr\u01b0\u01a1\u0300ng sa\u0309n xu\u00e2\u0301t gi\u00f4\u0301ng la\u0300 m\u00f4i tr\u01b0\u01a1\u0300ng Nh\u1ed9ng t\u1eb1m, c\u01a1m + 2% nh\u00f4\u0323ng t\u0103\u0300m t\u01b0\u01a1i, pH gia\u0301 th\u00ea\u0309 t\u01b0\u0300 6,5-8, nhi\u1ec7t \u0111\u1ed9 nu\u00f4i c\u1ea5y 20-25 \u0111\u1ed9 C, \u1ea9m \u0111\u1ed9 kh\u00f4ng kh\u00ed 85-90%, c\u01b0\u1eddng \u0111\u1ed9 \u00e1nh s\u00e1ng l\u00e0 700Lux thi\u0301ch h\u01a1\u0323p cho s\u01b0\u0323 sinh tr\u01b0\u01a1\u0309ng cu\u0309a m\u00e2\u0300m th\u00ea\u0309 qua\u0309 v\u00e0 th\u1ec3 qu\u1ea3 ph\u00e1t tri\u1ec3n.<\/p>\n

4.2. Ki\u1ebfn ngh\u1ecb<\/p>\n

C\u0103n c\u1ee9 Th\u00f4ng t\u01b0 s\u1ed1 67\/2010\/TT-BNNPTNT ng\u00e0y 17\/11\/2010 c\u1ee7a B\u1ed9 N\u00f4ng nghi\u1ec7p v\u00e0 Ph\u00e1t tri\u1ec3n n\u00f4ng th\u00f4n v\u1ec1 vi\u1ec7c thay \u0111\u1ed5i \u0111\u01a1n gi\u00e1 c\u00f4ng lao \u0111\u1ed9ng k\u1ef9 thu\u1eadt. N\u0103m 2011, \u0111\u1ec1 t\u00e0i ki\u1ebfn ngh\u1ecb \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh v\u00e0 c\u00e2n \u0111\u1ed1i kinh ph\u00ed gi\u1eefa c\u00e1c kho\u1ea3n m\u1ee5c cho h\u1ee3p l\u00fd \u0111\u1ec3 ti\u1ebfp t\u1ee5c nghi\u00ean c\u1ee9u, ho\u00e0n thi\u1ec7n quy tr\u00ecnh v\u00e0 x\u00e2y d\u1ef1ng m\u00f4 h\u00ecnh nu\u00f4i tr\u1ed3ng th\u1ec3 qu\u1ea3.<\/p>\n

5. T\u00c0I LI\u1ec6U THAM KH\u1ea2O<\/p>\n

Ti\u1ebfng vi\u1ec7t<\/p>\n

Pham Quang Thu, 2009. \u0110i\u1ec1u tra ph\u00e1t hi\u1ec7n n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o Cordyceps nutans<\/i> Pat. Ph\u00e2n b\u1ed1 \u1edf khu b\u1ea3o t\u1ed3n thi\u00ean nhi\u00ean T\u00e2y Y\u00ean T\u1eed, S\u01a1n \u0110\u1ed9ng, B\u1eafc Giang. T\u1ea1p ch\u00ed N\u00f4ng nghi\u1ec7p & Ph\u00e1t tri\u1ec3n n\u00f4ng th\u00f4n, B\u1ed9 N\u00f4ng nghi\u1ec7p & Ph\u00e1t tri\u1ec3n n\u00f4ng th\u00f4n, s\u1ed1 4, trang 91-94.<\/p>\n

Ti\u1ebfng anh<\/p>\n

Bao, Z.D., Wu, Z.G., and Zheng, F. (1994). [Amelioration of aminoglycoside nephrotoxicity by Cordyceps Sinensis <\/i>in old patients]. Chinese Journal of Integrated Medicine <\/i>14: 259,271-273.<\/p>\n

Berne, R.M., 1980: The role of Adenosine in the regulation of coronary blood flow. Circ. Res. 47: 807-813, 1980.<\/p>\n

Bok JW, Lermer L, Chilton J, Klingeman HG, Towers GH., (1999) Antitumor sterols from the mycelia of Cordyceps sinensis. <\/i>Phytochemistry 1999 Aug;51(7):891-8.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

PGS. TS. Ph\u1ea1m Quang Thu Vi\u1ec7n Khoa h\u1ecdc L\u00e2m nghi\u1ec7p Vi\u1ec7t Nam 1. \u0110\u1eb6T V\u1ea4N \u0110\u1ec0 N\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o \u0111\u01b0\u1ee3c coi l\u00e0 m\u1ed9t d\u01b0\u1ee3c li\u1ec7u truy\u1ec1n th\u1ed1ng c\u1ee7a Trung Qu\u1ed1c, d\u00f9ng ch\u1eefa tr\u1ecb \u0111\u01b0\u1ee3c nhi\u1ec1u b\u1ec7nh nan y. Theo y h\u1ecdc c\u1ed5 truy\u1ec1n c\u1ee7a Trung Qu\u1ed1c, n\u1ea5m \u0110\u00f4ng tr\u00f9ng h\u1ea1 th\u1ea3o \u0111\u01b0\u1ee3c d\u00f9ng […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[21,72],"tags":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/14440"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=14440"}],"version-history":[{"count":4,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/14440\/revisions"}],"predecessor-version":[{"id":19183,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/14440\/revisions\/19183"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=14440"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/categories?post=14440"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/tags?post=14440"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}