{"id":14435,"date":"2015-03-26T14:41:23","date_gmt":"2015-03-26T07:41:23","guid":{"rendered":"http:\/\/vafs.gov.vn\/vn\/?p=14435"},"modified":"2019-09-24T15:44:45","modified_gmt":"2019-09-24T08:44:45","slug":"bao-cao-ket-qua-de-tai-nghien-cuu-cong-nghe-san-xuat-che-pham-vi-sinh-vat-hon-hop-dang-vien-nen-cho-bach-dan-va-thong-tren-cac-lap-dia-thoai-hoa-ngheo-chat-dinh-duong","status":"publish","type":"post","link":"https:\/\/vafs.gov.vn\/vn\/bao-cao-ket-qua-de-tai-nghien-cuu-cong-nghe-san-xuat-che-pham-vi-sinh-vat-hon-hop-dang-vien-nen-cho-bach-dan-va-thong-tren-cac-lap-dia-thoai-hoa-ngheo-chat-dinh-duong\/","title":{"rendered":"B\u00e1o c\u00e1o k\u1ebft qu\u1ea3 \u0111\u1ec1 t\u00e0i: Nghi\u00ean c\u1ee9u c\u00f4ng ngh\u1ec7 s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m vi sinh v\u1eadt h\u1ed7n h\u1ee3p d\u1ea1ng vi\u00ean n\u00e9n cho b\u1ea1ch \u0111\u00e0n v\u00e0 th\u00f4ng tr\u00ean c\u00e1c l\u1eadp \u0111\u1ecba tho\u00e1i ho\u00e1, ngh\u00e8o ch\u1ea5t dinh d\u01b0\u1ee1ng"},"content":{"rendered":"

PGS. TS. Ph\u1ea1m Quang Thu<\/em><\/p>\n

Vi\u1ec7n Khoa h\u1ecdc L\u00e2m nghi\u1ec7p Vi\u1ec7t Nam<\/em><\/p>\n

1. \u0110\u1eb6T V\u1ea4N \u0110\u1ec0<\/h3>\n

C\u00e2y th\u00f4ng v\u00e0 c\u00e2y b\u1ea1ch \u0111\u00e0n \u0111ang l\u00e0 nh\u1eefng lo\u00e0i c\u00e2y tr\u1ed3ng r\u1eebng ph\u1ed5 bi\u1ebfn \u1edf n\u01b0\u1edbc ta. C\u1ea3 th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n \u0111\u1ec1u mang l\u1ea1i c\u00e1c gi\u00e1 tr\u1ecb v\u1ec1 kinh t\u1ebf v\u00e0 m\u00f4i tr\u01b0\u1eddng cho ng\u01b0\u1eddi tr\u1ed3ng r\u1eebng. V\u1edbi l\u1ee3i \u00edch v\u1ec1 m\u1eb7t kinh t\u1ebf n\u00ean di\u1ec7n t\u00edch r\u1eebng tr\u1ed3ng c\u1ee7a hai lo\u00e0i n\u00e0y \u1edf Vi\u1ec7t Nam \u0111\u00e3 l\u00ean \u0111\u1ebfn g\u1ea7n 1,5 tri\u1ec7u ha. Tuy nhi\u00ean hi\u1ec7n nay nhi\u1ec1u di\u1ec7n t\u00edch r\u1eebng tr\u1ed3ng c\u00e2y th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n \u0111ang b\u1ecb suy gi\u1ea3m v\u1ec1 sinh tr\u01b0\u1edfng v\u00e0 b\u1ecb b\u1ec7nh h\u1ea1i t\u1ea5n c\u00f4ng. C\u00e2y th\u00f4ng th\u01b0\u1eddng b\u1ecb b\u1ec7nh th\u1ed1i c\u1ed5 r\u1ec5 g\u00e2y h\u1ea1i ngay t\u1eeb giai \u0111o\u1ea1n v\u01b0\u1eddn \u01b0\u01a1m v\u00e0 \u1edf c\u1ea3 r\u1eebng tr\u1ed3ng, c\u00e2y b\u1ea1ch \u0111\u00e0n th\u01b0\u1eddng b\u1ecb c\u00e1c b\u1ec7nh v\u1ec1 l\u00e1 (ch\u00e1y l\u00e1, kh\u00f4 ng\u1ecdn, \u0111\u1ed1m l\u00e1\u2026) \u1edf giai \u0111o\u1ea1n r\u1eebng tr\u1ed3ng. H\u01a1n n\u1eefa, hi\u1ec7n nay nhi\u1ec1u di\u1ec7n t\u00edch tr\u1ed3ng th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n \u0111\u01b0\u1ee3c tr\u1ed3ng tr\u00ean c\u00e1c l\u1eadp \u0111\u1ecba tho\u00e1i h\u00f3a, ngh\u00e8o ch\u1ea5t dinh d\u01b0\u1ee1ng. T\u1ea5t c\u1ea3 c\u00e1c \u1ea3nh h\u01b0\u1edfng tr\u00ean \u0111\u1ec1u d\u1eabn \u0111\u1ebfn s\u1ef1 suy gi\u1ea3m v\u1ec1 sinh tr\u01b0\u1edfng v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng r\u1eebng tr\u1ed3ng. \u0110\u1ec3 kh\u1eafc ph\u1ee5c t\u00ecnh tr\u1ea1ng tr\u00ean, ng\u01b0\u1eddi ta \u0111\u00e3 s\u1eed d\u1ee5ng c\u00e1c ch\u1ebf ph\u1ea9m c\u00f3 ngu\u1ed3n g\u1ed1c VSV, th\u00e2n thi\u1ec7n v\u1edbi m\u00f4i tr\u01b0\u1eddng \u0111\u1ec3 b\u00f3n cho c\u00e2y nh\u1eb1m t\u0103ng n\u0103ng su\u1ea5t v\u00e0 c\u00f3 t\u00e1c d\u1ee5ng c\u1ea3i t\u1ea1o \u0111\u1ea5t, kh\u00f4ng g\u00e2y \u00f4 nhi\u1ec5m ho\u1eb7c l\u00e0m tho\u00e1i h\u00f3a th\u00eam \u0111\u1ea5t.<\/p>\n

Ch\u1ebf ph\u1ea9m VSV d\u1ea1ng vi\u00ean n\u00e9n b\u00f3n cho c\u00e2y r\u1eebng nh\u1eb1m t\u0103ng sinh tr\u01b0\u1edfng c\u1ee7a c\u00e2y, gi\u1ea3m thi\u1ec3u t\u1ef7 l\u1ec7 b\u1ecb b\u1ec7nh c\u1ee7a c\u00e2y ch\u1ee7 \u0111\u00e3 \u0111\u01b0\u1ee3c nhi\u1ec1u n\u01b0\u1edbc tr\u00ean th\u1ebf gi\u1edbi nghi\u00ean c\u1ee9u v\u00e0 s\u1ea3n xu\u1ea5t d\u1ea1ng th\u01b0\u01a1ng m\u1ea1i nh\u01b0 \u1edf M\u1ef9, Canada. \u1ede Vi\u1ec7t Nam c\u0169ng \u0111\u00e3 c\u00f3 c\u00e1c nghi\u00ean c\u00fau v\u1ebf s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m cho c\u00e2y th\u00f4ng, b\u1ea1ch \u0111\u00e0n, ch\u1ee7 y\u1ebfu cho giai \u0111o\u1ea1n c\u00e2y con \u1edf v\u01b0\u1eddn \u01b0\u01a1m.<\/p>\n

\u0110\u1ec3 ph\u00e1t huy t\u1ed1t hi\u1ec7u l\u1ef1c c\u1ee7a ch\u1ebf ph\u1ea9m v\u00e0 \u0111\u01b0a ch\u1ebf ph\u1ea9m \u00e1p d\u1ee5ng v\u00e0o s\u1ea3n xu\u1ea5t c\u1ea7n c\u00f3 b\u01b0\u1edbc c\u1ea3i ti\u1ebfn quy tr\u00ecnh s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m t\u1eeb d\u1ea1ng b\u1ed9t sang d\u1ea1ng vi\u00ean n\u00e9n \u0111\u1ec3 gi\u1eef b\u00e0o t\u1eed t\u1ed3n t\u1ea1i l\u00e2u h\u01a1n trong \u0111\u1ea5t khi ch\u01b0a thi\u1ebft l\u1eadp \u0111\u01b0\u1ee3c m\u1ed1i c\u1ed9ng sinh v\u1edbi c\u00e2y ch\u1ee7 v\u00e0 h\u1ea1 gi\u00e1 th\u00e0nh s\u1ea3n ph\u1ea9m. Ch\u1ebf ph\u1ea9m h\u1ed7n h\u1ee3p c\u00f3 kho\u1ea3ng 30% Potassium polyacrylamide, m\u1ed9t ch\u1ea5t keo gi\u1eef n\u01b0\u1edbc. \u0110\u00e2y l\u00e0 m\u1ed9t s\u1ea3n ph\u1ea9m m\u1edbi l\u1ea7n \u0111\u1ea7u ti\u00ean s\u1ebd \u0111\u01b0\u1ee3c nghi\u00ean c\u1ee9u v\u00e0 \u00e1p d\u1ee5ng cho c\u00e2y l\u00e2m nghi\u1ec7p \u1edf Vi\u1ec7t Nam. Ch\u1ebf ph\u1ea9m d\u1ea1ng vi\u00ean n\u00e9n ngo\u00e0i th\u00e0nh ph\u1ea7n VSV l\u00e0 n\u1ea5m c\u1ed9ng sinh, VSV ph\u00e2n gi\u1ea3i l\u00e2n, VSV n\u1ed9i sinh \u0111\u1ed1i kh\u00e1ng v\u1edbi n\u1ea5m g\u00e2y b\u1ec7nh, keo gi\u1eef n\u01b0\u1edbc c\u00f2n c\u00f3 kho\u1ea3ng 50 – 60% l\u00e0 ch\u1ea5t h\u1eefu c\u01a1 v\u00e0 b\u1ed9t kho\u00e1ng l\u00e2n v\u00f4 c\u01a1 gi\u00fap cho h\u1ec7 VSV \u0111\u1ea5t c\u1ee7a ch\u1ebf ph\u1ea9m t\u1ed3n t\u1ea1i v\u00e0 ph\u00e1t tri\u1ec3n v\u00e0 cung c\u1ea5p dinh d\u01b0\u1ee1ng cho c\u00e2y.<\/p>\n

Hi\u1ec7n nay, theo th\u1ed1ng k\u00ea c\u1ee7a B\u1ed9 N\u00f4ng nghi\u1ec7p v\u00e0 Ph\u00e1t tri\u1ec3n n\u00f4ng th\u00f4n, c\u1ea3 n\u01b0\u1edbc c\u00f3 t\u1edbi 9,34 tri\u1ec7u ha \u0111\u1ea5t hoang ho\u00e1, trong \u0111\u00f3 c\u00f3 kho\u1ea3ng 7,85 tri\u1ec7u ha ch\u1ecbu t\u00e1c \u0111\u1ed9ng m\u1ea1nh b\u1edfi sa m\u1ea1c ho\u00e1, ch\u1ee7 y\u1ebfu l\u00e0 \u0111\u1ea5t tr\u1ed1ng, \u0111\u1ed3i n\u00fai tr\u1ecdc, b\u1ea1c m\u00e0u. V\u00ec v\u1eady, vi\u1ec7c tuy\u1ec3n ch\u1ecdn c\u00e1c ch\u1ee7ng VSV c\u00f3 l\u1ee3i v\u00e0 t\u1ea1o ch\u1ebf ph\u1ea9m d\u1ea1ng h\u1ed7n h\u1ee3p d\u01b0\u1edbi d\u1ea1ng vi\u00ean n\u00e9n bao g\u1ed3m b\u00e0o t\u1eed n\u1ea5m ngo\u1ea1i c\u1ed9ng sinh, VSV ph\u00e2n gi\u1ea3i l\u00e2n s\u1ebd l\u00e0 \u0111i\u1ec3m quan tr\u1ecdng trong vi\u1ec7c b\u1ed5 sung d\u1ea7n d\u1ea7n ngu\u1ed3n VSV c\u00f3 l\u1ee3i cho c\u00e2y tr\u1ed3ng l\u00e0 c\u1ea7n thi\u1ebft \u0111\u1ec3 s\u1eed d\u1ee5ng \u0111\u1ea1t hi\u1ec7u qu\u1ea3 cao nh\u1eefng l\u1eadp \u0111\u1ecba tho\u00e1i ho\u00e1, ngh\u00e8o ch\u1ea5t dinh d\u01b0\u1ee1ng v\u00e0 \u0111ang ch\u1ecbu s\u1ef1 sa m\u1ea1c ho\u00e1.<\/p>\n

\"15-03-26-Viennen\"<\/a>2. \u00a0N\u1ed8I DUNG V\u00c0 PH\u01af\u01a0NG PH\u00c1P NGHI\u00caN C\u1ee8U<\/h3>\n

2.1. N\u1ed9i dung nghi\u00ean c\u1ee9u<\/h3>\n

– Nghi\u00ean c\u1ee9u ph\u00e2n l\u1eadp m\u1edbi, tuy\u1ec3n ch\u1ecdn v\u00e0 \u0111\u1ecbnh danh ngu\u1ed3n VSV \u0111\u1ec3 s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m vi sinh h\u1ed7n h\u1ee3p ph\u1ee5c v\u1ee5 ch\u0103m s\u00f3c c\u00e2y tr\u1ed3ng b\u1ea1ch \u0111\u00e0n v\u00e0 th\u00f4ng.<\/p>\n

– Nghi\u00ean c\u1ee9u kh\u1ea3 n\u0103ng t\u1eadp h\u1ee3p ch\u1ee7ng<\/p>\n

– <\/b>Nghi\u00ean c\u1ee9u nh\u00e2n sinh kh\u1ed1i c\u00e1c ch\u1ee7ng VSV v\u00e0 thu h\u00e1i, s\u01a1 ch\u1ebf v\u00e0 l\u00e0m kh\u00f4 b\u00e0o t\u1eed n\u1ea5m c\u1ed9ng sinh Pisolithus tinctorius<\/i><\/p>\n

– <\/b>X\u00e2y d\u1ef1ng quy tr\u00ecnh c\u00f4ng ngh\u1ec7 v\u00e0 s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m h\u1ed7n h\u1ee3p ph\u1ee5c v\u1ee5 tr\u1ed3ng v\u00e0 ch\u0103m s\u00f3c c\u00e2y th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n<\/p>\n

– <\/b>Nghi\u00ean c\u1ee9u b\u1ea3o qu\u1ea3n ch\u1ebf ph\u1ea9m<\/p>\n

–\u00a0 <\/b>X\u00e2y d\u1ef1ng m\u00f4 h\u00ecnh s\u1eed d\u1ee5ng hi\u1ec7u qu\u1ea3 ch\u1ebf ph\u1ea9m v\u00e0 \u0111\u00e1nh gi\u00e1 hi\u1ec7u l\u1ef1c c\u1ee7a ch\u1ebf ph\u1ea9m \u0111\u1ed1i v\u1edbi b\u1ea1ch \u0111\u00e0n v\u00e0 th\u00f4ng<\/p>\n

– <\/b>X\u00e2y d\u1ef1ng quy tr\u00ecnh s\u1eed d\u1ee5ng ch\u1ebf ph\u1ea9m h\u1ed7n h\u1ee3p ph\u1ee5c v\u1ee5 tr\u1ed3ng v\u00e0 ch\u0103m s\u00f3c c\u00e2y th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n<\/p>\n

2.2. Ph\u01b0\u01a1ng ph\u00e1p nghi\u00ean c\u1ee9u<\/h3>\n

– N\u1ea5m c\u1ed9ng sinh:<\/b><\/p>\n

N\u1ea5m c\u1ed9ng sinh \u0111\u01b0\u1ee3c ph\u00e2n l\u1eadp v\u00e0 nu\u00f4i c\u1ea5y tr\u00ean m\u00f4i tr\u01b0\u1eddng MMN, Marx (1969); PACH (Pachlewski\u00a0 medium, 1974); FDA (Ferry & Das, 1968). Tuy\u1ec3n ch\u1ecdn n\u1ea5m c\u1ed9ng sinh \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n trong nu\u00f4i c\u1ea5y thu\u1ea7n khi\u1ebft in-vitro. \u0110\u1ecbnh danh n\u1ea5m c\u1ed9ng sinh v\u1edbi th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n: d\u1ef1a tr\u00ean m\u00f4 t\u1ea3 c\u1ee7a chuy\u00ean kh\u1ea3o v\u1ec1 c\u00e1c lo\u00e0i n\u1ea5m c\u1ed9ng sinh c\u1ee7a Brundrett v\u00e0 c\u1ed9ng s\u1ef1 (1996).<\/p>\n

– Vi sinh v\u1eadt ph\u00e2n gi\u1ea3i l\u00e2n<\/b><\/p>\n

Ph\u00e2n l\u1eadp VSV ph\u00e2n gi\u1ea3i l\u00e2n theo ph\u01b0\u01a1ng ph\u00e1p pha lo\u00e3ng t\u1edbi h\u1ea1n tr\u00ean m\u00f4i tr\u01b0\u1eddng Pikovskaya c\u00f3 Ca3<\/sub>(PO4<\/sub>)2<\/sub>. Tuy\u1ec3n chon c\u00e1c ch\u1ee7ng c\u00f3 hi\u1ec7u l\u1ef1c d\u1ef1a v\u00e0o \u0111\u01b0\u1eddng k\u00ednh c\u1ee7a v\u00f2ng ph\u00e2n gi\u1ea3i l\u00e2n.<\/p>\n

– \u00a0VSV n\u1ed9i sinh \u0111\u1ed1i kh\u00e1ng v\u1edbi n\u1ea5m g\u00e2y b\u1ec7nh h\u1ea1i th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n<\/b><\/p>\n

Ph\u00e2n l\u1eadp \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n tr\u00ean m\u00f4i tr\u01b0\u1eddng PBS v\u00e0 m\u00f4i tr\u01b0\u1eddng PDA. Tuy\u1ec3n ch\u1ecdn VSV \u0111\u1ed1i kh\u00e1ng v\u1edbi n\u1ea5m g\u00e2y b\u1ec7nh (Fusarium oxysporum<\/i>; Cylindrocladium quinqueseptatum<\/i> v\u00e0 Cryptosporiopsis eucalypti<\/i>) d\u1ef1a v\u00e0o \u0111\u01b0\u1eddng k\u00ednh v\u00f2ng \u1ee9c ch\u1ebf.<\/p>\n

– Nghi\u00ean c\u1ee9u kh\u1ea3 n\u0103ng t\u1eadp h\u1ee3p ch\u1ee7ng<\/b><\/p>\n

\u0110\u00e1nh gi\u00e1 s\u1ef1 thay \u0111\u1ed5i m\u1eadt \u0111\u1ed9 t\u1ebf b\u00e0o c\u1ee7a c\u00e1c ch\u1ee7ng VSV trong c\u00e1c ch\u1ebf ph\u1ea9m h\u1ed7n h\u1ee3p b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p pha lo\u00e3ng t\u1edbi h\u1ea1n v\u00e0 c\u1ea5y trang tr\u00ean m\u00f4i tr\u01b0\u1eddng th\u00edch h\u1ee3p v\u1edbi t\u1eebng lo\u1ea1i VSV.<\/p>\n

– Nghi\u00ean c\u1ee9u nh\u00e2n sinh kh\u1ed1i c\u00e1c ch\u1ee7ng VSV v\u00e0 ph\u01b0\u01a1ng ph\u00e1p l\u00e0m kh\u00f4 b\u00e0o t\u1eed n\u1ea5m c\u1ed9ng sinh Pisolithus tinctorius<\/i><\/b><\/p>\n

Th\u1eed nghi\u1ec7m v\u1edbi c\u00e1c lo\u1ea1i m\u00f4i tr\u01b0\u1eddng dinh d\u01b0\u1ee1ng kh\u00e1c nhau. T\u1ed1c \u0111\u1ed9 l\u1eafc: 0, 100, 150 v\u00e0 200 v\u00f2ng\/ph\u00fat. Th\u1eddi gian l\u1eafc: 24 gi\u1edd, 48 gi\u1edd, 72 gi\u1edd, 96 gi\u1edd v\u00e0 120 gi\u1edd.<\/p>\n

– X\u00e2y d\u1ef1ng quy tr\u00ecnh c\u00f4ng ngh\u1ec7 v\u00e0 s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m h\u1ed7n h\u1ee3p ph\u1ee5c v\u1ee5 tr\u1ed3ng v\u00e0 ch\u0103m s\u00f3c c\u00e2y th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n<\/b><\/p>\n

Quy tr\u00ecnh c\u00f4ng ngh\u1ec7 v\u00e0 s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m VSV h\u1ed7n h\u1ee3p cho c\u00e2y th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n \u0111\u01b0\u1ee3c \u0111\u01b0\u1ee3c x\u00e2y d\u1ef1ng tr\u00ean c\u00f4ng th\u1ee9c c\u1ee7a Mycro Tree Saver.<\/p>\n

– Nghi\u00ean c\u1ee9u b\u1ea3o qu\u1ea3n ch\u1ebf ph\u1ea9m<\/b><\/p>\n

\u0110\u00e1nh gi\u00e1 s\u1ef1 thay \u0111\u1ed5i m\u1eadt \u0111\u1ed9 t\u1ebf b\u00e0o c\u1ee7a c\u00e1c ch\u1ee7ng VSV trong c\u00e1c ch\u1ebf ph\u1ea9m h\u1ed7n h\u1ee3p b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p pha lo\u00e3ng t\u1edbi h\u1ea1n v\u00e0 c\u1ea5y trang tr\u00ean m\u00f4i tr\u01b0\u1eddng th\u00edch h\u1ee3p v\u1edbi t\u1eebng lo\u1ea1i VSV theo ph\u01b0\u01a1ng th\u1ee9c b\u1ea3o qu\u1ea3n v\u00e0 th\u1eddi gian b\u1ea3o qu\u1ea3n 30 ng\u00e0y, 60 ng\u00e0y, 90 ng\u00e0y v\u00e0 120 ng\u00e0y.<\/p>\n

– X\u00e2y d\u1ef1ng m\u00f4 h\u00ecnh s\u1eed d\u1ee5ng c\u00f3 hi\u1ec7u qu\u1ea3 ch\u1ebf ph\u1ea9m v\u00e0 \u0111\u00e1nh gi\u00e1 hi\u1ec7u l\u1ef1c c\u1ee7a ch\u1ebf ph\u1ea9m<\/b><\/p>\n

Th\u00ed nghi\u1ec7m nhi\u1ec5m ch\u1ebf ph\u1ea9m \u0111\u1edbi v\u1edbi s\u1ea3n xu\u1ea5t c\u00e2y con \u1edf v\u01b0\u1eddn \u01b0\u01a1m: Th\u00ed nghi\u1ec7m \u0111\u01b0\u1ee3c th\u1eed nghi\u1ec7m \u0111\u1ed1i v\u1edbi 2 lo\u00e0i th\u00f4ng (Th\u00f4ng m\u00e3 v\u0129 \u2013 Pinus massoniana<\/i> \u00a0v\u00e0 Th\u00f4ng nh\u1ef1a – Pinus merkusii<\/i>) v\u00e0 2 lo\u00e0i b\u1ea1ch \u0111\u00e0n (B\u1ea1ch \u0111\u00e0n tr\u1eafng \u2013 Eucalyptus camaldulensis<\/i> v\u00e0\u00a0 b\u1ea1ch \u0111\u00e0n n\u00e2u –\u00a0 Eucalyptus urophyla<\/i>). \u0110\u1ed1i v\u1edbi m\u1ed7i lo\u00e0i c\u00e2y, th\u00ed nghi\u1ec7m g\u1ed3m 4 c\u00f4ng th\u1ee9c kh\u00e1c nhau v\u1edbi 3 l\u1ea7n l\u1eb7p, b\u1ed1 tr\u00ed theo kh\u1ed1i ng\u1eabu nhi\u00ean \u0111\u1ea7y \u0111\u1ee7. M\u1ed7i c\u00f4ng th\u1ee9c th\u00ed nghi\u1ec7m s\u1eed d\u1ee5ng 40 c\u00e2y con.<\/p>\n

Th\u00ed nghi\u1ec7m nhi\u1ec5m ch\u1ebf ph\u1ea9m \u0111\u1ed1i v\u1edbi r\u1eebng tr\u1ed3ng: X\u00e2y d\u1ef1ng 3 ha r\u1eebng tr\u1ed3ng th\u00ed nghi\u1ec7m b\u1ea1ch \u0111\u00e0n v\u00e0 2 ha r\u1eebng tr\u1ed3ng th\u00ed nghi\u1ec7m th\u00f4ng, trong \u0111\u00f3 2 ha b\u1ea1ch \u0111\u00e0n n\u00e2u d\u00f2ng PN14, 1 ha b\u1ea1ch d\u00f2ng U6 v\u00e0 2 ha th\u00f4ng nh\u1ef1a. M\u00f4 h\u00ecnh r\u1eebng tr\u1ed3ng th\u00ed nghi\u1ec7m b\u1ea1ch \u0111\u00e0n (d\u00f2ng PN14) t\u1ea1i B\u1eafc Giang: M\u1eadt \u0111\u1ed9 tr\u1ed3ng r\u1eebng l\u00e0 1660 c\u00e2y\/ha, c\u00e2y c\u00e1ch c\u00e2y 2 m\u00e9t, h\u00e0ng c\u00e1ch hang 3 m\u00e9t (S\u01a1 \u0111\u1ed3 tr\u1ed3ng r\u1eebng xem Ph\u1ee5 l\u1ee5c 1). Th\u00ed nghi\u1ec7m \u0111\u01b0\u1ee3c chia l\u00e0m 4 c\u00f4ng th\u1ee9c v\u1edbi 4 l\u1ea7n l\u1eb7p l\u1ea1i, b\u1ed1 tr\u00ed theo kh\u1ed1i ng\u1eabu nhi\u00ean \u0111\u1ea7y \u0111\u1ee7.<\/p>\n

– X\u00e2y d\u1ef1ng h\u01b0\u1edbng d\u1eabn s\u1eed d\u1ee5ng ch\u1ebf ph\u1ea9m h\u1ed7n h\u1ee3p ph\u1ee5c v\u1ee5 tr\u1ed3ng v\u00e0 ch\u0103m s\u00f3c c\u00e2y th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n <\/b><\/p>\n

H\u01b0\u1edbng d\u1eabn k\u1ef9 thu\u1eadt s\u1eed d\u1ee5ng ch\u1ebf ph\u1ea9m \u0111\u01b0\u1ee3c x\u00e2y d\u1ef1ng tr\u00ean c\u01a1 s\u1edf th\u00ed nghi\u1ec7m nhi\u1ec5m ch\u1ebf ph\u1ea9m cho c\u00e2y con \u1edf v\u01b0\u1eddn \u01b0\u01a1m v\u00e0 r\u1eebng tr\u1ed3ng.<\/p>\n

3. K\u1ebeT QU\u1ea2 NGHI\u00caN C\u1ee8U<\/b><\/b><\/p>\n

3.1. Nghi\u00ean c\u1ee9u ph\u00e2n l\u1eadp m\u1edbi, tuy\u1ec3n ch\u1ecdn v\u00e0 \u0111\u1ecbnh danh ngu\u1ed3n VSV <\/b><\/b><\/p>\n

\u0110\u00e3 tuy\u1ec3n ch\u1ecdn \u0111\u01b0\u1ee3c 10 ch\u1ee7ng n\u1ea5m c\u1ed9ng sinh v\u1edbi th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n. C\u00e1c lo\u00e0i n\u1ea5m c\u1ed9ng sinh \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0: Pisolithus tinctorius, Scleroderma bovista, Scleroderma <\/i>areolatum, <\/i>Russula emetica, <\/i>Tylopilus chromupes, Lactarius hygrophroides, Lepista sordida, Russula rosea, <\/i>Suillus collinitus. <\/i>Ph\u00e2n l\u1eadp \u0111\u01b0\u1ee3c 15 ch\u1ee7ng VSV ph\u00e2n gi\u1ea3i l\u00e2n, c\u00e1c ch\u1ee7ng \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh thu\u1ed9c lo\u00e0i: Burkholderia cenocepacia, Burkholderia mana, <\/i>\u00a0Burkholderia bryophila, <\/i>Burkholderia tropicalis<\/i>.\u00a0 (c\u00f3 kh\u1ea3 n\u0103ng ph\u00e2n gi\u1ea3i l\u00e2n cao so v\u1edbi \u0111\u1ed1i ch\u1ee9ng, g\u1ea5p t\u1eeb 5,8 \u0111\u1ebfn 11,3 l\u1ea7n), 10 ch\u1ee7ng VSV \u0111\u1ed1i kh\u00e1ng n\u1ea5m g\u00e2y b\u1ec7nh th\u1ed1i c\u1ed5 r\u1ec5 c\u00e2y th\u00f4ng, 5 ch\u1ee7ng VSV \u0111\u1ed1i kh\u00e1ng n\u1ea5m g\u00e2y b\u1ec7nh ch\u00e1y l\u00e1 b\u1ea1ch \u0111\u00e0n v\u00e0 5 ch\u1ee7ng VSV \u0111\u1ed1i kh\u00e1ng n\u1ea5m g\u00e2y b\u1ec7nh \u0111\u1ed1m l\u00e1 b\u1ea1ch \u0111\u00e0n (c\u00f3 \u0111\u01b0\u1eddng k\u00ednh \u1ee9c ch\u1ebf \u0111\u1ed1i v\u1edbi c\u00e1c lo\u00e0i n\u1ea5m g\u00e2y b\u1ec7nh t\u1eeb 21 \u0111\u1ebfn 29 mm). C\u00e1c ch\u1ee7ng vi sinh v\u1eadt \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0: Bacillus subtilis, <\/i>Bacillus amyloliquefaciens<\/i>.<\/p>\n

3.2. Nghi\u00ean c\u1ee9u kh\u1ea3 n\u0103ng t\u1eadp h\u1ee3p ch\u1ee7ng<\/h3>\n

Trong ch\u1ebf ph\u1ea9m vi\u00ean n\u00e9n, c\u00e1c ch\u1ee7ng c\u00f3 th\u1ec3 t\u1ed3n t\u1ea1i c\u00f9ng v\u1edbi nhau v\u00e0 kh\u00f4ng c\u00f3 hi\u1ec7n t\u01b0\u1ee3ng th\u1ef1c khu\u1ea9n. M\u1eadt \u0111\u1ed9 t\u1ebf b\u00e0o c\u1ee7a c\u00e1c ch\u1ee7ng VSV h\u1eefu hi\u1ec7u kh\u00f4ng thay \u0111\u1ed5i sau 4 tu\u1ea7n v\u00e0 gi\u1ea3m nh\u1eb9 sau 8 tu\u1ea7n. Ho\u1ea1t t\u00ednh sinh h\u1ecdc c\u1ee7a c\u00e1c ch\u1ee7ng VSV sau khi t\u1eadp h\u1ee3p ch\u1ee7ng v\u1eabn b\u1ea3o t\u1ed3n \u0111\u01b0\u1ee3c kh\u1ea3 n\u0103ng ph\u00e2n gi\u1ea3i l\u00e2n kh\u00f3 tan v\u00e0 \u0111\u1ed1i kh\u00e1ng v\u1edbi c\u00e1c lo\u1ea1i n\u1ea5m b\u1ec7nh.<\/p>\n

3.3. Nghi\u00ean c\u1ee9u nh\u00e2n sinh kh\u1ed1i c\u00e1c ch\u1ee7ng VSV v\u00e0 thu h\u00e1i, s\u01a1 ch\u1ebf v\u00e0 l\u00e0m kh\u00f4 b\u00e0o t\u1eed n\u1ea5m c\u1ed9ng sinh Pisolithus tinctorius<\/i><\/h3>\n

M\u00f4i tr\u01b0\u1eddng dinh d\u01b0\u1ee1ng cho nh\u00e2n sinh kh\u1ed1i c\u00e1c ch\u1ee7ng VSV ph\u00e2n gi\u1ea3i l\u00e2n l\u00e0 Pikovskaya, VSV \u0111\u1ed1i kh\u00e1ng n\u1ea5m g\u00e2y b\u1ec7nh l\u00e0 PD broth, t\u1ed1c \u0111\u1ed9 l\u1eafc t\u1ed1i \u01b0u l\u00e0 200 v\u00f2ng\/ph\u00fat trong 72 gi\u1edd \u1edf nhi\u1ec7t \u0111\u1ed9 25o<\/sup>C.<\/p>\n

3.4. X\u00e2y d\u1ef1ng quy tr\u00ecnh c\u00f4ng ngh\u1ec7 v\u00e0 s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m h\u1ed7n h\u1ee3p ph\u1ee5c v\u1ee5 tr\u1ed3ng v\u00e0 ch\u0103m s\u00f3c c\u00e2y th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n<\/h3>\n

C\u00e1c thi\u1ebft b\u1ecb ph\u1ee5c v\u1ee5 cho s\u1ea3n xu\u1ea5t vi\u00ean n\u00e9n: M\u00e1y nghi\u1ec1n GF300, m\u00e1y tr\u1ed9n CH150, m\u00e1y d\u1eadp vi\u00ean THP. Th\u00e0nh ph\u1ea7n ch\u1ebf ph\u1ea9m vi\u00ean n\u00e9n: b\u1ed9t apatit: 20 %, m\u00f9n: 30%, \u0111\u1ea5t s\u00e9t: 5%, potassium polyacrylamide: 35 %, keo t\u1ea1o h\u1ea1t: 7% v\u00e0 VSV.<\/p>\n

3.5. B\u1ea3o qu\u1ea3n ch\u1ebf ph\u1ea9m<\/h3>\n

Ch\u1ebf ph\u1ea9m b\u1ea3o qu\u1ea3n b\u1eb1ng t\u00fai ni l\u00f4ng \u0111\u1ec3 \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng, kh\u00f4ng thay \u0111\u1ed5i sau th\u1eddi gian b\u1ea3o qu\u1ea3n 30 ng\u00e0y, m\u1eadt \u0111\u1ed9 t\u1ebf b\u00e0o h\u1eefu hi\u1ec7u v\u1eabn \u0111\u1ea3m b\u1ea3o tr\u00ean 109<\/sup> CFU\/g ch\u1ebf ph\u1ea9m v\u00e0 gi\u1ea3m nh\u1eb9 sau 60 – 90 ng\u00e0y, sau th\u1eddi gian 120 ng\u00e0y m\u1eadt \u0111\u1ed9 t\u1ebf b\u00e0o gi\u1ea3m m\u1ea1nh h\u01a1n, t\u1eeb 109<\/sup> xu\u1ed1ng 107<\/sup> cfu\/g.<\/p>\n

5.6.\u00a0 X\u00e2y d\u1ef1ng m\u00f4 h\u00ecnh s\u1eed d\u1ee5ng ch\u1ebf ph\u1ea9m v\u00e0 \u0111\u00e1nh gi\u00e1 hi\u1ec7u l\u1ef1c c\u1ee7a ch\u1ebf ph\u1ea9m<\/h3>\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a ch\u1ebf ph\u1ea9m \u0111\u1ed1i v\u1edbi Th\u00f4ng m\u00e3 v\u0129 v\u00e0 Th\u00f4ng nh\u1ef1a \u1edf v\u01b0\u1eddn \u01b0\u01a1m sau 4 th\u00e1ng cho th\u1ea5y c\u00f4ng th\u1ee9c 1 c\u00f3 hi\u1ec7u l\u1ef1c cao nh\u1ea5t. So v\u1edbi \u0111\u1ed1i ch\u1ee9ng, chi\u1ec1u cao trung b\u00ecnh cao h\u01a1n g\u1ea5p 1,45 l\u1ea7n, t\u1ef7 l\u1ec7 b\u1ecb b\u1ec7nh c\u1ee7a c\u00e1c c\u00f4ng th\u1ee9c b\u00f3n ch\u1ebf ph\u1ea9m gi\u1ea3m t\u1eeb 90 \u0111\u1ebfn 93% v\u00e0 t\u1ef7 l\u1ec7 c\u1ed9ng sinh \u0111\u1ea1t t\u1eeb 70,83 \u0111\u1ebfn 98,3%.<\/p>\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a ch\u1ebf ph\u1ea9m \u0111\u1ed1i v\u1edbi b\u1ea1ch \u0111\u00e0n camal v\u00e0 b\u1ea1ch \u0111\u00e0n n\u00e2u \u1edf v\u01b0\u1eddn \u01b0\u01a1m sau 4 th\u00e1ng c\u0169ng cho th\u1ea5y c\u00f4ng th\u1ee9c 1 c\u00f3 hi\u1ec7u l\u1ef1c cao nh\u1ea5t. So v\u1edbi \u0111\u1ed1i ch\u1ee9ng, chi\u1ec1u cao trung b\u00ecnh cao h\u01a1n g\u1ea5p 1,4 l\u1ea7n, t\u1ef7 l\u1ec7 b\u1ecb b\u1ec7nh c\u1ee7a c\u00e1c c\u00f4ng th\u1ee9c b\u00f3n ch\u1ebf ph\u1ea9m gi\u1ea3m t\u1eeb 88 \u0111\u1ebfn 93% v\u00e0 t\u1ef7 l\u1ec7 c\u1ed9ng sinh \u0111\u1ea1t t\u1eeb 80,83% \u0111\u1ebfn 93,3%.<\/p>\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a ch\u1ebf ph\u1ea9m \u0111\u1ed1i v\u1edbi d\u00f2ng b\u1ea1ch \u0111\u00e0n PN14 t\u1ea1i r\u1eebng tr\u1ed3ng th\u00ed nghi\u1ec7m sau 24 th\u00e1ng tu\u1ed5i: C\u00f4ng th\u1ee9c 1 (b\u00f3n 7g ch\u1ebf ph\u1ea9m\/c\u00e2y) c\u00f3 \u1ea3nh h\u01b0\u1edfng t\u1ed1t nh\u1ea5t, so v\u1edbi c\u00f4ng th\u1ee9c \u0111\u1ed1i ch\u1ee9ng, l\u00e0m t\u0103ng sinh tr\u01b0\u1edfng v\u1ec1 chi\u1ec1u cao 28,7%, \u0111\u01b0\u1eddng k\u00ednh l\u00e0 38,2% v\u00e0 gi\u1ea3m t\u1ef7 l\u1ec7 c\u00e2y b\u1ecb b\u1ec7nh l\u00e0 83,06% %.<\/p>\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a ch\u1ebf ph\u1ea9m \u0111\u1ed1i v\u1edbi d\u00f2ng b\u1ea1ch \u0111\u00e0n U6 t\u1ea1i r\u1eebng tr\u1ed3ng th\u00ed nghi\u1ec7m sau 20 th\u00e1ng tu\u1ed5i: C\u00f4ng th\u1ee9c 1 (b\u00f3n 7g ch\u1ebf ph\u1ea9m\/c\u00e2y) c\u00f3 \u1ea3nh h\u01b0\u1edfng t\u1ed1t nh\u1ea5t, so v\u1edbi c\u00f4ng th\u1ee9c \u0111\u1ed1i ch\u1ee9ng, l\u00e0m t\u0103ng sinh tr\u01b0\u1edfng v\u1ec1 chi\u1ec1u cao 55,2%, \u0111\u01b0\u1eddng k\u00ednh l\u00e0 38,9%, v\u00e0 gi\u1ea3m t\u1ef7 l\u1ec7 c\u00e2y b\u1ecb b\u1ec7nh l\u00e0 85,09 %.<\/p>\n

\u1ea2nh h\u01b0\u1edfng c\u1ee7a ch\u1ebf ph\u1ea9m \u0111\u1ed1i v\u1edbi Th\u00f4ng nh\u1ef1a t\u1ea1i r\u1eebng tr\u1ed3ng th\u00ed nghi\u1ec7m sau 19 th\u00e1ng tu\u1ed5i: C\u00f4ng th\u1ee9c 1 (b\u00f3n 7g ch\u1ebf ph\u1ea9m\/c\u00e2y) c\u00f3 \u1ea3nh h\u01b0\u1edfng t\u1ed1t nh\u1ea5t, so v\u1edbi c\u00f4ng th\u1ee9c \u0111\u1ed1i ch\u1ee9ng, l\u00e0m t\u0103ng sinh tr\u01b0\u1edfng v\u1ec1 chi\u1ec1u cao 16,1%, \u0111\u01b0\u1eddng k\u00ednh l\u00e0 40,1%, v\u00e0 gi\u1ea3m t\u1ef7 l\u1ec7 c\u00e2y b\u1ecb b\u1ec7nh l\u00e0 88,81 %.<\/p>\n

5.7.<\/b> X\u00e2y d\u1ef1ng quy tr\u00ecnh s\u1eed d\u1ee5ng ch\u1ebf ph\u1ea9m h\u1ed7n h\u1ee3p ph\u1ee5c v\u1ee5 tr\u1ed3ng v\u00e0 ch\u0103m s\u00f3c c\u00e2y th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n<\/b><\/p>\n

Quy tr\u00ecnh \u0111\u1ec1 c\u1eadp d\u1ebfn c\u00e1c v\u1ea5n \u0111\u1ec1 sau: \u0111\u1ed1i t\u01b0\u1ee3ng \u0111\u1ec3 b\u00f3n ch\u1ebf ph\u1ea9m, x\u00e1c \u0111\u1ecbnh th\u1eddi \u0111i\u1ec3m b\u00f3n ch\u1ebf ph\u1ea9m, x\u00e1c \u0111\u1ecbnh li\u1ec1u l\u01b0\u1ee3ng ch\u1ebf ph\u1ea9m c\u1ea7n b\u00f3n v\u00e0 c\u00e1ch b\u00f3n ch\u1ebf ph\u1ea9m<\/p>\n

4. K\u1ebeT LU\u1eacN<\/h3>\n

– \u0110\u1ec1 t\u00e0i \u0111\u00e3 tuy\u1ec3n ch\u1ecdn v\u00e0 l\u01b0u gi\u1eef \u0111\u01b0\u1ee3c c\u00e1c ch\u1ee7ng vi sinh v\u1eadt n\u1ea5m c\u1ed9ng sinh, vi sinh v\u1eadt ph\u00e2n gi\u1ea3i l\u00e2n v\u00e0 vi sinh v\u1eadt \u0111\u1ed1i kh\u00e1ng v\u1edbi n\u1ea5m g\u00e2y b\u1ec7nh. \u0110\u00e2y l\u00e0 nh\u1eefng ch\u1ee7ng c\u00f3 hi\u1ec7u l\u1ef1c m\u1ea1nh th\u00edch h\u1ee3p v\u1edbi \u0111\u1ea5t c\u00f3 \u0111\u1ed9 pH th\u1ea5p.<\/p>\n

– C\u00e1c ch\u1ee7ng vi khu\u1ea9n c\u00f3 kh\u1ea3 n\u0103ng t\u1eadp h\u1ee3p v\u00e0 kh\u00f4ng co s\u1ef1 c\u1ea1nh tranh, ho\u1ea1t l\u1ef1c kh\u00f4ng b\u1ecb \u1ea3nh h\u01b0\u1edfng.<\/p>\n

– M\u00f4i tr\u01b0\u1eddng dinh d\u01b0\u1ee1ng cho nh\u00e2n sinh kh\u1ed1i c\u00e1c ch\u1ee7ng VSV ph\u00e2n gi\u1ea3i l\u00e2n l\u00e0 Pikovskaya, VSV \u0111\u1ed1i kh\u00e1ng n\u1ea5m g\u00e2y b\u1ec7nh l\u00e0 PD broth, t\u1ed1c \u0111\u1ed9 l\u1eafc t\u1ed1i \u01b0u l\u00e0 200 v\u00f2ng\/ph\u00fat trong 72 gi\u1edd \u1edf nhi\u1ec7t \u0111\u1ed9 25o<\/sup>C.<\/p>\n

– Ch\u1ebf ph\u1ea9m c\u00f3 t\u00e1c d\u1ee5ng t\u1ed1t \u0111\u1ebfn t\u0103ng sinh tr\u01b0\u1edfng, ch\u1ed1ng ch\u1ecbu b\u1ec7nh cho c\u00e2y con th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n \u1edf v\u01b0\u1eddn \u01b0\u01a1m v\u00e0 r\u1eebng tr\u1ed3ng.<\/p>\n

5. T\u00c0I LI\u1ec6U THAM KH\u1ea2O<\/h3>\n

Ti\u1ebfng Anh<\/b><\/p>\n

Brundrett M., Bougher N., Dell B., Grove T. and Malajczuk N. (1996) Working with Mycorrhizas in Forestry and Agriculture. ACIAR Monograph 32. 374p.<\/p>\n

Nautiyal C.S. (1999) An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol. Lett. 170: 65-270.<\/p>\n

Pajand Nejad and Paul A. Johson (2000) Endophytic Bacteria Induce Growth Promotion and Wilt Disease Suppression in Oilseed Rape and Tomato. Biological Control 18, 208-215.<\/p>\n

Pikovskaya RI (1948) Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiologiya 17 :362\u2013370.<\/p>\n

Rai M. K. (Editor) (2006) Handbook of Microbiol Biofertilizers.\u00a0 Food Product Press. An Imprint of The Haworth Press, Inc.<\/p>\n

Ti\u1ebfng Vi\u1ec7t<\/b><\/p>\n

Nguy\u1ec5n Th\u1ecb Th\u00fay Nga v\u00e0 Ph\u1ea1m Quang Thu (2009) Ph\u00e2n l\u1eadp, tuy\u1ec3n ch\u1ecdn VSV ph\u00e2n gi\u1ea3i l\u00e2n c\u00f3 hi\u1ec7u l\u1ef1c cao v\u00e0 \u0111\u1eb7c \u0111i\u1ec3m sinh h\u1ecdc c\u1ee7a ch\u00fang \u0111\u1ec3 s\u1ea3n xu\u1ea5t ph\u00e2n vi sinh cho c\u00e2y l\u00e2m nghi\u1ec7p. T\u1ea1p ch\u00ed Khoa h\u1ecdc L\u00e2m nghi\u1ec7p, s\u1ed1 3: 1038-1045.<\/p>\n

Ph\u1ea1m Quang Thu (1999) \u1ee8ng d\u1ee5ng n\u1ea5m c\u1ed9ng sinh \u0111\u1ec3 s\u1ea3n xu\u1ea5t c\u00e2y con \u1edf V\u01b0\u1eddn \u01b0\u01a1m, T\u1ea1p ch\u00ed c\u00f4ng ngh\u1ec7 th\u1ef1c ph\u1ea9m v\u00e0 N\u00f4ng nghi\u1ec7p, S\u1ed1 9 (1999), trang (414-415).<\/p>\n

Ph\u1ea1m Quang Thu v\u00e0 \u0110\u1eb7ng Nh\u01b0 Qu\u1ef3nh (2007) Th\u00e0nh ph\u1ea7n lo\u00e0i n\u1ea5m ngo\u1ea1i c\u1ed9ng sinh v\u1edbi b\u1ea1ch \u0111\u00e0n v\u00e0 th\u00f4ng. T\u1ea1p ch\u00ed N\u00f4ng nghi\u1ec7p v\u00e0 Ph\u00e1t tri\u1ec3n N\u00f4ng th\u00f4n, s\u1ed1 18: 73-80.<\/p>\n

Ph\u1ea1m Quang Thu v\u00e0 \u0110\u1eb7ng Nh\u01b0 Qu\u1ef3nh (2008) \u0110\u1eb7c \u0111i\u1ec3m sinh tr\u01b0\u1edfng c\u1ee7a h\u1ec7 s\u1ee3i v\u00e0 s\u1ef1 h\u00ecnh th\u00e0nh r\u1ec5 n\u1ea5m c\u1ee7a m\u1ed9t s\u1ed1 lo\u00e0i n\u1ea5m ngo\u1ea1i c\u1ed9ng sinh v\u1edbi b\u1ea1ch \u0111\u00e0n trong nu\u00f4i c\u1ea5y thu\u1ea7n khi\u1ebft. T\u1ea1p ch\u00ed N\u00f4ng nghi\u1ec7p v\u00e0 Ph\u00e1t tri\u1ec3n N\u00f4ng th\u00f4n, s\u1ed1 9: 84-90.<\/p>\n

Ph\u1ea1m Quang Thu v\u00e0 Nguy\u1ec5n Th\u1ecb Th\u00fay Nga (2007) Ph\u00e2n l\u1eadp v\u00e0 tuy\u1ec3n ch\u1ecdn VK n\u1ed9i sinh \u0111\u1ec3 ph\u00f2ng tr\u1eeb n\u1ea5m Cryptosporiopsis eucalypti<\/i> Sankaran & Shutton g\u00e2y b\u1ec7nh ch\u00e1y l\u00e1 b\u1ea1ch \u0111\u00e0n. T\u1ea1p ch\u00ed Khoa h\u1ecdc L\u00e2m nghi\u1ec7p, s\u1ed1 4: 479-485.<\/p>\n

Ph\u1ea1m Quang Thu, Tr\u1ea7n Thanh Tr\u0103ng, Nguy\u1ec5n Ho\u00e0i Thu, Nguy\u1ec5n M\u1ea1nh H\u00e0 v\u00e0 \u0110\u1eb7ng Nh\u01b0 Qu\u1ef3nh (2009) \u1ee8ng d\u1ee5ng ch\u1ebf ph\u1ea9m vi\u00ean n\u00e9n vi sinh h\u1ed7n h\u1ee3p MF1 cho c\u00e2y th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n \u1edf v\u01b0\u1eddn \u01b0\u01a1m. K\u1ef7 y\u1ebfu H\u1ed9i ngh\u1ecb khoa h\u1ecdc c\u00f4ng ngh\u1ec7 l\u00e2m nghi\u1ec7p khu v\u1ef1c ph\u00eda b\u1eafc, th\u00e1ng 10 n\u0103m 2009, trang 517-525.<\/p>\n

Tr\u1ea7n Thanh Tr\u0103ng (2009) \u0110\u1ecbnh lo\u1ea1i VSV ph\u00e2n gi\u1ea3i l\u00e2n v\u00e0 VSV \u0111\u1ed1i kh\u00e1ng n\u1ea5m b\u1ec7nh b\u1ea1ch \u0111\u00e0n v\u00e0 th\u00f4ng b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p sinh h\u1ecdc ph\u00e2n t\u1eed. T\u1ea1p ch\u00ed Khoa h\u1ecdc L\u00e2m nghi\u1ec7p, s\u1ed1 3: 1032-1037.<\/p>\n","protected":false},"excerpt":{"rendered":"

PGS. TS. Ph\u1ea1m Quang Thu Vi\u1ec7n Khoa h\u1ecdc L\u00e2m nghi\u1ec7p Vi\u1ec7t Nam 1. \u0110\u1eb6T V\u1ea4N \u0110\u1ec0 C\u00e2y th\u00f4ng v\u00e0 c\u00e2y b\u1ea1ch \u0111\u00e0n \u0111ang l\u00e0 nh\u1eefng lo\u00e0i c\u00e2y tr\u1ed3ng r\u1eebng ph\u1ed5 bi\u1ebfn \u1edf n\u01b0\u1edbc ta. C\u1ea3 th\u00f4ng v\u00e0 b\u1ea1ch \u0111\u00e0n \u0111\u1ec1u mang l\u1ea1i c\u00e1c gi\u00e1 tr\u1ecb v\u1ec1 kinh t\u1ebf v\u00e0 m\u00f4i tr\u01b0\u1eddng cho ng\u01b0\u1eddi tr\u1ed3ng r\u1eebng. […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[13,72],"tags":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/14435"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=14435"}],"version-history":[{"count":4,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/14435\/revisions"}],"predecessor-version":[{"id":19184,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/14435\/revisions\/19184"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=14435"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/categories?post=14435"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/tags?post=14435"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}