{"id":13678,"date":"2014-10-13T08:52:35","date_gmt":"2014-10-13T01:52:35","guid":{"rendered":"http:\/\/vafs.gov.vn\/vn\/?p=13678"},"modified":"2014-08-29T15:02:15","modified_gmt":"2014-08-29T08:02:15","slug":"bao-cao-ket-qua-de-tai-nhan-nhanh-giong-keo-lai-tu-nhien-keo-lai-nhan-tao-bach-dan-uro-bach-dan-lai-nhan-tao-la-lat-hoa-bang-cong-nghe-bao","status":"publish","type":"post","link":"https:\/\/vafs.gov.vn\/vn\/bao-cao-ket-qua-de-tai-nhan-nhanh-giong-keo-lai-tu-nhien-keo-lai-nhan-tao-bach-dan-uro-bach-dan-lai-nhan-tao-la-lat-hoa-bang-cong-nghe-bao\/","title":{"rendered":"B\u00e1o c\u00e1o k\u1ebft qu\u1ea3 \u0111\u1ec1 t\u00e0i: Nh\u00e2n nhanh gi\u1ed1ng keo lai t\u1ef1 nhi\u00ean, keo lai nh\u00e2n t\u1ea1o, b\u1ea1ch \u0111\u00e0n Uro, b\u1ea1ch \u0111\u00e0n l\u1ea1i nh\u00e2n t\u1ea1o l\u00e0 l\u00e1t hoa b\u1eb1ng c\u00f4ng ngh\u1ec7 b\u00e0o"},"content":{"rendered":"
    \n
  1. \u0110\u1eb6T V\u1ea4N \u0110\u1ec0<\/b><\/li>\n<\/ol>\n

    Nh\u1eefng n\u0103m g\u1ea7n \u0111\u00e2y, vi\u1ec7c \u1ee9ng d\u1ee5ng ti\u1ebfn b\u1ed9 k\u1ef9 thu\u1eadt v\u1ec1 c\u00f4ng ngh\u1ec7 t\u1ebf b\u00e0o th\u1ef1c v\u1eadt trong c\u00f4ng t\u00e1c ch\u1ecdn gi\u1ed1ng c\u00e2y r\u1eebng \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n t\u1ea1i nhi\u1ec1u n\u01b0\u1edbc tr\u00ean th\u1ebf gi\u1edbi. \u1ede Vi\u1ec7t Nam nu\u00f4i c\u1ea5y m\u00f4 \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n t\u1eeb nh\u1eefng n\u0103m 70. Tuy nhi\u00ean, c\u00e1c \u1ee9ng d\u1ee5ng v\u1ec1 nu\u00f4i c\u1ea5y m\u00f4 trong l\u00e2m nghi\u1ec7p c\u00f2n nhi\u1ec1u h\u1ea1n ch\u1ebf v\u1ec1 k\u1ef9 thu\u1eadt, quy m\u00f4, v\u1ed1n \u0111\u1ea7u t\u01b0, d\u1eabn \u0111\u1ebfn \u00a0gi\u00e1 th\u00e0nh c\u00e2y con t\u1eeb nu\u00f4i c\u1ea5y m\u00f4 cao h\u01a1n nhi\u1ec1u so v\u1edbi c\u00e2y hom v\u00e0 c\u00e2y h\u1ea1t do \u0111\u00f3 di\u1ec7n t\u00edch r\u1eebng tr\u1ed3ng t\u1eeb c\u00e2y m\u00f4 ch\u01b0a nhi\u1ec1u. M\u1eb7c d\u00f9 v\u1eady, c\u00e2y m\u00f4 v\u1edbi c\u00e1c \u01b0u \u0111i\u1ec3m v\u01b0\u1ee3t tr\u1ed9i nh\u01b0: c\u00f3 th\u1ec3 s\u1ea3n xu\u1ea5t quanh n\u0103m kh\u00f4ng ph\u1ee5 thu\u1ed9c v\u00e0o m\u00f9a v\u1ee5, c\u1ea7n \u00edt di\u1ec7n t\u00edch s\u1ea3n xu\u1ea5t, c\u00e2y gi\u1ed1ng s\u1ea3n xu\u1ea5t ra ho\u00e0n to\u00e0n s\u1ea1ch b\u1ec7nh, \u0111\u1ed3ng nh\u1ea5t v\u1ec1 m\u1eb7t di truy\u1ec1n, c\u00f3 \u0111\u1ed9 tr\u1ebb h\u00f3a cao v\u00e0 c\u00f3 b\u1ed9 r\u1ec5 gi\u1ed1ng nh\u01b0 c\u00e2y con t\u1eeb h\u1ea1t n\u00ean c\u00f3 kh\u1ea3 n\u0103ng sinh tr\u01b0\u1edfng v\u00e0 ph\u00e1t tri\u1ec3n t\u1ed1t h\u01a1n tr\u00ean hi\u1ec7n tr\u01b0\u1eddng. N\u00ean trong th\u1eddi gian t\u1edbi c\u00e2y con s\u1ea3n xu\u1ea5t t\u1eeb nu\u00f4i c\u1ea5y m\u00f4 s\u1ebd c\u00f3 v\u1ecb tr\u00ed x\u1ee9ng \u0111\u00e1ng trong s\u1ea3n xu\u1ea5t l\u00e2m nghi\u1ec7p.<\/p>\n

    Trong c\u00e1c nghi\u00ean c\u1ee9u v\u1ec1 ch\u1ecdn gi\u1ed1ng c\u00e2y r\u1eebng, Trung t\u00e2m Nghi\u00ean c\u1ee9u Gi\u1ed1ng c\u00e2y r\u1eebng \u0111\u00e3 ch\u1ecdn t\u1ea1o \u0111\u01b0\u1ee3c m\u1ed9t s\u1ed1 gi\u1ed1ng Keo lai t\u1ef1 nhi\u00ean, Keo lai nh\u00e2n t\u1ea1o, b\u1ea1ch \u0111\u00e0n lai v\u00e0 m\u1ed9t s\u1ed1 xu\u1ea5t x\u1ee9 l\u00e1t hoa c\u00f3 n\u0103ng su\u1ea5t cao (L\u00ea \u0110\u00ecnh Kh\u1ea3 v\u00e0 c\u1ed9ng s\u1ef1 , 2003, H\u00e0 Huy Th\u1ecbnh v\u00e0 c\u1ed9ng s\u1ef1 2006). C\u00e1c gi\u1ed1ng Keo lai v\u00e0 b\u1ea1ch \u0111\u00e0n lai \u0111\u00e3 \u0111\u01b0\u1ee3c c\u00f4ng nh\u1eadn l\u00e0 gi\u1ed1ng ti\u1ebfn b\u1ed9 k\u1ef9 thu\u1eadt b\u1edfi kh\u1ea3 n\u0103ng sinh tr\u01b0\u1edfng t\u1ed1t tr\u00ean nhi\u1ec1u d\u1ea1ng l\u1eadp \u0111\u1ecba. Vi\u1ec7c ph\u00e1t tri\u1ec3n nhanh c\u00e1c gi\u1ed1ng n\u00e0y v\u00e0o trong s\u1ea3n xu\u1ea5t l\u00e2m nghi\u1ec7p l\u00e0 m\u1ed9t vi\u1ec7c l\u00e0m c\u00f3 \u00fd ngh\u0129a, ngo\u00e0i vi\u1ec7c n\u00e2ng cao n\u0103ng su\u1ea5t r\u1eebng tr\u1ed3ng n\u00f3 c\u00f2n l\u00e0m t\u0103ng t\u00ednh an to\u00e0n sinh h\u1ecdc trong tr\u1ed3ng r\u1eebng v\u00f4 t\u00ednh. \u0110\u1ec3 l\u00e0m \u0111\u01b0\u1ee3c c\u00f4ng t\u00e1c ph\u00e1t tri\u1ec3n gi\u1ed1ng n\u00e0y, ho\u00e0n thi\u1ec7n c\u00f4ng ngh\u1ec7 nh\u00e2n gi\u1ed1ng b\u1eb1ng nu\u00f4i c\u1ea5y m\u00f4 v\u1edbi h\u1ec7 s\u1ed1 nh\u00e2n gi\u1ed1ng cao, th\u1eddi gian nh\u00e2n gi\u1ed1ng \u0111\u01b0\u1ee3c r\u00fat ng\u1eafn v\u00e0 h\u1ea1 gi\u00e1 th\u00e0nh s\u1ea3n xu\u1ea5t c\u00e2y con l\u00e0 vi\u1ec7c l\u00e0m h\u1ebft s\u1ee9c c\u00f3 \u00fd ngh\u0129a.<\/p>\n

    II. M\u1ee4C TI\u00caU NGHI\u00caN C\u1ee8U<\/b><\/p>\n

    Nh\u00e2n nhanh m\u1ed9t s\u1ed1 gi\u1ed1ng c\u00e2y l\u00e2m nghi\u1ec7p m\u1edbi ch\u1ecdn t\u1ea1o b\u1eb1ng c\u00f4ng ngh\u1ec7 t\u1ebf b\u00e0o<\/p>\n

    \"Nhan<\/a><\/p>\n

    III. \u0110\u1ed0I T\u01af\u1ee2NG, V\u1eacT LI\u1ec6U V\u00c0 PH\u01af\u01a0NG PH\u00c1P NGHI\u00caN C\u1ee8U<\/b><\/p>\n

    III.1. \u0110\u1ed1i t\u01b0\u1ee3ng nghi\u00ean c\u1ee9u<\/b><\/p>\n

    + Keo lai t\u1ef1 nhi\u00ean: <\/i>gi\u1ed1ng BV71, BV73, BV75.<\/p>\n

    + Keo lai nh\u00e2n t\u1ea1o gi\u1ed1ng AM2<\/p>\n

    + B\u1ea1ch \u0111\u00e0n lai nh\u00e2n t\u1ea1o gi\u1ed1ng UE35<\/p>\n

    + L\u00e1t hoa (Ngh\u1ec7 An v\u00e0 Th\u00e1i Lan)<\/b><\/p>\n

    III.2. V\u1eadt li\u1ec7u nghi\u00ean c\u1ee9u<\/b><\/p>\n

    V\u1eadt li\u1ec7u d\u00f9ng trong nu\u00f4i c\u1ea5y l\u00e0 ch\u1ed3i l\u1ea5y t\u1eeb c\u00e2y v\u1eadt li\u1ec7u g\u1ed1c 1 n\u0103m tu\u1ed5i t\u1ea1i v\u01b0\u1eddn \u01b0\u01a1m c\u1ee7a Trung t\u00e2m nghi\u00ean c\u1ee9u gi\u1ed1ng c\u00e2y r\u1eebng c\u1ee7a c\u00e1c gi\u1ed1ng c\u00e2y l\u00e2m nghi\u1ec7p m\u1edbi ch\u1ecdn l\u1ecdc<\/p>\n

    III.3. Ph\u01b0\u01a1ng ph\u00e1p nghi\u00ean c\u1ee9u<\/b><\/p>\n

    III.3.1. Kh\u1eed tr\u00f9ng m\u1eabu v\u1eadt<\/b><\/p>\n

    Qu\u00e1 tr\u00ecnh kh\u1eed tr\u00f9ng \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh theo ph\u01b0\u01a1ng ph\u00e1p kh\u1eed tr\u00f9ng cho m\u1ed9t s\u1ed1 lo\u00e0i c\u00e2y r\u1eebng (\u0110o\u00e0n Th\u1ecb Mai v\u00e0 c\u1ed9ng s\u1ef1 2000, 2003, 2005, 2009a v\u00e0 2009b).<\/p>\n

    III.3.2. M\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y<\/b><\/p>\n

    L\u00e0 m\u00f4i tr\u01b0\u1eddng Murashige & Skoog (MS) \u0111\u1ed1i v\u1edbi keo lai v\u00e0 b\u1ea1ch \u0111\u00e0n lai, v\u00e0 WPM (wooden plant medium) (k\u00ed hi\u1ec7u WPM) \u0111\u1ed1i v\u1edbi l\u00e1t hoa, c\u00e1c m\u00f4i tr\u01b0\u1eddng \u0111\u01b0\u1ee3c b\u1ed5 sung m\u1ed9t s\u1ed1 ch\u1ea5t \u0111i\u1ec1u h\u00f2a sinh tr\u01b0\u1edfng thu\u1ed9c hai nh\u00f3m Auxin, Cytokinin, ch\u1ea5t ph\u1ee5 gia, c\u00e1c vitamin, \u0111\u01b0\u1eddng. \u0110\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh PH = 5,8, th\u1eddi gian h\u1ea5p kh\u1eed tr\u00f9ng 20 ph\u00fat (1.2 atm t\u1ea1i 1210<\/sup>C)<\/p>\n

    III.3.3. \u0110i\u1ec1u ki\u1ec7n nu\u00f4i c\u1ea5y<\/b><\/p>\n

    + S\u1ed1 gi\u1edd chi\u1ebfu s\u00e1ng trong ng\u00e0y l\u00e0 10h\/ng\u00e0y.<\/p>\n

    + C\u01b0\u1eddng \u0111\u1ed9 \u00e1nh s\u00e1ng kho\u1ea3ng 2000-3000 Lux.<\/p>\n

    + Nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng nu\u00f4i 25 – 27o<\/sup>C \u0111\u1ed1i v\u1edbi Keo, 27- 290<\/sup>C cho B\u1ea1ch \u0111\u00e0n<\/p>\n

    III.3.4. X\u1eed l\u00fd s\u1ed1 li\u1ec7u<\/b><\/p>\n

    C\u00e1c th\u00ed nghi\u1ec7m \u0111\u1ec1u \u0111\u01b0\u1ee3c b\u1ed1 tr\u00ed nhi\u1ec1u l\u1ea7n l\u1eb7p v\u00e0 x\u1eed l\u00fd k\u1ebft qu\u1ea3 theo ph\u01b0\u01a1ng ph\u00e1p th\u1ed1ng k\u00ea th\u00f4ng th\u01b0\u1eddng tr\u00ean m\u00e1y t\u00ednh b\u1eb1ng ph\u1ea7n m\u1ec1m EXCEL.<\/p>\n

      \n
    1. IV.\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/b>K\u1ebeT QU\u1ea2 NGHI\u00caN C\u1ee8U<\/b><\/li>\n<\/ol>\n

      IV.1. Kh\u1eed tr\u00f9ng<\/b><\/p>\n

      \u0110\u1ec3 x\u00e1c \u0111\u1ecbnh ph\u01b0\u01a1ng ph\u00e1p kh\u1eed tr\u00f9ng t\u1ed1t nh\u1ea5t cho t\u1eebng \u0111\u1ed1i t\u01b0\u1ee3ng nghi\u00ean c\u1ee9u, hai lo\u1ea1i h\u00f3a ch\u1ea5t th\u00f4ng d\u1ee5ng th\u01b0\u1eddng \u0111\u01b0\u1ee3c d\u00f9ng trong nu\u00f4i c\u1ea5y m\u00f4 l\u00e0 HgCl2<\/sub> v\u00e0 canxihypoclorit (Ca(OCl)2<\/sub>) \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1eed nghi\u1ec7m v\u1edbi c\u00e1c lo\u1ea1i n\u1ed3ng \u0111\u1ed9 (0,1%, 0,05% v\u1edbi HgCl2<\/sub>, 5% v\u00e0 10% v\u1edbi Ca(OCl)2<\/sub> v\u00e0 kho\u1ea3ng th\u1eddi gian kh\u1eed tr\u00f9ng kh\u00e1c nhau (2, 4, 6, 8, 10, 12, 14, 16, 18, 20 ph\u00fat). K\u1ebft qu\u1ea3 \u0111i\u1ec1u ki\u1ec7n kh\u1eed tr\u00f9ng th\u00edch h\u1ee3p nh\u1ea5t cho t\u1eebng lo\u1ea1i c\u00e2y \u0111\u01b0\u1ee3c t\u1ed5ng h\u1ee3p trong b\u1ea3ng 1 sau \u0111\u00e2y.<\/p>\n

      B\u1ea3ng 1. K\u1ebft qu\u1ea3 \u0111i\u1ec1u ki\u1ec7n kh\u1eed tr\u00f9ng th\u00edch h\u1ee3p nh\u1ea5t cho c\u00e1c c\u00e2y th\u00ed nghi\u1ec7m.<\/i><\/p>\n

       <\/p>\n\n\n\n\n\n\n
      \n

      \u0110\u1ed1i t\u01b0\u1ee3ng nghi\u00ean c\u1ee9u<\/b><\/b><\/p>\n<\/td>\n

      		\n

      H\u00f3a ch\u1ea5t kh\u1eed tr\u00f9ng<\/b><\/b><\/p>\n<\/td>\n

      		\n

      Th\u1eddi gian kh\u1eed tr\u00f9ng (ph\u00fat)<\/b><\/b><\/p>\n<\/td>\n

      		\n

      T\u1ef7 l\u1ec7 b\u1eadt ch\u1ed3i (%)<\/b><\/b><\/p>\n<\/td>\n

      		\n

      Sai d\u1ecb (Sd)<\/b><\/b><\/p>\n<\/td>\n<\/tr>\n

      Keo lai (BV71, BV73, BV75, AM2)<\/td>\n\n

      HgCl2<\/sub> 0,5%<\/p>\n<\/td>\n

      		\n

      8 – 10<\/p>\n<\/td>\n

      		\n

      12,6 – 15,6<\/p>\n<\/td>\n

      		\n

      1,3-2,2<\/p>\n<\/td>\n<\/tr>\n

      L\u00e1t hoa (L\u00e1t Ngh\u1ec7 An, L\u00e1t Th\u00e1i Lan)<\/td>\n\n

      HgCl2 0,1%<\/p>\n<\/td>\n

      		\n

      14<\/p>\n<\/td>\n

      		\n

      20,4<\/p>\n<\/td>\n

      		\n

      1,45<\/p>\n<\/td>\n<\/tr>\n

      B\u1ea1ch \u0111\u00e0n (UE35, PN3D)<\/td>\n\n

      HgCl2 0,1%<\/p>\n<\/td>\n

      		\n

      10 – 12<\/p>\n<\/td>\n

      		\n

      15,6<\/p>\n<\/td>\n

      		\n

      2,3<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      V\u1edbi b\u1ea1ch \u0111\u00e0n lai Uro, ph\u01b0\u01a1ng ph\u00e1p kh\u1eed tr\u00f9ng th\u00edch h\u1ee3p l\u00e0 s\u1eed d\u1ee5ng dung d\u1ecbch HgCl2<\/sub> v\u1edbi n\u1ed3ng \u0111\u1ed9 0,1% v\u00e0 m\u1eabu v\u1eadt \u0111\u01b0\u1ee3c kh\u1eed tr\u00f9ng trong th\u1eddi gian 12 ph\u00fat cho k\u1ebft qu\u1ea3 kh\u1eed tr\u00f9ng cao nh\u1ea5t. Khi \u00e1p d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p n\u00e0y, t\u1ef7 l\u1ec7 m\u1eabu nhi\u1ec5m trung b\u00ecnh m\u1eabu l\u00e0 50,2% trong khi t\u1ef7 l\u1ec7 m\u1eabu b\u1eadt ch\u1ed3i \u0111\u1ea1t t\u1edbi 17,5%.<\/p>\n

      K\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m cho th\u1ea5y \u0111\u1ed1i v\u1edbi L\u00e1t hoa kh\u1eed tr\u00f9ng b\u1eb1ng HgCl2 <\/sub>0,1%\u00a0 trong 15 ph\u00fat cho hi\u1ec7u qu\u1ea3 t\u1ed1t nh\u1ea5t, v\u1edbi t\u1ef7 l\u1ec7 m\u1eabu s\u1ea1ch \u0111\u1ea1t 60,37% v\u00e0 t\u1ef7 l\u1ec7 m\u1eabu b\u1eadt ch\u1ed3i cao \u0111\u1ea1t 20,37%.<\/p>\n

      V\u1edbi c\u00e1c d\u00f2ng keo lai nghi\u00ean c\u1ee9u, vi\u1ec7c s\u1eed d\u1ee5ng HgCl2<\/sub> trong kho\u1ea3ng th\u1eddi gian t\u1eeb 8 \u0111\u1ebfn 10 ph\u00fat l\u00e0 th\u00edch h\u1ee3p nh\u1ea5t. M\u1eabu v\u1eadt \u0111\u01b0\u1ee3c kh\u1eed tr\u00f9ng theo ph\u01b0\u01a1ng ph\u00e1p n\u00e0y c\u00f3 t\u1ef7 l\u1ec7 m\u1eabu nhi\u1ec5m th\u1ea5p (ch\u1ec9 t\u1eeb 58,52 \u0111\u1ebfn 72,59%) v\u00e0 t\u1ef7 l\u1ec7 b\u1eadt ch\u1ed3i kh\u00e1 cao (\u0111\u1ea1t t\u1eeb 8,15 \u0111\u1ebfn 11,11%).<\/p>\n

      IV.2. Nh\u00e2n ch\u1ed3i<\/b><\/p>\n

      IV.2.1. X\u00e1c \u0111\u1ecbnh m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y c\u01a1 b\u1ea3n<\/i><\/b><\/p>\n

      C\u00e1c th\u00ed nghi\u1ec7m x\u00e1c \u0111\u1ecbnh m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y c\u01a1 b\u1ea3n cho c\u00e1c \u0111\u1ed1i t\u01b0\u1ee3ng nghi\u00ean c\u1ee9u \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh tr\u00ean 5 lo\u1ea1i m\u00f4i tr\u01b0\u1eddng: Gamborg (B5), McCown Woody plant (WPM), Murashige & Skoog (MS), Schenk & Hildebrandt (SH) v\u00e0 Litvay (L), v\u1edbi 3 l\u1ea7n l\u1eb7p, m\u1ed1i l\u1ea7n th\u00ed nghi\u1ec7m \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh v\u1edbi 3 b\u00ecnh nu\u00f4i c\u1ea5y, m\u1ed7i b\u00ecnh th\u00ed nghi\u1ec7m 15 m\u1eabu.<\/p>\n

      K\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m cho th\u1ea5y m\u00f4i tr\u01b0\u1eddng c\u01a1 b\u1ea3n th\u00edch h\u1ee3p cho c\u00e1c th\u00ed nghi\u1ec7m nu\u00f4i c\u1ea5y m\u00f4 \u0111\u1ed1i v\u1edbi keo lai t\u1ef1 nhi\u00ean (BV71, BV73, BV75), keo lai nh\u00e2n t\u1ea1o (MA2) v\u00e0 B\u1ea1ch \u0111\u00e0n (UE35, PN3D) l\u00e0 m\u00f4i tr\u01b0\u1eddng MS. Ri\u00eang v\u1edbi c\u1ea5c \u0111\u1ed1i t\u01b0\u1ee3ng l\u00e1t hoa nghi\u00ean c\u1ee9u, m\u00f4i tr\u01b0\u1eddng c\u01a1 b\u1ea3n th\u00edch h\u1ee3p cho c\u00e1c th\u00ed nghi\u1ec7m nu\u00f4i c\u1ea5y m\u00f4 l\u00e0 m\u00f4i tr\u01b0\u1eddng WPM.<\/p>\n

      IV.2.2. \u1ea2nh h\u01b0\u1edfng c\u1ee7a c\u00e1c ch\u1ea5t \u0111i\u1ec1u h\u00f2a sinh tr\u01b0\u1edfng \u0111\u1ebfn kh\u1ea3 n\u0103ng nh\u00e2n ch\u1ed3i c\u1ee7a c\u00e1c \u0111\u1ed1i t\u01b0\u1ee3ng nghi\u00ean c\u1ee9u<\/i><\/b><\/p>\n

      Sau kho\u1ea3ng 10- 15 ng\u00e0y c\u1ea5y c\u00e1c ch\u1ed3i b\u1ea5t \u0111\u1ecbnh xu\u1ea5t hi\u1ec7n, khi chi\u1ec1u cao ch\u1ed3i \u0111\u1ea1t 1,5 \u2013 2 cm ch\u1ed3i \u0111\u01b0\u1ee3c c\u1ea5y t\u00e1ch ra. Ch\u1ed3i \u0111\u01b0\u1ee3c c\u1ea5y v\u00e0o m\u00f4i tr\u01b0\u1eddng MS c\u1ea3i ti\u1ebfn (k\u00ed hi\u1ec7u l\u00e0 MS*) c\u00f3 b\u1ed5 sung BAP ho\u1eb7c Kinetin \u1edf c\u00e1c n\u1ed3ng \u0111\u1ed9 kh\u00e1c nhau t\u1eeb 1,0 mg\/l \u0111\u1ebfn 2,0 mg\/l.<\/p>\n

      K\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m thu \u0111\u01b0\u1ee3c cho th\u1ea5y:<\/p>\n

      –\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0110\u1ed1i v\u1edbi Keo lai: k\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m cho th\u1ea5y, BAP c\u00f3 t\u00e1c d\u1ee5ng r\u00f5 l\u00ean qu\u00e1 tr\u00ecnh t\u1ea1o ch\u1ed3i c\u1ee7a c\u00e1c d\u00f2ng Keo lai th\u00ed nghi\u1ec7m so v\u1edbi c\u00f4ng th\u1ee9c \u0111\u1ed1i ch\u1ee9ng hay khi s\u1eed d\u1ee5ng ch\u1ea5t k\u00edch th\u00edch sinh tr\u01b0\u1edfng l\u00e0 Kn \u1edf c\u00f9ng n\u1ed3ng \u0111\u1ed9. K\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m c\u0169ng cho th\u1ea5y, khi b\u1ed5 sung BAP v\u1edbi n\u1ed3ng \u0111\u1ed9 1,5mg\/l v\u00e0o m\u00f4i tr\u01b0\u1eddng nu\u00f4i c\u1ea5y, s\u1ed1 l\u01b0\u1ee3ng ch\u1ed3i thu \u0111\u01b0\u1ee3c sau qu\u00e1 tr\u00ecnh k\u00edch th\u00edch t\u1ea1o ch\u1ed3i \u0111\u1ea1t t\u1eeb 7,5 \u0111\u1ebfn 8,2 ch\u1ed3i\/c\u1ee5m tu\u1ef3 thu\u1ed9c v\u00e0o c\u00e1c d\u00f2ng th\u00ed nghi\u1ec7m. Khi s\u1eed d\u1ee5ng c\u00f4ng th\u1ee9c nh\u00e2n ch\u1ed3i n\u00e0y, c\u00e1c ch\u1ed3i t\u1ea1o \u0111\u01b0\u1ee3c c\u0169ng c\u00f3 chi\u1ec1u cao trung b\u00ecnh cao h\u01a1n so v\u1edbi c\u00e1c c\u00f4ng th\u1ee9c c\u00f2n l\u1ea1i. M\u00f4i tr\u01b0\u1eddng \u0111\u01b0\u1ee3c b\u1ed5 sung BAP v\u1edbi n\u1ed3ng \u0111\u1ed9 2,0mg\/l c\u0169ng cho h\u1ec7 s\u1ed1 nh\u00e2n ch\u1ed3i t\u01b0\u01a1ng \u0111\u1ed1i cao (\u0111\u1ea1t t\u1eeb 7,6 \u0111\u1ebfn 7,7 ch\u1ed3i\/c\u1ee5m) tuy nhi\u00ean chi\u1ec1u cao ch\u1ed3i thu \u0111\u01b0\u1ee3c ch\u1ec9 \u0111\u1ea1t trung b\u00ecnh t\u1eeb 4,1 \u0111\u1ebfn 4,3 cm (ch\u1ec9 s\u1ed1 n\u00e0y \u0111\u1ea1t 4,4 \u0111\u1ebfn 4,6cm khi s\u1eed d\u1ee5ng BAP n\u1ed3ng \u0111\u1ed9 1,5mg\/l).<\/p>\n

      –\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u0110\u1ed1i v\u1edbi hai \u0111\u1ed1i t\u01b0\u1ee3ng b\u1ea1ch \u0111\u00e0n nghi\u00ean c\u1ee9u, m\u00f4i tr\u01b0\u1eddng th\u00edch h\u1ee3p cho qu\u00e1 tr\u00ecnh nh\u00e2n ch\u1ed3i l\u00e0 m\u00f4i tr\u01b0\u1eddng MS* (m\u00f4i tr\u01b0\u1eddng MS \u0111\u00e3 \u0111\u01b0\u1ee3c thay \u0111\u1ed5i v\u1ec1 th\u00e0nh ph\u1ea7n v\u00e0 n\u1ed3ng \u0111\u1ed9 c\u00e1c ch\u1ea5t kho\u00e1ng, vi tamin) c\u00f3 b\u1ed5 sung BAP v\u1edbi n\u1ed3ng \u0111\u1ed9 0,5 mg\/l. Khi \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y trong m\u00f4i tr\u01b0\u1eddng n\u00e0y, c\u00e1c d\u00f2ng B\u1ea1ch \u0111\u00e0n nu\u00f4i c\u1ea5y cho h\u1ec7 s\u1ed1 nh\u00e2n ch\u1ed3i \u0111\u1ea1t t\u1eeb 6,8 \u0111\u1ebfn 7,5 v\u1edbi chi\u1ec1u cao ch\u1ed3i trung b\u00ecnh \u0111\u1ea1t t\u1eeb 2,4 \u0111\u1ebfn 2,5 cm.<\/p>\n

      –\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 K\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m x\u00e1c \u0111\u1ecbnh m\u00f4i tr\u01b0\u1eddng nh\u00e2n ch\u1ed3i cho L\u00e1t hoa cho th\u1ea5y, c\u0169ng t\u01b0\u01a1ng t\u1ef1 nh\u01b0 v\u1edbi s\u1ed1 \u0111\u1ed1i t\u01b0\u1ee3ng c\u00e2y r\u1eebng kh\u00e1c, BAP c\u00f3 \u1ea3nh h\u01b0\u1edfng r\u00f5 r\u1ec7t \u0111\u1ebfn qu\u00e1 tr\u00ecnh t\u1ea1o ch\u1ed3i cu\u1ea3 c\u00e1c m\u1eabu v\u1eadt nu\u00f4i c\u1ea5y, kh\u1ea3 n\u0103ng k\u00edch th\u00edch t\u1ea1o ch\u1ed3i c\u1ee7a BAP l\u00e0 cao h\u01a1n h\u1eb3n Kn khi s\u1eed d\u1ee5ng c\u00f9ng n\u1ed3ng \u0111\u1ed9 trong m\u00f4i tr\u01b0\u1eddng nh\u00e2n ch\u1ed3i. V\u1edbi c\u1ea3 2 \u0111\u1ed1i t\u01b0\u1ee3ng L\u00e1t hoa nghi\u00ean c\u1ee9u, m\u00f4i tr\u01b0\u1eddng nh\u00e2n ch\u1ed3i th\u00edch h\u1ee3p l\u00e0 m\u00f4i tr\u01b0\u1eddng c\u00f3 th\u00e0nh ph\u1ea7n kho\u00e1ng c\u01a1 b\u1ea3n l\u00e0 m\u00f4i tr\u01b0\u1eddng WPM \u0111\u00e3 \u0111\u01b0\u1ee3c c\u00e1i ti\u1ebfn b\u1eb1ng vi\u1ec7c thay \u0111\u1ed5i n\u1ed3ng \u0111\u1ed9 c\u00e1c ch\u1ea5t kho\u00e1ng \u0111a l\u01b0\u1ee3ng, vi l\u01b0\u1ee3ng v\u00e0 m\u1ed9t s\u1ed1 vitamin v\u00e0 axit amin (k\u00ed hi\u1ec7u WPM*) c\u00f3 b\u1ed5 sung BAP n\u1ed3ng \u0111\u1ed9 0,75mg\/l.<\/p>\n

      IV.3. Ra r\u1ec5<\/b><\/p>\n

      T\u1ea1o r\u1ec5 v\u00e0 hu\u1ea5n luy\u1ec7n l\u00e0 giai \u0111o\u1ea1n chuy\u1ec3n ch\u1ed3i t\u1eeb m\u00f4i tr\u01b0\u1eddng nh\u00e2n nhanh sang m\u00f4i tr\u01b0\u1eddng t\u1ea1o r\u1ec5 \u0111\u1ec3 c\u00f3 \u0111\u01b0\u1ee3c c\u00e2y con ho\u00e0n ch\u1ec9nh ho\u1eb7c c\u00f3 th\u1ec3 cho ra r\u1ec5 tr\u1ef1c ti\u1ebfp tr\u00ean n\u1ec1n c\u00e1t trong nh\u00e0 k\u00ednh. Ch\u1ecdn c\u00e1c ch\u1ed3i \u0111\u1ee7 ti\u00eau chu\u1ea9n v\u1ec1 chi\u1ec1u cao, ch\u1ea5t l\u01b0\u1ee3ng nu\u00f4i c\u1ea5y tr\u00ean c\u00e1c m\u00f4i tr\u01b0\u1eddng t\u1ea1o r\u1ec5 nh\u1eb1m x\u00e1c \u0111\u1ecbnh m\u00f4i tr\u01b0\u1eddng th\u00edch h\u1ee3p \u0111\u1ec3 t\u1ea1o c\u00e2y con ho\u00e0n ch\u1ec9nh.<\/p>\n

      Khi ch\u1ed3i \u0111\u1ea1t chi\u1ec1u cao 3 – 4cm \u0111\u1ed1i v\u1edbi Keo lai t\u1ef1 nhi\u00ean, 2,7 \u2013 3cm \u0111\u1ed1i v\u1edbi B\u1ea1ch \u0111\u00e0n v\u00e0 l\u00e1t hoa c\u00f3 th\u1ec3 c\u1eaft \u0111\u1ec3 c\u1ea5y chuy\u1ec3n sang m\u00f4i tr\u01b0\u1eddng h\u00ecnh th\u00e0nh r\u1ec5 l\u00e0 m\u00f4i tr\u01b0\u1eddng 1\/2MS*<\/sup> c\u00f3 b\u1ed5 xung IBA (Axit \u03b2 \u2013 indol butyric), NAA (Axit \u03b1 \u2013 naphtylacetic). \u00a0K\u1ebft qu\u1ea3 t\u1ed5ng h\u1ee3p \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y trong b\u1ea3ng sau:<\/p>\n

      B\u1ea3ng 2. T\u1ed5ng h\u1ee3p k\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m ra r\u1ec5 trong ph\u00f2ng th\u00ed nghi\u1ec7m cho c\u00e1c \u0111\u1ed1i t\u01b0\u1ee3ng nghi\u00ean c\u1ee9u<\/i><\/p>\n\n\n\n\n\n\n\n
      \u0110\u1ed1i t\u01b0\u1ee3ng nghi\u00ean c\u1ee9u <\/b><\/td>\n\n

      \u00a0<\/b><\/p>\n

      M\u00f4i tr\u01b0\u1eddng<\/b><\/p>\n<\/td>\n

      		\n

      T\u1ef7 l\u1ec7 ra r\u1ec5 (%)<\/b><\/p>\n

      (Tb \u00b1 Sd)<\/b><\/p>\n<\/td>\n

      		\n

      S\u1ed1 r\u1ec5 trung b\u00ecnh (c\u00e1i)<\/b><\/p>\n<\/td>\n

      		\n

      Chi\u1ec1u d\u00e0i r\u1ec5 (cm)<\/b><\/p>\n<\/td>\n<\/tr>\n

      Keo lai t\u1ef1 nhi\u00ean<\/td>\n1\/2MS*<\/sup> + IBA\u00a0 1,5 mg\/l<\/td>\n89,5 \u00b1 4,6<\/td>\n4,5\u00a0 \u00b1 1,4<\/td>\n3,8 \u00b1 1,1<\/td>\n<\/tr>\n
      Keo lai nh\u00e2n t\u1ea1o<\/td>\n1\/2MS*<\/sup> + IBA 1,0 mg\/l<\/td>\n76,3 \u00b1 5,2<\/td>\n5,8\u00a0 \u00b1 2,5<\/td>\n4,3 \u00b1 2,3<\/td>\n<\/tr>\n
      L\u00e1t hoa<\/td>\n1\/2MS*<\/sup> + IBA 1,0 mg\/l<\/td>\n93,3 \u00b1 4,8<\/td>\n4,7\u00a0 \u00b1 2,62<\/td>\n\u00a05,8 \u00b1 2,4<\/td>\n<\/tr>\n
      B\u1ea1ch \u0111\u00e0n<\/td>\n1\/2MS*<\/sup> + IBA 1,5 mg\/l + 0,1 ABT mg\/l<\/td>\n82,2 \u00b1 6,4<\/td>\n1,3 \u00b1 0,8<\/td>\n2,9 \u00b1 0,8<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      K\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m cho th\u1ea5y, m\u00f4i tr\u01b0\u1eddng 1\/2MS* \u0111\u01b0\u1ee3c b\u1ed5 sung 1,5mg\/l IBA l\u00e0 th\u00edch h\u1ee3p nh\u1ea5t cho qu\u00e1 tr\u00ecnh t\u1ea1o r\u1ec5 nh\u00e2n t\u1ea1o c\u1ee7a c\u00e1c d\u00f2ng Keo lai BV71 v\u00e0 BV73. V\u1edbi d\u00f2ng BV75 t\u1ef7 l\u1ec7 ra r\u1ec5 \u0111\u1ea1t 92,45% khi s\u1eed d\u1ee5ng m\u00f4i tr\u01b0\u1eddng 1\/2MS* c\u00f3 b\u1ed5 sung IBA v\u1edbi n\u1ed3ng \u0111\u1ed9 2,0mg\/l, tuy nhi\u00ean n\u1ebfu s\u1eed d\u1ee5ng m\u00f4i tr\u01b0\u1eddng c\u00f3 b\u1ed5 sung IBA v\u1edbi n\u1ed3ng \u0111\u1ed9 1,5mg\/l c\u0169ng cho t\u1ef7 l\u1ec7 ra r\u1ec5 kh\u00e1 cao, \u0111\u1ea1t t\u1edbi 91,06%. M\u1eb7t kh\u00e1c, khi s\u1eed d\u1ee5ng IBA v\u1edbi n\u1ed3ng \u0111\u1ed9 cao, m\u1eb7c d\u00f9 ch\u1ec9 ti\u00eau s\u1ed1 r\u1ec5 trung b\u00ecnh\/ch\u1ed3i \u0111\u1ea1t cao h\u01a1n so v\u1edbi khi s\u1eed d\u1ee5ng ch\u1ea5t k\u00edch th\u00edch t\u1ea1o r\u1ec5 \u1edf n\u1ed3ng \u0111\u1ed9 th\u1ea5p h\u01a1n nh\u01b0ng chi\u1ec1u d\u00e0i r\u1ec5 tr\u00f9ng b\u00ecnh v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng r\u1ec5 thu \u0111\u01b0\u1ee3c l\u1ea1i th\u1ea5p h\u01a1n. Do \u0111\u00f3, c\u00f3 th\u1ec3 k\u1ebft lu\u1eadn v\u1edbi c\u00e1c d\u00f2ng Keo lai th\u00ed nghi\u1ec7m m\u00f4i tr\u01b0\u1eddng t\u1ea1o r\u1ec5 th\u00edch h\u1ee3p l\u00e0 m\u00f4i tr\u01b0\u1eddng 1\/2MS* + IBA v\u1edbi n\u1ed3ng \u0111\u1ed9 1,5mg\/l.<\/p>\n

      \u0110\u1ed1i v\u1edbi hai d\u00f2ng L\u00e1t hoa nghi\u00ean c\u1ee9u IBA c\u00f3 t\u00e1c d\u1ee5ng m\u1ea1nh m\u1ebd \u0111\u1ebfn qu\u00e1 tr\u00ecnh t\u1ea1o r\u1ec5. T\u1ef7 l\u1ec7 ra r\u1ec5 c\u1ee7a c\u1ea3 hai d\u00f2ng L\u00e1t hoa khi c\u00f3 b\u1ed5 sung c\u1ee7a IBA \u0111\u1ea1t kh\u00e1 cao 59,31% – 93,33%, trong khi \u0111\u00f3 s\u1eed d\u1ee5ng NAA \u0111\u1ec3 k\u00edch th\u00edch t\u1ea1o r\u1ec5 th\u00ec t\u1ef7 l\u1ec7 n\u00e0y ch\u1ec9 \u0111\u1ea1t t\u1eeb 48,15% – 66,67%.<\/p>\n

      K\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m cho th\u1ea5y, \u0111\u1ed1i v\u1edbi B\u1ea1ch \u0111\u00e0n, k\u1ebft qu\u1ea3 th\u00ed nghi\u1ec7m k\u00edch th\u00edch t\u1ea1o r\u1ec5 cho th\u1ea5y: IBA c\u00f3 t\u00e1c d\u1ee5ng m\u1ea1nh m\u1ebd \u0111\u1ebfn qu\u00e1 tr\u00ecnh t\u1ea1o r\u1ec5, t\u1ef7 l\u1ec7 ra r\u1ec5 c\u1ee7a b\u1ea1ch \u0111\u00e0n khi \u0111\u01b0\u1ee3c k\u00edch th\u00edch t\u1ea1o r\u1ec5 b\u1eb1ng m\u00f4i tr\u01b0\u1eddng 1\/2MS*<\/sup> + IBA 1,5 mg\/l + 0,1 ABT mg\/l c\u00e1c ph\u1ee5 gia kh\u00e1c t\u1ef7 l\u1ec7 \u0111\u1ea1t t\u1eeb 72% \u2013 80%<\/p>\n

      Nh\u01b0 v\u1eady ph\u01b0\u01a1ng ph\u00e1p ra r\u1ec5 trong l\u1ecd cho t\u1ef7 l\u1ec7 ra r\u1ec5 t\u01b0\u01a1ng \u0111\u1ed1i cao \u0111\u1ed1i v\u1edbi h\u1ea7u h\u1ebft c\u00e1c \u0111\u1ed1i t\u01b0\u1ee3ng. Ri\u00eang \u0111\u1ed1i v\u1edbi L\u00e1t hoa th\u00ec t\u1ef7 l\u1ec7 ra r\u1ec5 b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p n\u00e0y t\u01b0\u01a1ng \u0111\u1ed1i th\u1ea5p v\u00e0 \u0111\u00f2i h\u1ecfi th\u1eddi gian ra r\u1ec5 k\u00e9o d\u00e0i.<\/p>\n

      IV.4. Ra r\u1ec5 b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p ch\u1ea5m thu\u1ed1c b\u1ed9t th\u01b0\u01a1ng ph\u1ea9m (TTG1- g\u1ed1c l\u00e0 IBA)<\/b><\/p>\n

      V\u1edbi L\u00e1t hoa v\u00e0 Keo lai, \u0111\u1ec1 t\u00e0i \u0111\u00e3 ti\u1ebfn h\u00e0nh th\u1eed nghi\u1ec7m c\u1ea3i ti\u1ebfn ph\u01b0\u01a1ng ph\u00e1p ra r\u1ec5 tr\u1ef1c ti\u1ebfp cho c\u00e1c \u0111\u1ed1i t\u01b0\u1ee3ng n\u00e0y b\u01b0\u1edbc \u0111\u1ea7u cho k\u1ebft qu\u1ea3 t\u1ed1t. C\u00e1c ch\u1ed3i thu \u0111\u01b0\u1ee3c sau qu\u00e1 tr\u00ecnh nh\u00e2n c\u00f3 \u0111\u1ee7 ti\u00eau chu\u1ea9n \u0111\u01b0\u1ee3c x\u1eed l\u00fd ch\u1ea5m thu\u1ed1c b\u1ed9t TTG1, c\u1ea5y v\u00e0o b\u1ea7u \u0111\u1ea5t ho\u1eb7c lu\u1ed1ng c\u00e1t t\u1ea1i v\u01b0\u1eddn \u01b0\u01a1m, \u0111\u01b0\u1ee3c ch\u0103m s\u00f3c nh\u01b0 ph\u01b0\u01a1ng ph\u00e1p gi\u00e2m hom th\u00f4ng th\u01b0\u1eddng, ph\u01b0\u01a1ng ph\u00e1p n\u00e0y c\u00f3 \u01b0u \u0111i\u1ec3m l\u00e0 kh\u00f4ng c\u1ea7n ti\u1ebfn h\u00e0nh ra r\u1ec5 trong ph\u00f2ng th\u00ed nghi\u1ec7m n\u00ean r\u00fat ng\u1eafn \u0111\u01b0\u1ee3c th\u1eddi gian nh\u00e2n gi\u1ed1ng v\u00e0 ti\u1ebft ki\u1ec7m v\u1eadt t\u01b0, nh\u00e2n c\u00f4ng, v\u00ec v\u1eady \u0111\u00e3 gi\u1ea3m gi\u00e1 th\u00e0nh s\u1ea3n xu\u1ea5t c\u00e2y con c\u0169ng nh\u01b0 t\u1ea1o \u0111\u01b0\u1ee3c s\u1ed1 l\u01b0\u1ee3ng c\u00e2y l\u1edbn (\u0110o\u00e0n Th\u1ecb Mai v\u00e0 c\u1ed9ng s\u1ef1, 2009a v\u00e0 2009b)<\/p>\n

      B\u1ea3ng 3. K\u1ebft qu\u1ea3 ra r\u1ec5 b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p ch\u1ea5m thu\u1ed1c b\u1ed9t (TTG)<\/i><\/p>\n\n\n\n\n\n\n
      \n

      \u0110\u1ed1i t\u01b0\u1ee3ng nghi\u00ean c\u1ee9u<\/b><\/p>\n<\/td>\n

      		\n

      Thu\u1ed1c k\u00edch th\u00edch ra r\u1ec5 (TTG1)<\/b><\/p>\n<\/td>\n

      		\n

      T\u00fb l\u00d6 ra r\u00d4 (%)<\/b><\/p>\n<\/td>\n

      		\n

      S<\/b>\u1ed1<\/b> r\u00d4 trung b\u00d7nh<\/b><\/p>\n<\/td>\n<\/tr>\n

      Keo lai t\u1ef1 nhi\u00ean (BV71, BV73, BV75)<\/td>\n\n

      1 %<\/p>\n<\/td>\n

      		\n

      75- 86<\/p>\n<\/td>\n

      		\n

      4 \u2013 6<\/p>\n<\/td>\n<\/tr>\n

      Keo lai nh\u00e2n t\u1ea1o (MA2)<\/td>\n\n

      0,75 %<\/p>\n<\/td>\n

      		\n

      66 – 80<\/p>\n<\/td>\n

      		\n

      3 \u2013 5<\/p>\n<\/td>\n<\/tr>\n

      L\u00e1t hoa (L\u00e1t Ngh\u1ec7 An, l\u00e1t hoa Th\u00e1i Lan)<\/td>\n\n

      1,5 %<\/p>\n<\/td>\n

      		\n

      70 – 75<\/p>\n<\/td>\n

      		\n

      3 \u2013 5<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      K\u1ebeT LU\u1eacN<\/h1>\n

      \u0110\u1ed1i v\u1edbi Keo lai t\u1ef1 nhi\u00ean<\/h2>\n

      1. Ph\u01b0\u01a1ng ph\u00e1p kh\u1eed tr\u00f9ng th\u00edch h\u1ee3p cho c\u00e1c d\u00f2ng Keo lai t\u1ef1 nhi\u00ean m\u1edbi \u0111\u01b0\u1ee3c ch\u1ecdn t\u1ea1o (BV71, BV73, BV75) l\u00e0 HgCl2<\/sub> v\u1edbi n\u1ed3ng \u0111\u1ed9 0,1% v\u00e0 th\u1eddi gian kh\u1eed tr\u00f9ng th\u00edch h\u1ee3p l\u00e0 8-10 ph\u00fat.<\/p>\n

      3. M\u00f4i tr\u01b0\u1eddng nh\u00e2n ch\u1ed3i th\u00edch h\u1ee3p cho c\u00e1c d\u00f2ng Keo lai nghi\u00ean c\u1ee9u l\u00e0 m\u00f4i tr\u01b0\u1eddng MS1* c\u00f3 b\u1ed5 sung IBA n\u1ed3ng \u0111\u1ed9 1,5mg\/l.<\/p>\n

      4. M\u00f4i tr\u01b0\u1eddng ti\u1ec1n ra r\u1ec5 hay m\u00f4i tr\u01b0\u1eddng n\u00e2ng cao ch\u1ea5t l\u01b0\u1ee3ng ch\u1ed3i cho c\u00e1c d\u00f2ng Keo lai th\u00ed nghi\u1ec7m l\u00e0 m\u00f4i tr\u01b0\u1eddng MS1* c\u00f3 b\u1ed5 sung BAP n\u1ed3ng \u0111\u1ed9 1,5mg\/l k\u1ebft h\u1ee3p v\u1edbi NAA n\u1ed3ng \u0111\u1ed9 0,5mg\/l.<\/p>\n

      5. M\u00f4i tr\u01b0\u1eddng t\u1ea1o r\u1ec5 th\u00edch h\u1ee3p cho c\u00e1c d\u00f2ng Keo lai nghi\u00ean c\u1ee9u l\u00e0 1\/2MS1* c\u00f3 b\u1ed5 sung IBA n\u1ed3ng \u0111\u1ed9 1,5mg\/l.<\/p>\n

      6. C\u00e1c ch\u1ed3i ra r\u1ec5 \u0111\u01b0\u1ee3c ch\u0103m s\u00f3c v\u00e0 hu\u1ea5n luy\u1ec7n theo c\u00e1c k\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u v\u1ec1 nh\u00e2n gi\u1ed1ng Keo lai tr\u01b0\u1edbc \u0111\u00e2y cho t\u1ef7 l\u1ec7 s\u1ed1ng cao tr\u00ean 90%, c\u00e2y con c\u00f3 sinh tr\u01b0\u1edfng t\u1ed1t, kho\u1ebb m\u1ea1nh.<\/p>\n

      7. Trong c\u00e1c d\u00f2ng Keo lai th\u00ed nghi\u1ec7m, d\u00f2ng BV73 l\u00e0 d\u00f2ng c\u00f3 kh\u1ea3 n\u0103ng nh\u00e2n ch\u1ed3i cao h\u01a1n so v\u1edbi d\u00f2ng BV71 v\u00e0 BV75.<\/p>\n

      V\u1edbi Keo lai nh\u00e2n t\u1ea1o<\/h2>\n

      1. C\u00f4ng th\u1ee9c kh\u1eed tr\u00f9ng th\u00edch h\u1ee3p cho d\u00f2ng Keo lai nh\u00e2n t\u1ea1o l\u00e0 HgCl2<\/sub> v\u1edbi n\u1ed3ng \u0111\u1ed9 0,1% v\u00e0 th\u1eddi gian kh\u1eed tr\u00f9ng 10 -12 ph\u00fat. Theo c\u00e1ch n\u00e0y, thu \u0111\u01b0\u1ee3c 48,15% m\u1eabu s\u1ea1ch v\u00e0 19% s\u1ed1 m\u1eabu c\u00f3 ch\u1ed3i<\/p>\n

      3. M\u00f4i tr\u01b0\u1eddng nh\u00e2n ch\u1ed3i th\u00edch h\u1ee3p cho d\u00f2ng Keo lai nghi\u00ean c\u1ee9u l\u00e0 MS* c\u00f3 b\u1ed5 sung BAP n\u1ed3ng \u0111\u1ed9 1,5mg\/l.<\/p>\n

      4. M\u00f4i tr\u01b0\u1eddng ti\u1ec1n ra r\u1ec5\u00a0 hay m\u00f4i tr\u01b0\u1eddng n\u00e2ng cao ch\u1ea5t l\u01b0\u1ee3ng ch\u1ed3i cho c\u00e1c d\u00f2ng nghi\u00ean c\u1ee9u l\u00e0 m\u00f4i tr\u01b0\u1eddng MS2* c\u00f3 b\u1ed5 sung BAP n\u1ed3ng \u0111\u1ed9 1,5mg\/l k\u1ebft h\u1ee3p v\u1edbi n\u1ed3ng \u0111\u1ed9 NAA n\u1ed3ng \u0111\u1ed9 0,5mg\/l.<\/p>\n

      5. M\u00f4i tr\u01b0\u1eddng t\u1ea1o r\u1ec5 th\u00edch h\u1ee3p cho c\u00e1c d\u00f2ng Keo lai nh\u00e2n t\u1ea1o nghi\u00ean c\u1ee9u l\u00e0 \u00bd MS2* b\u1ed5 sung IBA n\u1ed3ng \u0111\u1ed9 IBA 1,5mg\/l.<\/p>\n

      6. C\u00e1c ch\u1ed3i ra r\u1ec5 \u0111\u01b0\u1ee3c ch\u0103m s\u00f3c theo c\u00e1c k\u1ebft qu\u1ea3 v\u1ec1 nghi\u00ean c\u1ee9u v\u1ec1 nh\u00e2n gi\u1ed1ng Keo lai nh\u00e2n t\u1ea1o tr\u01b0\u1edbc \u0111\u00e2y cho t\u1ef7 l\u1ec7 s\u1ed1ng cao tr\u00ean 85% c\u00e2y con sinh tr\u01b0\u1edfng t\u1ed1t, kho\u1ebb m\u1ea1nh.<\/p>\n

      V\u1edbi B\u1ea1ch \u0111\u00e0n lai nh\u00e2n t\u1ea1o<\/h2>\n

      1. Ch\u1ea5t kh\u1eed tr\u00f9ng m\u1eabu t\u1ed1t nh\u1ea5t l\u00e0 HgCl2<\/sub> n\u1ed3ng \u0111\u1ed9 0,1%, th\u1eddi gian kh\u1eed tr\u00f9ng l\u00e0 10 ph\u00fat.<\/p>\n

      2. M\u00f9a v\u1ee5 th\u00edch h\u1ee3p v\u00e0 cho t\u1ef7 l\u1ec7 n\u1ea9y ch\u1ed3i cao nh\u1ea5t l\u00e0 th\u00e1ng 4-9 v\u1edbi t\u1ef7 l\u1ec7 m\u1eabu n\u1ea9y ch\u1ed3i l\u1ea7n l\u01b0\u1ee3t l\u00e0 15,76% v\u00e0 16,05% sau 27 ng\u00e0y nu\u00f4i c\u1ea5y.<\/p>\n

      4. M\u00f4i tr\u01b0\u1eddng t\u1ea1o ch\u1ed3i v\u00e0 nh\u00e2n ch\u1ed3i th\u00edch h\u1ee3p l\u00e0: MS3*<\/sup> + 1,0 mg\/l BAP + 0,5 mg\/l NAA + 0,5 mg\/l Kinetin + 30 g\/l \u0111\u01b0\u1eddng Sucrose + 5g\/l agar, pH=5,8.<\/p>\n

      5. M\u00f4i tr\u01b0\u1eddng t\u1ea1o c\u00e2y ho\u00e0n ch\u1ec9nh ra r\u1ec5 l\u00e0 1\/2MS3*<\/sup> + 2,0 mg\/l IBA + 0,5 mg\/l ABT1 + 15 g\/l \u0111\u01b0\u1eddng Sucrose + 5 g\/l agar, pH = 5,8.<\/p>\n

      6. Th\u1eddi gian hu\u1ea5n luy\u1ec7n c\u00e2y con t\u1ed1t tr\u01b0\u1edbc khi c\u1ea5y \u1edf v\u01b0\u1eddn \u01b0\u01a1m l\u00e0 8 – 16 ng\u00e0y.<\/p>\n

      \u0110\u1ed1i v\u1edbi B\u1ea1ch \u0111\u00e0n uro<\/h2>\n

      1. Ph\u01b0\u01a1ng ph\u00e1p kh\u1eed tr\u00f9ng m\u1eabu t\u1ed1t nh\u1ea5t l\u00e0 HgCl2<\/sub> n\u1ed3ng \u0111\u1ed9 0,1%, th\u1eddi gian kh\u1eed tr\u00f9ng\u00a0 8-10 ph\u00fat.<\/p>\n

      2. M\u00f4i tr\u01b0\u1eddng t\u1ea1o ch\u1ed3i th\u00edch h\u1ee3p l\u00e0 m\u00f4i tr\u01b0\u1eddng MS4* c\u00f3 b\u1ed5 sung th\u00eam BAP v\u1edbi n\u1ed3ng \u0111\u1ed9 0,5mg\/l, th\u1eddi gian c\u1ea5y chuy\u1ec3n th\u00edch h\u1ee3p l\u00e0 10 \u2013 12 ng\u00e0y\/ l\u1ea7n.<\/p>\n

      3. M\u00f4i tr\u01b0\u1eddng t\u1ea1o r\u1ec5 ph\u00f9 h\u1ee3p l\u00e0 m\u00f4i tr\u01b0\u1eddng 1\/2MS4* c\u00f3 b\u1ed5 sung IBA\u00a0 n\u1ed3ng \u0111\u1ed9 1,5mg\/ l\u00edt.<\/p>\n

      \u0110\u1ed1i v\u1edbi L\u00e1t hoa<\/h2>\n

      1. \u0110\u00e3 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c k\u1ef9 thu\u1eadt kh\u1eed tr\u00f9ng t\u1ea1o m\u1eabu s\u1ea1ch th\u00edch h\u1ee3p cho hai d\u00f2ng L\u00e1t hoa (NA3 v\u00e0 TL3): S\u1eed d\u1ee5ng ch\u1ea5t HgCl2<\/sub> n\u1ed3ng \u0111\u1ed9 1% kh\u1eed tr\u00f9ng trong 15 ph\u00fat v\u1edbi t\u1ef7 l\u1ec7 m\u1eabu s\u1ea1ch l\u00e0 60,37%<\/p>\n

      2. M\u00f4i tr\u01b0\u1eddng nh\u00e2n ch\u1ed3i th\u00edch h\u1ee3p cho L\u00e1t hoa (NA3 v\u00e0 TL3) l\u00e0 WPM*<\/sup> + 1,0mg\/l BAP v\u1edbi t\u1ef7 l\u1ec7 6,45 \u2013 6,48 ch\u1ed3i\/c\u1ee5m sau 20 ng\u00e0y.<\/p>\n

      3. M\u00f4i tr\u01b0\u1eddng t\u1ea1o r\u1ec5 Invitro <\/i>th\u00edch h\u1ee3p l\u00e0 \u00a0WPM* <\/sup>+ 1,0mg\/l IBA c\u00f3 t\u1ef7 l\u1ec7 \u0111\u1ea1t ch\u1ed3i ra r\u1ec5 \u0111\u1ea1t 93,33% (NA3) v\u00e0 89,63% (TL3) sau 20-30 ng\u00e0y c\u1ea5y.<\/p>\n

      \u0110\u00e3 x\u00e1c \u0111\u1ecbnh \u0111\u01b0\u1ee3c ph\u01b0\u01a1ng ph\u00e1p ra r\u1ec5 ch\u1ed3i Invitro b\u1eb1ng thu\u1ed1c b\u1ed9t TTG cho Keo lai v\u00e0 L\u00e1t hoa<\/h2>\n

      – V\u1edbi keo lai, cho ra r\u1ec5 ch\u1ed3i non Invitro b\u1eb1ng thu\u1ed1c b\u1ed9t TTG n\u1ed3ng \u0111\u1ed9 1,5%, trong th\u1eddi gian t\u1eeb th\u00e1ng 2 \u0111\u1ebfn th\u00e1ng 6 v\u00e0 t\u1eeb th\u00e1ng 9 \u0111\u1ebfn th\u00e1ng 11 l\u00e0 th\u00edch h\u1ee3p.<\/p>\n

      – Khi s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p n\u00e0y, t\u1ef7 l\u1ec7 ra r\u1ec5 cho Keo lai \u0111\u1ea1t \u0111\u1ebfn tr\u00ean 95%, \u0111\u1eb7c bi\u1ec7t th\u1eddi gian cho qu\u00e1 tr\u00ecnh ra r\u1ec5 v\u00e0 hu\u1ea5n luy\u1ec7n c\u00e2y\u00a0 r\u00fat ng\u1eafn \u0111\u01b0\u1ee3c t\u1eeb 10-15 ng\u00e0y (30 ng\u00e0y so v\u1edbi 40-45 ng\u00e0y so v\u1edbi ph\u01b0\u01a1ng ph\u00e1p ra r\u1ec5 trong l\u1ecd).<\/p>\n

      – \u0110\u1ed1i v\u1edbi L\u00e1t hoa, cho ra r\u1ec5 ch\u1ed3i non Invitro b\u1eb1ng thu\u1ed1c b\u1ed9t TTG n\u1ed3ng \u0111\u1ed9 1,0%, trong th\u1eddi gian t\u1eeb th\u00e1ng 1 \u0111\u1ebfn th\u00e1ng 3 v\u00e0 t\u1eeb th\u00e1ng 7 \u0111\u1ebfn th\u00e1ng 10 l\u00e0 th\u00edch h\u1ee3p, th\u1eddi gian ra r\u1ec5 v\u00e0o \u00a0m\u00f9a xu\u00e2n \u2013h\u00e8 l\u00e0 15-20 ng\u00e0y c\u00f2n thu-\u0111\u00f4ng l\u00e0 20-30 ng\u00e0y<\/p>\n

      – Khi s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p n\u00e0y, t\u1ef7 l\u1ec7 ra r\u1ec5 \u0111\u1ea1t \u0111\u1ebfn tr\u00ean 93%, \u0111\u1eb7c bi\u1ec7t th\u1eddi gian cho qu\u00e1 tr\u00ecnh ra r\u1ec5 v\u00e0 hu\u1ea5n luy\u1ec7n c\u00e2y ch\u1ec9 l\u00e0 25-30 ng\u00e0y, r\u00fat ng\u1eafn \u0111\u01b0\u1ee3c t\u1eeb 15-20 ng\u00e0y (so v\u1edbi 40-45 ng\u00e0y so v\u1edbi ph\u01b0\u01a1ng ph\u00e1p ra r\u1ec5 trong l\u1ecd).<\/p>\n","protected":false},"excerpt":{"rendered":"

      \u0110\u1eb6T V\u1ea4N \u0110\u1ec0 Nh\u1eefng n\u0103m g\u1ea7n \u0111\u00e2y, vi\u1ec7c \u1ee9ng d\u1ee5ng ti\u1ebfn b\u1ed9 k\u1ef9 thu\u1eadt v\u1ec1 c\u00f4ng ngh\u1ec7 t\u1ebf b\u00e0o th\u1ef1c v\u1eadt trong c\u00f4ng t\u00e1c ch\u1ecdn gi\u1ed1ng c\u00e2y r\u1eebng \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n t\u1ea1i nhi\u1ec1u n\u01b0\u1edbc tr\u00ean th\u1ebf gi\u1edbi. \u1ede Vi\u1ec7t Nam nu\u00f4i c\u1ea5y m\u00f4 \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n t\u1eeb nh\u1eefng n\u0103m 70. Tuy nhi\u00ean, c\u00e1c […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[13,72],"tags":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/13678"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=13678"}],"version-history":[{"count":3,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/13678\/revisions"}],"predecessor-version":[{"id":13682,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/13678\/revisions\/13682"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=13678"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/categories?post=13678"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/tags?post=13678"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}