{"id":13025,"date":"2014-05-28T10:37:25","date_gmt":"2014-05-28T03:37:25","guid":{"rendered":"http:\/\/vafs.gov.vn\/vn\/?p=13025"},"modified":"2014-05-26T10:50:18","modified_gmt":"2014-05-26T03:50:18","slug":"bao-cao-tom-tat-de-tai-nghien-cuu-lai-giong-nhom-loai-xoan-de-tao-giong-moi-co-nhung-dac-diem-uu-viet","status":"publish","type":"post","link":"https:\/\/vafs.gov.vn\/vn\/bao-cao-tom-tat-de-tai-nghien-cuu-lai-giong-nhom-loai-xoan-de-tao-giong-moi-co-nhung-dac-diem-uu-viet\/","title":{"rendered":"B\u00e1o c\u00e1o t\u00f3m t\u1eaft \u0111\u1ec1 t\u00e0i: Nghi\u00ean c\u1ee9u lai gi\u1ed1ng nh\u00f3m lo\u00e0i xoan \u0111\u1ec3 t\u1ea1o gi\u1ed1ng m\u1edbi c\u00f3 nh\u1eefng \u0111\u1eb7c \u0111i\u1ec3m \u01b0u vi\u1ec7t"},"content":{"rendered":"

I. \u0110\u1eb6T V\u1ea4N \u0110\u1ec0<\/b><\/p>\n

Meliaceae<\/i> l\u00e0 h\u1ecd xoan ph\u1ea7n l\u1edbn l\u00e0 c\u00e2y g\u1ed7, l\u00e1 th\u01b0\u1eddng k\u00e9p 1 l\u1ea7n l\u00f4ng chim, \u00edt khi l\u00e1 \u0111\u01a1n m\u1ecdc c\u00e1ch, r\u1ea5t g\u1ea7n v\u1edbi h\u1ecd Cam\u00a0 (Rutaceae<\/i>) v\u00e0 c\u00f3 nhi\u1ec1u \u0111i\u1ec3m chung v\u1edbi h\u1ecd Thanh th\u1ea5t \u00a0(Simaroubaceae<\/i> ), nh\u01b0ng hoa th\u01b0\u1eddng l\u01b0\u1ee1ng t\u00ednh, nh\u1ecb th\u01b0\u1eddng h\u1ee3p th\u00e0nh \u1ed1ng….<\/p>\n

Chi Azadirachta<\/i><\/b> c\u00f3 2 lo\u00e0i l\u00e0 Azadirachta excelsa <\/i>v\u00e0 Azadirachta indica\u00a0 <\/b><\/i>c\u00f2n chi Melia<\/i><\/b> c\u00f3 3 lo\u00e0i l\u00e0 Melia azadarach, Melia dubi <\/i>v\u00e0 Melia toosendan.<\/i><\/p>\n

Azadirachta excelsa <\/i>l\u00e0 t\u00ean khoa h\u1ecdc c\u1ee7a <\/i>c\u00e2y Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn t\u00ean \u0111\u1ecba ph\u01b0\u01a1ng l\u00e0 C\u00f3c h\u00e0nh l\u00e0 lo\u00e0i c\u00e2y \u0111\u1eb7c bi\u1ec7t trong ki\u1ec3u r\u1eebng kh\u1ed9p kh\u00f4 h\u1ea1n c\u1ee7a v\u00f9ng Ninh Thu\u1eadn. \u0110\u00f3 l\u00e0 lo\u00e0i c\u00e2y \u0111\u00e2m ch\u1ed3i n\u1ea9y l\u1ed9c v\u00e0 c\u00f3 t\u00e1n l\u00e1 xanh \u0111\u1eadm v\u00e0o m\u00f9a kh\u00f4, trong khi c\u00e1c lo\u00e0i kh\u00e1c lu\u00f4n r\u1ee5ng l\u00e1 v\u00e0o m\u00f9a n\u00e0y. \u0110\u00e2y l\u00e0 m\u1ed9t lo\u1ea1i c\u00e2y \u0111a m\u1ee5c \u0111\u00edch, c\u00f3 gi\u00e1 tr\u1ecb kinh t\u1ebf cao, v\u1edbi \u0111\u1eb7c t\u00ednh \u01b0a s\u00e1ng, chu k\u1ef3 kinh doanh t\u01b0\u01a1ng \u0111\u1ed1i ng\u1eafn so lo\u00e0i c\u00e2y b\u1ea3n \u0111\u1ecba kh\u00e1c, d\u1ec5 g\u00e2y tr\u1ed3ng tr\u00ean \u0111\u1ea5t ngh\u00e8o v\u00e0 n\u01a1i c\u00f3 kh\u00ed h\u1eadu kh\u00f4 h\u1ea1n. N\u00f3 \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 l\u00e0 m\u1ed9t trong nh\u1eefng lo\u1ea1i c\u00e2y ch\u1ee7 l\u1ef1c ph\u1ee5c\u00a0 v\u1ee5 cho c\u00f4ng t\u00e1c tr\u1ed3ng r\u1eebng \u1edf v\u00f9ng kh\u00f4 h\u1ea1n t\u1ec9nh Ninh Thu\u1eadn.<\/p>\n

Melia azedarach <\/i>Linn l\u00e0 t\u00ean khoa h\u1ecdc c\u1ee7a c\u00e2y Xoan ta <\/i>hay c\u00f2n \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 Th\u1ea7u \u0111\u00e2u, S\u1ea7u \u0111\u00f4ng, S\u1ea7u \u0111\u00e2u, ph\u00e2n b\u1ed1 ch\u1ee7 y\u1ebfu \u1edf Vi\u1ec7t Nam, L\u00e0o v\u00e0 Campuchia. \u1ede Vi\u1ec7t Nam Xoan ta m\u1ecdc t\u1ef1 nhi\u00ean ho\u1eb7c \u0111\u01b0\u1ee3c tr\u1ed3ng \u1edf h\u1ea7u kh\u1eafp c\u00e1c t\u1ec9nh th\u00e0nh t\u1eeb B\u1eafc t\u1edbi Nam, l\u00e0 lo\u00e0i c\u00e2y \u01b0a s\u00e1ng, \u01b0a kh\u00ed h\u1eadu n\u00f3ng – \u1ea9m, ch\u1ecbu \u0111\u01b0\u1ee3c r\u00e9t, th\u00edch \u1ee9ng v\u1edbi nhi\u1ec1u lo\u1ea1i \u0111\u1ea5t, t\u1eeb chua \u0111\u1ebfn ki\u1ec1m. L\u00e0 lo\u00e0i c\u00e2y m\u1ecdc r\u1ea5t nhanh, chi\u1ec1u cao c\u00f3 th\u1ec3 \u0111\u1ebfn 20 m, \u0111\u01b0\u1eddng k\u00ednh kho\u1ea3ng 60 cm. G\u1ed7 Xoan ta thu\u1ed9c g\u1ed7 nh\u00f3m V, g\u1ed7 xoan c\u00f3 l\u00f5i m\u00e0u h\u1ed3ng hay n\u00e2u nh\u1ea1t, d\u00e1c x\u00e1m tr\u1eafng, g\u1ed7 nh\u1eb9 m\u1ec1m c\u00f3 kh\u1ed1i l\u01b0\u1ee3ng 565 kg\/m3<\/sup>, l\u1ef1c k\u00e9o ngang th\u1edb 22 kg\/cm2, n\u00e9n d\u1ecdc th\u1edb 339 kg\/cm2<\/sup>. G\u1ed7 Xoan ta sau khi ng\u00e2m kh\u00f3 b\u1ecb m\u1ed1i m\u1ecdt, n\u00ean g\u1ed7 Xoan ta th\u01b0\u1eddng d\u00f9ng trong x\u00e2y d\u1ef1ng, trang tr\u00ed n\u1ed9i th\u1ea5t v\u00e0 \u0111i\u00eau kh\u1eafc…<\/p>\n

Nghi\u00ean c\u1ee9u ch\u1ecdn gi\u1ed1ng v\u00e0 lai gi\u1ed1ng gi\u1eefa 2 lo\u00e0i Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn (Azadirachta excelsa<\/i>) thu\u1ed9c chi Azadirachta<\/i><\/b> v\u00e0 lo\u00e0i Xoan ta (Melia azadarach<\/i>) thu\u1ed9c <\/i>\u00a0chi Melia<\/i><\/b> nh\u1eb1m ph\u1ed1i k\u1ebft h\u1ee3p \u0111\u01b0\u1ee3c nh\u1eefng \u0111\u1eb7c t\u00ednh \u01b0u vi\u1ec7t c\u1ee7a b\u1ed1 m\u1eb9 v\u00e0o con lai \u0111\u00e3 \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n b\u1edfi\u00a0 \u0111\u1ec1\u00a0 t\u00e0i \u201cNghi\u00ean c\u1ee9u lai gi\u1ed1ng nh\u00f3m lo\u00e0i xoan \u0111\u1ec3 t\u1ea1o gi\u1ed1ng m\u1edbi c\u00f3 nh\u1eefng \u0111\u1eb7c \u0111i\u1ec3m \u01b0u vi\u1ec7t\u201d.<\/b><\/i><\/p>\n

\u00a0\"xoan\"<\/a><\/p>\n

II. M\u1ee4C TI\u00caU, N\u1ed8I DUNG V\u00c0 PH\u01af\u01a0NG PH\u00c1P NGHI\u00caN C\u1ee8U<\/b><\/p>\n

1. M\u1ee5c ti\u00eau nghi\u00ean c\u1ee9u:<\/b><\/p>\n

Ch\u1ecdn t\u1ea1o m\u1ed9t s\u1ed1 gi\u1ed1ng xoan lai, gi\u1ed1ng b\u1ed1 m\u1eb9 c\u00f3 sinh tr\u01b0\u1edfng nhanh h\u00ecnh d\u00e1ng th\u00e2n \u0111\u1eb9p<\/p>\n

2. N\u1ed9i dung v\u00e0 ph\u01b0\u01a1ng ph\u00e1p nghi\u00ean c\u1ee9u <\/b><\/p>\n

a. N\u1ed9i dung nghi\u00ean c\u1ee9u\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/b><\/p>\n

1. Ch\u1ecdn l\u1ecdc c\u00e1c c\u00e2y tr\u1ed9i cho c\u00e1c lo\u00e0i xoan.<\/i><\/b><\/p>\n

–\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Ch\u1ecdn l\u1ecdc c\u00e2y tr\u1ed9i cho Xoan ta (Melia azadarach)<\/i>\u00a0 v\u00e0\u00a0 C\u00f3c H\u00e0nh (Azadirachta excelsa<\/i>)<\/p>\n

–\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Ph\u00e2n t\u00edch \u0111a d\u1ea1ng di truy\u1ec1n c\u1ee7a m\u1ed9t s\u1ed1 ngu\u1ed3n gi\u1ed1ng ch\u1ecdn l\u1ecdc \u0111\u1ec3 lai gi\u1ed1ng.<\/p>\n

2. Lai gi\u1ed1ng<\/i><\/b><\/p>\n

–\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 X\u00e1c \u0111\u1ecbnh th\u1eddi k\u1ef3 n\u1edf hoa, k\u1ebft qu\u1ea3 c\u1ee7a m\u1ed9t s\u1ed1 lo\u00e0i xoan s\u1eed d\u1ee5ng\u00a0 cho lai gi\u1ed1ng.<\/p>\n

–\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Nghi\u00ean c\u1ee9u lai gi\u1ed1ng kh\u00e1c chi gi\u1eefa Xoan ta (Melia azadarach)<\/i>\u00a0 v\u00e0\u00a0 C\u00f3c H\u00e0nh (Azadirachta excelsa<\/i>)<\/p>\n

3. Kh\u1ea3o nghi\u1ec7m gi\u1ed1ng lai v\u00e0 c\u00e1c c\u00e2y b\u1ed1 m\u1eb9<\/i><\/b><\/p>\n

–\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 So s\u00e1nh sinh tr\u01b0\u1edfng c\u1ee7a c\u00e2y lai v\u1edbi gi\u1ed1ng s\u1ea3n xu\u1ea5t \u0111\u1ea1i tr\u00e0 v\u00e0 b\u1ed1 m\u1eb9.<\/p>\n

4. Nghi\u00ean c\u1ee9u nh\u00e2n gi\u1ed1ng sinh d\u01b0\u1ee1ng (gh\u00e9p, hom) cho c\u00e1c gi\u1ed1ng lai m\u1edbi <\/i><\/b><\/p>\n

b. Ph\u01b0\u01a1ng ph\u00e1p nghi\u00ean c\u1ee9u<\/b><\/p>\n

* Ch\u1ecdn l\u1ecdc c\u00e2y tr\u1ed9i:<\/i><\/b><\/p>\n

– Ch\u1ecdn l\u1ecdc c\u00e2y tr\u1ed9i theo quy ph\u1ea1m x\u00e2y d\u1ef1ng r\u1eebng gi\u1ed1ng v\u00e0 v\u01b0\u1eddn gi\u1ed1ng (QPN 15 \u2013 93). quy ph\u1ea1m k\u1ef9 thu\u1eadt x\u00e2y d\u1ef1ng r\u1eebng gi\u1ed1ng chuy\u1ec3n ho\u00e1 (QPN 16- 93) .<\/p>\n

* Nghi\u00ean c\u1ee9u lai gi\u1ed1ng:<\/i><\/b><\/p>\n

– Thu th\u1eadp v\u00e0 b\u1ea3o qu\u1ea3n h\u1ea1t ph\u1ea5n theo ph\u01b0\u01a1ng ph\u00e1p c\u1ee7a Moncur (1995).<\/p>\n

– Lai gi\u1ed1ng (th\u1ee5 ph\u1ea5n c\u00f3 ki\u1ec3m so\u00e1t) theo ph\u01b0\u01a1ng ph\u00e1p c\u1ee7a Moncur (1995)<\/p>\n

* Ph\u01b0\u01a1ng ph\u00e1p b\u1ed1 tr\u00ed kh\u1ea3o nghi\u1ec7m gi\u1ed1ng:<\/i><\/b><\/p>\n

B\u1ed1 tr\u00ed kh\u1ea3o nghi\u1ec7m v\u00e0 x\u1eed l\u00fd s\u1ed1 li\u1ec7u theo William v\u00e0 Matheson (1994).<\/p>\n

* Ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch \u0111a d\u1ea1ng di truy\u1ec1n <\/i><\/b>theo ph\u01b0\u01a1ng ph\u00e1p CTAB c\u1ee7a Saghai Maroof<\/p>\n

III. K\u1ebeT QU\u1ea2 NGHI\u00caN C\u1ee8U V\u00c0 TH\u1ea2O LU\u1eacN<\/b><\/p>\n

PH\u1ea6N I: C\u00c1C NGHI\u00caN C\u1ee8U C\u01a0 S\u1ede PH\u1ee4C V\u1ee4 CHO CH\u1eccN GI\u1ed0NG V\u00c0 LAI GI\u1ed0NG<\/b><\/p>\n

    \n
  1. A.\u00a0\u00a0\u00a0 <\/b>CH\u1eccN L\u1eccC C\u00c2Y TR\u1ed8I V\u00c0 PH\u00c2N T\u00cdCH \u0110A D\u1ea0NG DI TRUY\u1ec0N<\/b><\/li>\n<\/ol>\n

    1. Ch\u1ecdn l\u1ecdc c\u00e2y tr\u1ed9i ( Xoan ta v\u00e0 Xoan ch\u1ecbu h\u1ea1n Ninh thu\u1eadn) l\u00e0m c\u00e2y b\u1ed1 m\u1eb9<\/b><\/p>\n

    Theo ti\u00eau chu\u1ea9n \u0111\u00e1nh gi\u00e1 ph\u00e2n c\u1ea5p c\u00e2y tr\u1ed9i, \u0111\u1ec1 t\u00e0i \u0111\u00e3 ch\u1ecdn \u0111\u01b0\u1ee3c 82 c\u00e2y tr\u1ed9i c\u00f3 h\u00ecnh d\u00e1ng th\u00e2n th\u1eb3ng \u0111\u1eb9p, tr\u00f2n \u0111\u1ec1u v\u00e0 chi\u1ec1u cao d\u01b0\u1edbi c\u00e0nh \u0111\u1ec1u \u0111\u1ea1t tr\u00ean 5 m v\u00e0 27 c\u00e2y tr\u1ed9i c\u00f3 chi\u1ec1u cao d\u01b0\u1edbi 5m \u1edf c\u00e1c t\u1ec9nh ph\u00eda B\u1eafc. C\u00e1c c\u00e2y tr\u1ed9i n\u00e0y \u0111\u1ec1u \u0111\u01b0\u1ee3c thu h\u1ea1t \u0111\u1ec3 ti\u1ebfn h\u00e0nh kh\u1ea3o nghi\u1ec7m. H\u1ea1t c\u00e1c c\u00e2y tr\u1ed9i n\u00e0y \u0111\u1ec1u c\u00f3 kh\u1ed1i l\u01b0\u1ee3ng bi\u1ebfn \u0111\u1ed9ng l\u1edbn t\u1eeb 13,74g \u0111\u1ebfn 59,74g tr\u00ean 100 h\u1ea1t. Tuy nhi\u00ean theo d\u00f5i sinh tr\u01b0\u1edfng c\u00e1c c\u00e2y n\u00e0y trong v\u01b0\u1eddn \u01b0\u01a1m v\u00e0 \u1edf hi\u1ec7n tr\u01b0\u1eddng kh\u1ea3o nghi\u1ec7m trong giai \u0111o\u1ea1n \u0111\u1ea7u cho th\u1ea5y c\u00e1c c\u00e2y c\u00f3 kh\u1ed1i l\u01b0\u1ee3ng 100 h\u1ea1t l\u1edbn ch\u01b0a ph\u1ea3i \u0111\u00e3 cho sinh tr\u01b0\u1edfng nhanh h\u01a1n c\u00e1c c\u00e2y c\u00f3 kh\u1ed1i l\u01b0\u1ee3ng 100 h\u1ea1t nh\u1ecf.<\/p>\n

    C\u00f2n \u1edf t\u1ec9nh B\u00ecnh \u0110\u1ecbnh ch\u1ecdn \u0111\u01b0\u1ee3c 57 c\u00e2y tr\u1ed9i thu\u1ed9c 20 x\u00e3 l\u00e0 Canh V\u0129nh, C\u00e1t B\u00ecnh, C\u00e1t Hi\u1ec7p, C\u00e1t Minh, Nh\u01a1n h\u00f2a, Nh\u01a1n L\u1ed9c, Nh\u01a1n Th\u1ecd, An T\u00e2n, An H\u00f2a, T\u00e2y Xu\u00e2n, T\u00e2y Giang, T\u00e2y B\u00ecnh, B\u00ecnh Th\u00e0nh, B\u00ecnh H\u00f2a, V\u0129nh Hi\u1ec7p, V\u0129nh Quang, \u00c2n \u0110\u1ee9c, \u00c2n Ngh\u0129a, \u00c2n T\u01b0\u1eddng T\u00e2y, \u00c2n Phong.\u00a0 C\u00e1c c\u00e2y tr\u1ed9i ch\u1ecdn \u1edf B\u00ecnh \u0110\u1ecbnh c\u00f3 c\u1ea5p k\u00ednh t\u01b0\u01a1ng \u0111\u1ed1i \u0111\u1ec1u h\u01a1n c\u00e1c c\u00e2y tr\u1ed9i ch\u1ecdn \u1edf c\u00e1c t\u1ec9nh ph\u00eda B\u1eafc, tuy nhi\u00ean sinh tr\u01b0\u1edfng \u1edf v\u01b0\u1eddn \u01b0\u01a1m \u1edf nh\u1eefng n\u0103m \u0111\u1ea7u sau khi tr\u1ed3ng r\u1eebng cho th\u1ea5y c\u00e1c c\u00e2y tr\u1ed9i n\u00e0y h\u1ea7u nh\u01b0 c\u00f3 sinh tr\u01b0\u1edfng ch\u1eadm h\u01a1n c\u00e1c c\u00e2y tr\u1ed9i ch\u1ecdn \u1edf \u00a0c\u00e1c t\u1ec9nh ph\u00eda B\u1eafc, m\u00e0u s\u1eafc l\u00e1 c\u0169ng k\u00e9m xanh h\u01a1n ( l\u00e1 c\u1ee7a c\u00e1c c\u00e2y ch\u1ecdn \u1edf ph\u00eda B\u1eafc c\u00f3 m\u00e0u xanh \u0111\u1eadm h\u01a1n l\u00e1 c\u00e1c c\u00e2y ch\u1ecdn \u1edf B\u00ecnh \u0110\u00ecnh).<\/p>\n

    C\u00e1c c\u00e2y tr\u1ed9i \u0111\u01b0\u1ee3c tuy\u1ec3n ch\u1ecdn trong r\u1eebng kh\u1ed9p t\u1ef1 nhi\u00ean kh\u00f4 h\u1ea1n Ninh Thu\u1eadn, c\u00e2y Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn th\u01b0\u1eddng \u0111i c\u00f9ng v\u1edbi c\u00e1c lo\u00e0i c\u00e2y nh\u01b0 C\u0103m xe, C\u00e0 Ch\u00ed, C\u1ea9m Li\u00ean, D\u1ea7u, Ki\u1ebft m\u1ecdt, S\u1eafn \u1ed5i, B\u1eb1ng L\u0103ng, v\u1edbi th\u1ea3m t\u01b0\u01a1i l\u00e0 C\u1ecf l\u00f4ng ch\u00f4ng, t\u00e1o, C\u1ecf h\u00f4i, L\u00e0nh ng\u1ea1ch, \u1edf 2 lo\u1ea1i \u0111\u1ea5t l\u00e0 Feralit v\u00e0 \u0111\u1ea5t x\u00e1m \u1edf 6 x\u00e3 l\u00e0 Ma N\u1edbi, H\u00f2a S\u01a1n thu\u1ed9c huy\u1ec7n Ninh S\u01a1n v\u00e0 x\u00e3 Ph\u01b0\u1edbc Th\u1eafng, Ph\u01b0\u1edbc \u0110\u1ea1i, Ph\u01b0\u1edbc Ch\u00ednh, Ph\u01b0\u1edbc H\u00f2a thu\u1ed9c huy\u1ec7n B\u1eafc \u00c1i t\u1ec9nh Ninh Thu\u1eadn. C\u00e1c c\u00e2y tr\u1ed9i \u0111\u01b0\u1ee3c ch\u1ecdn \u0111\u1ec1u c\u00f3 h\u00ecnh d\u00e1ng th\u00e2n \u0111\u1eb9p v\u00e0 ra hoa k\u1ebft qu\u1ea3 t\u1ed1t, tuy nhi\u00ean \u0111\u01b0\u1eddng k\u00ednh c\u00e1c c\u00e2y tr\u1ed9i \u0111\u01b0\u1ee3c ch\u1ecdn kh\u00f4ng l\u1edbn th\u01b0\u1eddng dao \u0111\u1ed9ng 14 \u2013 40cm, c\u00e1c c\u00e2y tr\u1ed9i ch\u1ecdn \u1edf huy\u1ec7n Ninh S\u01a1n th\u01b0\u1eddng c\u00f3 c\u1ea5p k\u00ednh nh\u1ecf h\u01a1n 20cm, c\u00f2n c\u00e1c c\u00e2y tr\u1ed9i ch\u1ecdn huy\u1ec7n B\u1eafc \u00c1i s\u1ed1 c\u00e2y c\u00f3 c\u1ea5p k\u00ednh tr\u00ean 25cm nhi\u1ec1u h\u01a1n. C\u00e1c c\u00e2y tr\u1ed9i c\u00f3 \u0111\u01b0\u1eddng k\u00ednh l\u1edbn tr\u00ean 40cm h\u1ea7u nh\u01b0 \u0111\u00e3 b\u1ecb\u00a0 ng\u01b0\u1eddi d\u00e2n khai th\u00e1c. Chi\u1ec1u cao d\u01b0\u1edbi c\u00e0nh c\u1ee7a c\u00e1c c\u00e2y tr\u1ed9i \u0111\u01b0\u1ee3c ch\u1ecdn c\u0169ng dao \u0111\u1ed9ng t\u1eeb 5,5 m \u2013 10 m ( b\u1ea3ng\u00a0 8 )<\/p>\n

    2. M\u1ed9t s\u1ed1 \u0111\u1eb7c \u0111i\u1ec3m v\u1ec1 n\u1edf hoa, th\u1ee5 ph\u1ea5n \u1edf c\u00e1c lo\u00e0i xoan \u0111\u01b0\u1ee3c nghi\u00ean c\u1ee9u<\/b><\/b><\/p>\n

    2.1 Th\u1eddi k\u1ef3 n\u1edf hoa c\u1ee7a c\u00e1c lo\u00e0i xoan \u0111\u01b0\u1ee3c d\u00f9ng trong lai gi\u1ed1ng<\/b><\/b><\/p>\n

    Nghi\u00ean c\u1ee9u th\u1eddi k\u1ef3 n\u1edf hoa c\u1ee7a c\u00e1c lo\u00e0i Xoan ta, Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn t\u1ea1i H\u00e0 N\u1ed9i, Ninh Thu\u1eadn (2006 – 2008)\u00a0 cho th\u1ea5y c\u00e1c lo\u00e0i n\u00e0y c\u00f3 th\u1eddi gian h\u00ecnh th\u00e0nh n\u1ee5 hoa, n\u1edf hoa v\u00e0 qu\u1ea3 ch\u00edn kh\u00e1c nhau kh\u00e1 r\u00f5 r\u1ec7t. C\u00f3 th\u1ec3 l\u1ea5y s\u1ed1 li\u1ec7u thu th\u1eadp n\u0103m 2007 l\u00e0m th\u00ed d\u1ee5:<\/p>\n

    –\u00a0 Xoan ta c\u00f3 th\u1eddi k\u1ef3 b\u1eaft \u0111\u1ea7u h\u00ecnh th\u00e0nh n\u1ee5 khi v\u1eeba k\u1ebft th\u00fac n\u1ea9y l\u1ed9c cu\u1ed1i th\u00e1ng 1 \u0111\u1ea7u th\u00e1ng 2, th\u1eddi gian n\u1edf hoa t\u1eeb ng\u00e0y 22 th\u00e1ng 2 \u0111\u1ebfn ng\u00e0y 29 th\u00e1ng 3. Th\u1eddi k\u1ef3 n\u1edf hoa k\u00e9o d\u00e0i tr\u00ean 1 th\u00e1ng, th\u1eddi gian qu\u1ea3 ch\u00edn th\u00e1ng 11 v\u00e0 12 c\u00f9ng n\u0103m, khi ch\u00edn qu\u1ea3 v\u1eabn treo tr\u00ean c\u00e2y \u0111\u00e2y l\u00e0 \u0111\u1eb7c \u0111i\u1ec3m thu\u1eadn l\u1ee3i \u0111\u1ec3 thu h\u00e1i qu\u1ea3 ch\u00edn. Th\u1eddi gian t\u1eeb l\u00fac h\u00ecnh th\u00e0nh n\u1ee5 \u0111\u1ebfn l\u00fac qu\u1ea3 ch\u00edn k\u00e9o d\u00e0i 11 th\u00e1ng.<\/p>\n

    – Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn b\u1eaft \u0111\u1ea7u h\u00ecnh th\u00e0nh n\u1ee5 \u0111\u1ea7u th\u00e1ng 2, n\u1edf hoa ng\u00e0y 5 th\u00e1ng 4 \u0111\u1ebfn 3 th\u00e1ng 5, th\u1eddi k\u1ef3 n\u1edf hoa k\u00e9o d\u00e0i g\u1ea7n 1 th\u00e1ng, th\u1eddi gian qu\u1ea3 ch\u00edn th\u00e1ng 7, th\u1eddi gian l\u00fac h\u00ecnh th\u00e0nh n\u1ee5 \u0111\u1ebfn l\u00fac qu\u1ea3 ch\u00edn l\u00e0 5\u00a0 th\u00e1ng.<\/p>\n

    Nh\u01b0 v\u1eady gi\u1eefa Xoan ta v\u00e0 Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn kh\u00f4ng c\u00f3 th\u1eddi gian n\u1edf hoa c\u00f9ng nhau. V\u00ec th\u1ebf, mu\u1ed1n t\u1ea1o ra gi\u1ed1ng lai gi\u1eefa 2 lo\u00e0i n\u00e0y ph\u1ea3i thu h\u1ea1t ph\u1ea5n c\u1ea5t tr\u1eef v\u00e0 d\u00f9ng ph\u01b0\u01a1ng ph\u00e1p th\u1ee5 ph\u1ea5n c\u00f3 ki\u1ec3m so\u00e1t, n\u00f3i c\u00e1ch kh\u00e1c, ph\u1ea3i ti\u1ebfn h\u00e0nh lai gi\u1ed1ng nh\u00e2n t\u1ea1o.<\/p>\n

    2.2 \u1ea2nh h\u01b0\u1edfng c\u1ee7a ph\u01b0\u01a1ng th\u1ee9c th\u1ee5 ph\u1ea5n \u0111\u1ebfn ph\u00e1t tri\u1ec3n c\u1ee7a h\u1ea1t<\/b><\/p>\n

    X\u00e1c \u0111\u1ecbnh kh\u1ea3 n\u0103ng s\u1eed d\u1ee5ng bao c\u00e1ch ly trong qu\u00e1 tr\u00ecnh lai gi\u1ed1ng c\u0169ng nh\u01b0 kh\u1ea3 n\u0103ng t\u1ef1 th\u1ee5 ph\u1ea5n khi ch\u1ee5p bao c\u00e1ch ly l\u00e0 m\u1ed9t vi\u1ec7c c\u1ea7n thi\u1ebft trong lai\u00a0 gi\u1ed1ng c\u00f3 ki\u1ec3m so\u00e1t. S\u1ed1 li\u1ec7u nghi\u00ean c\u1ee9u cho th\u1ea5y \u1edf c\u00e1c c\u00f4ng th\u1ee9c th\u1ee5 ph\u1ea5n c\u00f3 ki\u1ec3m so\u00e1t c\u1ee7a c\u00e1c lo\u00e0i xoan c\u00f3 t\u1ec9 l\u1ec7 \u0111\u1eadu qu\u1ea3 r\u1ea5t th\u1ea5p, cao nh\u1ea5t ch\u1ec9 \u0111\u1ea1t 16,6% v\u00e0 th\u1ea5p nh\u1ea5t ch\u1ec9 \u0111\u1ea1t 0,1%. Trong 79 ph\u00e9p lai c\u00f3 10 ph\u00e9p lai trong lo\u00e0i l\u00e0 \u0111\u1eadu qu\u1ea3 v\u00e0 19 ph\u00e9p lai kh\u00e1c chi c\u0169ng \u0111\u1eadu qu\u1ea3, t\u1ed5ng s\u1ed1 h\u1ea1t thu \u0111\u01b0\u1ee3c l\u00e0 689 h\u1ea1t. Sinh tr\u01b0\u1edfng c\u00e1c c\u00e2y lai kh\u00e1c chi c\u0169ng nh\u01b0 lai trong lo\u00e0i kh\u00f4ng c\u00f3 g\u00ec kh\u00e1c bi\u1ec7t, tuy nhi\u00ean c\u00e1c c\u00e1c c\u00e2y lai trong t\u1ed5 h\u1ee3p lai n\u00e0y ch\u1ec9 th\u1ec3 hi\u1ec7n c\u00f3 \u01b0u th\u1ebf \u1edf giai \u0111o\u1ea1n v\u01b0\u1eddn \u01b0\u01a1m c\u00f2n khi ra r\u1eebng tr\u1ed3ng ch\u01b0a th\u1ea5y bi\u1ec3u hi\u1ec7n c\u1ee7a \u01b0u th\u1ebf lai v\u1ec1 sinh tr\u01b0\u1edfng so v\u1edbi c\u00e1c gi\u1ed1ng b\u1ed1 m\u1eb9 c\u1ee7a ch\u00fang.<\/p>\n

    H\u00ecnh th\u00e1i l\u00e1 c\u1ee7a c\u00e2y lai kh\u00e1c chi ch\u1ecbu \u1ea3nh h\u01b0\u1edfng c\u1ee7a t\u1ebf b\u00e0o ch\u1ea5t, hay n\u00f3i c\u00e1ch kh\u00e1c l\u00e0 khi c\u00e2y m\u1eb9 l\u00e0 Xoan ta c\u00f2n c\u00e2y b\u1ed1 l\u00e0 Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn th\u00ec l\u00e1 c\u1ee7a c\u00e2y lai nghi\u00eang v\u1ec1 m\u1eb9 t\u1ee9c l\u00e0 l\u00e1 k\u00e9p l\u00f4ng chim 2 l\u1ea7n, c\u00f2n khi m\u1eb9 l\u00e0 Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn b\u1ed1 l\u00e0 Xoan ta c\u00e2y lai c\u00f3 l\u00e1 k\u00e9p l\u00f4ng chim 1 l\u1ea7n, gi\u1ed1ng l\u00e1 c\u1ee7a c\u00e2y m\u1eb9. C\u00e1c nghi\u00ean c\u1ee9u v\u1ec1 s\u1ef1 h\u00ecnh th\u00e0nh l\u00e1 v\u00e0 ph\u00e2n h\u00f3a l\u00e1 c\u1ee7a c\u00e2y lai m\u1eb9 l\u00e0 Xoan ta b\u1ed1 l\u00e0 Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn v\u00e0 ng\u01b0\u1ee3c l\u1ea1i cho th\u1ea5y kh\u00f4ng c\u00f3 g\u00ec kh\u00e1c bi\u1ec7t v\u1ec1 s\u1ef1 h\u00ecnh th\u00e0nh l\u00e1<\/p>\n

    3. Ph\u00e2n t\u00edch \u0111a d\u1ea1ng di truy\u1ec1n c\u00e1c xu\u1ea5t x\u1ee9 Xoan ta v\u00e0 Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn<\/p>\n

    –\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 K\u1ebft qu\u1ea3 cho th\u1ea5y v\u1ec1 \u0111\u1ed9 d\u00e0i c\u1ee7a s\u1ea3n ph\u1ea9m PCR \u1edf c\u00e1c c\u00e2y tr\u1ed9i Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn v\u1edbi c\u1ea3 2 m\u1ed3i l\u1ee5c l\u1ea1p \u0111\u1ec1u nh\u1eadn \u0111\u01b0\u1ee3c ph\u00e2n \u0111o\u1ea1n \u0111\u1eb7c tr\u01b0ng v\u00e0 kh\u00f4ng c\u00f3 \u0111a h\u00ecnh. \u0110i\u1ec1u n\u00e0y cho th\u1ea5y m\u1ed1i quan h\u1ec7 di truy\u1ec1n r\u1ea5t g\u1ea7n nhau c\u1ee7a c\u00e1c c\u00e2y tr\u1ed9i \u0111\u00e3 ch\u1ecdn l\u1ecdc. \u0110\u1ec3 c\u00f3 th\u1ec3 ph\u00e2n t\u00edch s\u00e2u h\u01a1n v\u1ec1 s\u1ef1 kh\u00e1c nhau gi\u1eefa c\u00e1c c\u00e2y tr\u1ed9i \u0111\u00e3 ch\u1ecdn l\u1ecdc trong x\u00e2y d\u1ef1ng v\u01b0\u1eddn gi\u1ed1ng h\u1eefu t\u00ednh v\u00e0 v\u00f4 t\u00ednh, s\u1ea3n ph\u1ea9m PCR v\u1edbi m\u1ed3i l\u1ee5c l\u1ea1p atp<\/i>B \u2013 rbc<\/i>L \u0111\u01b0\u1ee3c c\u1eaft v\u1edbi enzym TaqI v\u1edbi m\u1ee5c \u0111\u00edch t\u00ecm ra s\u1ef1 kh\u00e1c nhau trong tr\u00ecnh t\u1ef1 ADN, k\u1ebft qu\u1ea3 th\u1ec3 hi\u1ec7n trong h\u00ecnh 5. Sau khi c\u1eaft enzym c\u0169ng kh\u00f4ng xu\u1ea5t hi\u1ec7n \u0111a h\u00ecnh, \u0111i\u1ec1u n\u00e0y cho th\u1ea5y m\u1ed1i quan h\u1ec7 di truy\u1ec1n r\u1ea5t g\u1ea7n nhau c\u1ee7a c\u00e1c c\u00e2y tr\u1ed9i Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn trong r\u1eebng t\u1ef1 nhi\u00ean \u1edf 6 x\u00e3 c\u1ee7a 2 huy\u1ec7n thu\u1ed9c t\u1ec9nh Ninh Thu\u1eadn.<\/p>\n

    –\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Qua c\u00e1c k\u1ebft qu\u1ea3 ph\u00e2n t\u00edch v\u1edbi c\u00e1c m\u1ed3i RAPD v\u00e0 l\u1ee5c l\u1ea1p cho th\u1ea5y ch\u1ec9 c\u00f3 s\u1ef1 kh\u00e1c bi\u1ec7t nh\u1ecf \u1edf m\u1ee9c \u0111\u1ed9 gen nh\u00e2n<\/b>, c\u00f3 th\u1ec3 l\u00e0 do t\u00e1c \u0111\u1ed9ng c\u1ee7a \u0111i\u1ec1u ki\u1ec7n sinh th\u00e1i l\u00ean c\u00e1c t\u00ednh tr\u1ea1ng th\u00edch nghi c\u1ee7a t\u1eebng v\u00f9ng c\u1ee5 th\u1ec3.<\/p>\n

    3.2 Ph\u00e2n t\u00edch \u0111a d\u1ea1ng di truy\u1ec1n c\u00e1c xu\u1ea5t x\u1ee9 Xoan ta<\/b><\/p>\n

    T\u00e1ch chi\u1ebft ADN t\u1ed5ng s\u1ed1 t\u1eeb l\u00e1 xoan ta<\/i><\/b>: theo ph\u01b0\u01a1ng ph\u00e1p CTAB c\u1ee7a (Doyle v\u00e0 Doyle, 1987) c\u00f3 c\u1ea3i ti\u1ebfn. Ki\u1ec3m tra \u0111\u1ed9 s\u1ea1ch v\u00e0 h\u00e0m l\u01b0\u1ee3ng ADN b\u1eb1ng \u0111o quang ph\u1ed5 h\u1ea5p th\u1ee5 k\u1ebft h\u1ee3p v\u1edbi \u0111i\u1ec7n di tr\u00ean gel agarose 0,9%. K\u1ebft qu\u1ea3 cho th\u1ea5y n\u1ed3ng \u0111\u1ed9 ADN dao \u0111\u1ed9ng t\u1eeb 350 \u0111\u1ebfn 710 ng\/\u00b5l. T\u1ec9 l\u1ec7 OD206<\/sub>\/OD280<\/sub> c\u1ee7a 29 m\u1eabu xoan ta dao \u0111\u1ed9ng t\u1eeb 1,8 \u0111\u1ebfn 2,0. Kho\u1ea3ng bi\u1ebfn \u0111\u1ed9ng n\u00e0y c\u0169ng \u0111\u1ec1u n\u1eb1m trong ph\u1ea1m vi cho ph\u00e9p (1,8 \u2013 2,0). K\u1ebft qu\u1ea3 n\u00e0y kh\u1eb3ng \u0111\u1ecbnh c\u00e1c ADN t\u00e1ch chi\u1ebft \u0111\u01b0\u1ee3c ho\u00e0n to\u00e0n t\u01b0\u01a1ng \u0111\u1ed1i s\u1ea1ch v\u00e0 \u0111\u1ee7 ti\u00eau chu\u1ea9n cho ph\u00e2n t\u00edch ti\u1ebfp theo.<\/p>\n

    B. NH\u1eacN BI\u1ebeT CON LAI V\u00c0 B\u1ed0 M\u1eb8 B\u1eb0NG CH\u1ec8 TH\u1eca L\u1ee4C L\u1ea0P<\/p>\n

    S\u1ea3n ph\u1ea9m PCR\u00a0 gennom c\u1ee7a\u00a0 7 d\u00f2ng c\u00e2y xoan v\u1edbi c\u1eb7p m\u1ed3i 16S sau \u0111\u00f3 \u0111\u01b0\u1ee3c c\u1eaft b\u1eb1ng enzym TaqI c\u00f3 th\u1ec3 cho th\u1ea5y s\u1ef1 kh\u00e1c bi\u1ec7t di truy\u1ec1n c\u1ee7a 1 s\u1ed1 c\u1eb7p con lai v\u1edbi b\u1ed1 m\u1eb9 (h\u00ecnh 18). C\u1ee5 th\u1ec3, s\u1ea3n ph\u1ea9m PCR c\u1ee7a c\u00e2y b\u1ed1 m\u1eb9 l\u00e0 SS28, NT6 khi c\u1eaft b\u1eb1ng enzym TaqI \u0111\u1ec1u cho hai b\u0103ng gi\u1ed1ng nhau c\u00f3 k\u00edch th\u01b0\u1edbc l\u00e0 600bp, 800bp. Tuy nhi\u00ean, \u1edf m\u1eabu con lai NT6SS28 khi c\u1eaft b\u1eb1ng enzym n\u00e0y ngo\u00e0i hai b\u0103ng c\u00f3 k\u00edch th\u01b0\u1edbc gi\u1ed1ng nh\u01b0 c\u1ee7a b\u1ed1 m\u1eb9 (600bp, 800bp), l\u1ea1i c\u00f3 th\u00eam hai b\u0103ng k\u00edch th\u01b0\u1edbc 300bp, 500bp. Nguy\u00ean nh\u00e2n c\u00f3 th\u1ec3 do qu\u00e1 tr\u00ecnh trao \u0111\u1ed5i tr\u00e9o khi k\u1ebft h\u1ee3p ADN c\u1ee7a hai c\u00e2y b\u1ed1 m\u1eb9 trong qu\u00e1 tr\u00ecnh ph\u00e2n b\u00e0o. Nh\u01b0 v\u1eady c\u00f3 th\u1ec3 d\u1ef1a v\u00e0o nh\u1eefng c\u00e2y con lai c\u00f3 b\u1ed1n b\u0103ng k\u00edch th\u01b0\u1edbc: 300bp, 500bp, 600bp, 800bp \u0111\u1ec3 ph\u00e2n bi\u1ec7t\u00a0 v\u1edbi c\u00e2y b\u1ed1 m\u1eb9 ch\u1ec9 c\u00f3 hai b\u0103ng: 600bp, 800bp. T\u01b0\u01a1ng t\u1ef1, s\u1ea3n ph\u1ea9m PCR v\u1edbi c\u1eb7p m\u1ed3i 16S c\u1ee7a ADN c\u00e2y b\u1ed1 m\u1eb9 l\u00e0 NT83\u00a0 v\u00e0 BV22 khi c\u1eaft b\u1eb1ng enzym TaqI c\u0169ng cho hai b\u0103ng gi\u1ed1ng nhau c\u00f3 k\u00edch th\u01b0\u1edbc l\u00e0 600bp, 800bp, nh\u01b0ng \u1edf con lai NT83BV22 ch\u1ec9 cho hai b\u0103ng 300bp, 500bp m\u00e0 l\u1ea1i kh\u00f4ng c\u00f3 hai b\u0103ng 600bp, 800bp nh\u01b0 c\u00e2y b\u1ed1 m\u1eb9. V\u00ec v\u1eady, d\u1ef1a v\u00e0o s\u1ef1 xu\u1ea5t hi\u1ec7n ch\u1ec9 hai b\u0103ng c\u00f3 k\u00edch th\u01b0\u1edbc 300bp, 500bp ch\u01b0a th\u1ec3 ph\u00e2n bi\u1ec7t \u0111\u01b0\u1ee3c con lai v\u00e0 b\u1ed1 m\u1eb9 trong c\u1eb7p lai NT83 v\u00e0 BV22, \u0111\u1ec3 c\u00f3 th\u1ec3 ph\u00e2n bi\u1ec7t \u0111\u01b0\u1ee3c con lai v\u00e0 b\u1ed1 m\u1eb9 c\u1ea7n ph\u00e2n t\u00edch th\u00eam v\u1edbi c\u00e1c m\u1ed3i ch\u1ec9 th\u1ecb ph\u00e2n t\u1eed kh\u00e1c. Nh\u01b0 v\u1eady v\u1edbi c\u1eb7p m\u1ed3i 16S v\u00e0 c\u1eaft b\u1eb1ng enzym TaqI c\u00f3 th\u1ec3 \u00a0ph\u00e2n bi\u1ec7t c\u1eb7p lai SS28 v\u00e0 NT6<\/b>.<\/b><\/b><\/p>\n

    PH\u1ea6N II:\u00a0\u00a0 C\u00c1C K\u1ebeT QU\u1ea2 V\u1ec0 KH\u1ea2O NGHI\u1ec6M GI\u1ed0NG V\u00c0 NH\u00c2N GI\u1ed0NG<\/p>\n

    A<\/b>. SINH TR\u01af\u1edeNG C\u1ee6A C\u00c1C GIA \u0110\u00ccNH XOAN TA V\u00c0 XOAN CH\u1ecaU H\u1ea0N <\/b><\/p>\n

    1. Sinh tr\u01b0\u1edfng c\u1ee7a c\u00e1c gia \u0111\u00ecnh Xoan ta t\u1ea1i \u0110\u1eafc L\u1eafc (8\/06 -12\/09)<\/b><\/p>\n

    2. Sinh tr\u01b0\u1edfng c\u1ee7a c\u00e1c gia \u0111\u00ecnh Xoan ta t\u1ea1i B\u00ecnh \u0110\u1ecbnh (11\/2007 \u2013 11\/2009)<\/b><\/p>\n

    3. Sinh tr\u01b0\u1edfng c\u00e1c gia \u0111\u00ecnh Xoan ch\u1ecbu h\u1ea1n t\u1ea1i Ninh S\u01a1n \u2013 Ninh Thu\u1eadn<\/b><\/p>\n

      \n
    1. B.\u00a0\u00a0\u00a0\u00a0 <\/b>SINH TR\u01af\u1edeNG C\u00c1C GI\u1ed0NG XOAN LAI T\u1ea0I C\u1ea6U HAI PH\u00da TH\u1ecc <\/b><\/li>\n<\/ol>\n

      C. NGHI\u00caN C\u1ee8U V\u1ec0 NH\u00c2N GI\u1ed0NG SINH D\u01af\u1ee0NG<\/b><\/p>\n

      1 NGHI\u00caN C\u1ee8U GH\u00c9P C\u00c2Y C\u00d3C H\u00c0NH<\/b><\/p>\n

      2. TH\u00cd NGHI\u1ec6M NH\u00c2N GI\u1ed0NG C\u00c1C T\u1ed4 H\u1ee2P XOAN LAI B\u1eb0NG HOM<\/b><\/p>\n

      \u00a0<\/b><\/p>\n

      IV. K\u1ebeT LU\u1eacN V\u00c0 KHUY\u1ebeN NGH\u1eca<\/b><\/p>\n

      A. K\u1ebeT LU\u1eacN<\/b><\/p>\n

        \n
      1. \u0110\u1ec1 t\u00e0i \u0111\u00e3 ch\u1ecdn \u0111\u01b0\u1ee3c 167 c\u00e2y tr\u1ed9i Xoan ta v\u00e0 50 c\u00e2y tr\u1ed9i Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn, c\u00e1c c\u00e2y tr\u1ed9i n\u00e0y \u0111\u1ec1u c\u00f3 h\u00ecnh d\u00e1ng th\u00e2n \u0111\u1eb9p v\u00e0 ch\u1ea5t l\u01b0\u1ee3ng c\u00e2y \u0111\u00e1p \u1ee9ng \u0111\u01b0\u1ee3c ti\u00eau chu\u1ea9n c\u1ee7a ch\u1ecdn l\u1ecdc c\u00e2y tr\u1ed9i trong r\u1eebng t\u1ef1 nhi\u00ean v\u00e0 c\u00e2y ph\u00e2n t\u00e1n.\n
          \n
        1. V\u1ec1 ph\u00e2n t\u00edch ch\u1ec9 th\u1ecb ph\u00e2n t\u1eed:<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n

          –\u00a0\u00a0\u00a0\u00a0\u00a0 C\u00e1c gia \u0111\u00ecnh Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn (Azadirachta excelsa<\/i>), Xoan ta (M. azadarach)\u00a0 <\/i>nghi\u00ean c\u1ee9u c\u00f3 m\u1ee9c \u0111\u1ed9 \u0111a d\u1ea1ng di truy\u1ec1n th\u1ea5p.<\/p>\n

          –\u00a0\u00a0\u00a0\u00a0\u00a0 C\u00f3 6 c\u1eb7p gia \u0111\u00ecnh Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn (Azadirachta excelsa<\/i>) c\u00f3 h\u1ec7 s\u1ed1 t\u01b0\u01a1ng \u0111\u1ed3ng g\u1ea7n b\u1eb1ng 1 (t\u1eeb 0,978 \u0111\u1ebfn 0,981) l\u00e0 c\u1eb7p NT38 v\u00e0 NT54, NT8 v\u00e0 NT11, NT53 v\u00e0 NT50, NT13 v\u00e0 NT22, NT19 v\u00e0 NT56, NT42 v\u00e0 NT58.<\/p>\n

            \n
          1. Nghi\u00ean c\u1ee9u v\u1ec1 lai gi\u1ed1ng<\/li>\n<\/ol>\n

            – M\u00f9a n\u1edf hoa c\u1ee7a Xoan ta v\u00e0o th\u00e1ng\u00a0 2-3 qu\u1ea3 ch\u00edn v\u00e0o th\u00e1ng 11-12 c\u00f9ng n\u0103m, c\u00f2n m\u00f9a n\u1edf hoa c\u1ee7a Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn v\u00e0o th\u00e1ng 4-5 qu\u1ea3 ch\u00edn 6-7 c\u00f9ng n\u0103m.<\/p>\n

            – Trong lai gi\u1ed1ng xoan khi kh\u1eed \u0111\u1ef1c v\u00e0 ch\u1ee5p bao c\u00e1ch ly kh\u00f4ng th\u1ea5y \u0111\u1eadu qu\u1ea3. Ch\u1ee5p bao c\u00e1ch ly khi hoa s\u1eafp n\u1edf c\u0169ng kh\u00f4ng th\u1ea5y \u0111\u1eadu qu\u1ea3. \u0110i\u1ec1u \u0111\u00f3 ch\u1ee9ng t\u1ecf c\u00e1c lo\u00e0i xoan tuy c\u00f3 c\u1ea5u t\u1ea1o hoa l\u01b0\u1ee1ng t\u00ednh, song ho\u00e0n to\u00e0n b\u1ea5t th\u1ee5 khi t\u1ef1 th\u1ee5 ph\u1ea5n c\u00f9ng hoa trong bao c\u00e1ch ly. N\u00f3i c\u00e1ch kh\u00e1c vi\u1ec7c ch\u1ee5p bao c\u00e1ch ly tr\u01b0\u1edbc v\u00e0 sau khi th\u1ee5 ph\u1ea5n c\u00f3 ki\u1ec3m so\u00e1t l\u00e0 ho\u00e0n to\u00e0n c\u00f3 t\u00e1c d\u1ee5ng v\u00e0 c\u1ea7n thi\u1ebft trong lai gi\u1ed1ng.<\/p>\n

            – \u0110\u1ec1 t\u00e0i \u0111\u00e3 lai th\u00e0nh c\u00f4ng \u0111\u01b0\u1ee3c 29 t\u1ed5 h\u1ee3p lai trong lo\u00e0i v\u00e0 kh\u00e1c chi, trong \u0111\u00f3 c\u00f3 19 t\u1ed5 h\u1ee3p lai l\u00e0 kh\u00e1c chi ( hai chi l\u00e0 Azadirachta <\/i>v\u00e0 Melia c\u1ee7a 2 lo\u00e0i l\u00e0 A. excelsa v\u00e0 M. azadarach )<\/i>t\u1ed5ng s\u1ed1 h\u1ea1t thu \u0111\u01b0\u1ee3c l\u00e0 689 h\u1ea1t lai.<\/i><\/p>\n

            – <\/i>C\u00f3 th\u1ec3 d\u1ef1a v\u00e0o c\u1eb7p m\u1ed3i 16S v\u00e0 c\u1eaft b\u1eb1ng enzym TaqI c\u00f3 th\u1ec3 \u00a0ph\u00e2n bi\u1ec7t con lai NT6SS28 v\u1edbi b\u1ed1 m\u1eb9 c\u1ee7a ch\u00fang (<\/i>SS28 v\u00e0 NT6) <\/b>d\u1ef1a v\u00e0o s\u1ef1 xu\u1ea5t hi\u1ec7n c\u1ee7a hai b\u0103ng c\u00f3 k\u00edch th\u01b0\u1edbc 300bp, 500bp.<\/p>\n

              \n
            1. \u0110\u00e1nh gi\u00e1 v\u1ec1 sinh tr\u01b0\u1edfng c\u00e1c gia \u0111\u00ecnh xoan v\u00e0 gi\u1ed1ng xoan lai<\/li>\n<\/ol>\n

              – T\u1ea1i tu\u1ed5i 3 c\u00f3 th\u1ec3 ch\u1ecdn \u0111\u01b0\u1ee3c 6 gia \u0111\u00ecnh l\u00e0 SS7, BV45, BV28, SS26, BV58 v\u00e0 SS25 c\u00f3 th\u1ec3 t\u00edch th\u00e2n c\u00e2y \u0111\u1ea1t t\u1eeb 25dm3<\/sup>\/c\u00e2y \u0111\u1ebfn 30dm3<\/sup>\/c\u00e2y. T\u0103ng tr\u01b0\u1edfng b\u00ecnh qu\u00e2n v\u1ec1 \u0111\u01b0\u1eddng k\u00ednh \u0111\u1ea1t 3cm\/n\u0103m v\u00e0 chi\u1ec1u cao \u0111\u1ea1t x\u1ea5p x\u1ec9 3m\/n\u0103m.<\/p>\n

              – So s\u00e1nh sinh tr\u01b0\u1edfng gi\u1eefa c\u00e1c c\u00e2y lai v\u00e0 b\u1ed1 m\u1eb9 cho th\u1ea5y t\u1ea1i v\u01b0\u1eddn \u01b0\u01a1m c\u00f3 kh\u00e1c bi\u1ec7t v\u1ec1 sinh tr\u01b0\u1edfng nh\u01b0ng \u1edf r\u1eebng tr\u1ed3ng tr\u00ean 1 n\u0103m ch\u01b0a th\u1ea5y s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 sinh tr\u01b0\u1edfng.<\/p>\n

                \n
              1. V\u1ec1 nh\u00e2n gi\u1ed1ng:<\/li>\n<\/ol>\n

                – V\u1ec1 kh\u1ea3 n\u0103ng nh\u00e2n gi\u1ed1ng (gh\u00e9p): C\u00f3 th\u1ec3 \u00e1p d\u1ee5ng c\u00e1c ph\u01b0\u01a1ng ph\u00e1p gh\u00e9p \u00e1p v\u00e0 gh\u00e9p n\u00eam \u0111\u1ec3 nh\u00e2n gi\u1ed1ng v\u00f4 t\u00ednh c\u00e2y Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn (Azadirachta excelsa<\/i>).<\/p>\n

                – Nh\u1eefng nh\u1eadn \u0111\u1ecbnh s\u01a1 b\u1ed9 v\u1ec1 nghi\u00ean c\u1ee9u nu\u00f4i c\u00e2y m\u00f4 cho c\u00e1c gi\u1ed1ng xoan lai nh\u01b0 sau:<\/p>\n

                + Kh\u1eed tr\u00f9ng b\u1eb3ng HgCl2 n\u1ed3ng \u0111\u1ed9 0.05% th\u1eddi gian 10 ph\u00fat cho t\u1ef7 l\u1ec7 m\u1eabu s\u1ea1ch cao \u0111\u1ea1t tr\u00ean 80%<\/p>\n

                + M\u00f4i tr\u01b0\u1eddng nh\u00e2n ch\u1ed3i c\u00f3 n\u1ed3ng \u0111\u1ed9 BAP 0.2mg\/l v\u00e0 IBA 0.01 mg\/l l\u00e0 m\u00f4i tr\u01b0\u1eddng h\u1eefu hi\u1ec7u c\u1ea3 v\u1ec1 ch\u1ea5t l\u01b0\u1ee3ng, s\u1ed1 l\u01b0\u1ee3ng ch\u1ed3i v\u00e0 \u00fd ngh\u0129a kinh t\u1ebf.<\/p>\n

                + M\u00f4i tr\u01b0\u1eddng ra r\u1ec5 hi\u1ec7u qu\u1ea3 \u1edf n\u1ed3ng \u0111\u1ed9 NAA 1mg\/l v\u00e0 IBA 2mg\/l<\/p>\n

                + Kh\u00f4ng c\u00f3 s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 kh\u1ea3 n\u0103ng nh\u00e2n gi\u1ed1ng gi\u1eefa 3 t\u1ed5 h\u1ee3p lai \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y.<\/p>\n

                B. T\u1ed2N T\u1ea0I<\/b><\/p>\n

                Do th\u1eddi gian c\u00f3 h\u1ea1n n\u00ean \u0111\u1ec1 t\u00e0i m\u1edbi ch\u1ec9 d\u1eebng l\u1ea1i \u1edf vi\u1ec7c \u0111\u00e1nh gi\u00e1 sinh tr\u01b0\u1edfng c\u1ee7a c\u00e1c gia \u0111\u00ecnh Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn, Xoan ta, xoan lai \u00a0\u1edf giai \u0111o\u1ea1n 1- 3 tu\u1ed5i, do v\u1eady nh\u1eefng k\u1ebft lu\u1eadn v\u1ec1 sinh tr\u01b0\u1edfng m\u1edbi c\u00f3 t\u00ednh ch\u1ea5t b\u01b0\u1edbc \u0111\u1ea7u.<\/p>\n

                C. KHUY\u1ebeN NGH\u1eca<\/b><\/p>\n

                  \n
                1. Ti\u1ebfp t\u1ee5c theo d\u00f5i kh\u1ea3o nghi\u1ec7m \u0111\u1ec3 c\u00f3 k\u1ebft lu\u1eadn ch\u00ednh x\u00e1c h\u01a1n v\u1ec1 c\u00e1c gia \u0111\u00ecnh Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn, Xoan ta, xoan lai \u0111\u00e3 \u0111\u01b0\u1ee3c ch\u1ecdn l\u1ecdc.<\/li>\n
                2. \u0110\u00e2y m\u1edbi l\u00e0 c\u00e1c nghi\u00ean c\u1ee9u b\u01b0\u1edbc \u0111\u1ea7u v\u1ec1 \u0111a d\u1ea1ng di truy\u1ec1n (m\u1edbi s\u1eed d\u1ee5ng 6 m\u1ed3i RAPD v\u00e0 2 m\u1ed3i l\u1ee5c l\u1ea1p). \u0110\u1ec3 c\u00f3 th\u1ec3 ph\u00e2n t\u00edch s\u00e2u s\u1eafc v\u00e0 to\u00e0n di\u1ec7n h\u01a1n v\u1ec1 s\u1ef1 kh\u00e1c nhau gi\u1eefa c\u00e1c d\u00f2ng Xoan ch\u1ecbu h\u1ea1n Ninh Thu\u1eadn, n\u00ean ti\u1ebfp t\u1ee5c ph\u00e2n t\u00edch c\u00e1c m\u1eabu v\u1edbi m\u1ed9t s\u1ed1 m\u1ed3i l\u1ee5c l\u1ea1p kh\u00e1c v\u00e0 t\u1ea5t c\u1ea3 c\u00e1c s\u1ea3n ph\u1ea9m PCR v\u1edbi c\u00e1c m\u1ed3i l\u1ee5c l\u1ea1p c\u1ea7n \u0111\u01b0\u1ee3c c\u1eaft v\u1edbi c\u00e1c enzym gi\u1edbi h\u1ea1n v\u1edbi m\u1ee5c \u0111\u00edch t\u00ecm ra s\u1ef1 kh\u00e1c nhau trong tr\u00ecnh t\u1ef1 cpADN \u00a0( chloroplast DNA).<\/li>\n<\/ol>\n

                  \u00a0<\/b><\/p>\n

                  V.<\/b> T\u00c0I LI\u1ec6U THAM KH\u1ea2O: <\/b><\/p>\n

                    \n
                  1. A.K. Singh, S. Chand, S. Pattnaik & P. K. Chand, 2002. Adventitious shoot organogenesis and plant regeneration from cotyledons of Dalbergia sissoo <\/i>Roxb., a timber yielding tree legume. Plant Cell, Tissue and Organ Culture <\/i>68: 203\u2013209.<\/li>\n
                  2. Agro Forestry Tree Database (2006), A tree species reference and selection guide, Azadirachta excelsa<\/i>, http:\/\/www.worldagroforestry.org<\/a><\/span>.<\/li>\n
                  3. Chung SM., Jack ES. (2003), The development and evaluation of consensus chloroplast primer pairs that possess highly variable sequence regions in a diverse array of plant taxa. Theor Appl Genet (2003) 107: 757-767.<\/li>\n
                  4. Devos N., Tyteca D., Raspe O., Wesselingh RA., and Jacquemart AL. (2003), Patterns of chloroplast diversity among western European Dactylorhiza species (Orchidaceae). Plant Syst Evol 243: 85-97<\/li>\n
                  5. Danh m\u1ee5c c\u00e1c lo\u00e0i th\u1ef1c v\u1eadt Vi\u1ec7t Nam t\u1eadp II, nh\u00e0 xu\u1ea5t b\u1ea3n n\u00f4ng nghi\u1ec7p, H\u00e0 N\u1ed9i 2003.<\/li>\n
                  6. Davidson. J, (1998). Domestication and breeding programme for Eucalyptyus in the Asia-Pacific region. Food and agriculture organization of the united nations. Philippines, 252 trang.<\/li>\n
                  7. David F. Marshall. 1999. A preliminary report of the application of AFLP\u00a0 and microsatellitepolymorphism to investagatethe extent and partition of genetic variation in a sample of lines of Melia azdarach. SCRI 4.<\/li>\n
                  8. Fang DQ., Roose ML., Federici CT.\u00a0 (1997),\u00a0 Fingerprinting trifoliate orange germ plasm accessions with isozymes, RFLPs and inter-simple sequence repeat markers.\u00a0 Theoretical and applied genetics 95: 211-219.<\/li>\n
                  9. Ferreira AR., Keim P (1997), Genetic Mapping of Soybean [Glycine max (L.) Merr.] Using Random Amplified Polymorphic DNA (RAPD), Plant Mol Biol Rep, 15(4): 335- 354.<\/li>\n<\/ol>\n

                    10. Heinze Berthold (2007), A database of\u00a0 PCR primers for the chloroplast genomes of higher plants. Plants Methods 2007: 3-4.<\/p>\n

                    11. Nguy\u1ec5n Thu\u00fd H\u1ea1nh (2005), Nghi\u00ean c\u1ee9u m\u1ed1i quan h\u1ec7 di truy\u1ec1n m\u1ed9t s\u1ed1 chi v\u00e0 lo\u00e0i c\u00e2y h\u1ecd d\u1ea7u (Dipterocarpaceae) \u1edf Vi\u1ec7t nam b\u1eb1ng ch\u1ec9 th\u1ecb RAPD v\u00e0 AND l\u1ee5c l\u1ea1p. Lu\u1eadn \u00e1n cao h\u1ecdc.<\/p>\n

                    12. Ph\u1ea1m Ho\u00e0ng H\u1ed9, 1991. C\u00e2y c\u1ecf Vi\u1ec7t Nam. Nh\u00e0 xu\u1ea5t b\u1ea3n Montr\u00e9al.<\/p>\n

                    13. Heinze Berthold (2007), A database of\u00a0 PCR primers for the chloroplast genomes of higher plants. Plants Methods 2007: 3-4.<\/p>\n

                    14. Nguy\u1ec5n Thu\u00fd H\u1ea1nh (2005), Nghi\u00ean c\u1ee9u m\u1ed1i quan h\u1ec7 di truy\u1ec1n m\u1ed9t s\u1ed1 chi v\u00e0 lo\u00e0i c\u00e2y h\u1ecd d\u1ea7u (Dipterocarpaceae) \u1edf Vi\u1ec7t nam b\u1eb1ng ch\u1ec9 th\u1ecb RAPD v\u00e0 AND l\u1ee5c l\u1ea1p. Lu\u1eadn \u00e1n cao h\u1ecdc.<\/p>\n

                    15. Nguy\u1ec5n T\u00edch v\u00e0 Tr\u1ea7n H\u1ee3p , 1971. C\u00e2y r\u1eebng Vi\u1ec7t Nam. Nh\u00e0 xu\u1ea5t b\u1ea3n N\u00f4ng th\u00f4n, H\u00e0 N\u1ed9i.<\/p>\n

                    16. Tr\u1ea7n \u0110\u00ecnh L\u00fd, 1993. 1900 c\u00e2y c\u00f3 \u00edch. Nh\u00e0 XB Th\u1ebf Gi\u1edbi H\u00e0 N\u1ed9i<\/p>\n

                    17. Levi A. and Rowland LJ. (1997), Identifying blueberry cultivars and evaluating their genetic relationships using randomly amplified polymorphic DNA (RAPD) and simple sequence repeat-(SSR-) anchored primers. Journal of the American Society for Horticultural Science 122: 74-78.<\/p>\n

                    18. Lee. S. L, Wickneswari. R, Mahani. M. C, Zakri. A. H. (2000), \u201cMating System parameters in a Tropcal Tree Species, Shorea leprosula Miq. (Dipteerocarpaceae), from Malaysian Lowland Dipterocarp Forest\u201d. Biotropica<\/i> 32(4a): 693 \u2013 702.<\/p>\n

                    19. Mace ES., Lester RN., Gebhardt CG. (1999), AFLP analysis of genetic relationships among the cultivated eggplant, Solanum melongena L., and wild relatives (Solanaceae), Theor. Appl. Genet. 99: 626 – 633.<\/p>\n

                    20. McMillan E., Sun G. (2004), Genetic relationships of tetraploid Elymus speciess and their genomic donor speciess inferred from polymerase chain reaction \u2013 restriction length polymorphism analysis of chloroplast gene regions. Theor Appl Gent 108: 535-542.<\/p>\n

                    21. Mohanty A., Martin JP., Aguinagalde I. (2001), Chloroplast DNA study in wild populations and some cultivars of\u00a0 Prunu avium L. Theor Appl Genet 103: 112-117.<\/p>\n

                    22. Moncur. M. W. (1995). Techiques for Pollinating Eucalypts. ACIAR Technical Reports No.34. 20 trang.<\/p>\n

                    23. Moncur. M. W, Gunn B.V, (1995) Seed\u00a0 development and germination respones of Melia azdarach var. australiasica. Tropical Tree Seed Research. Aciar project No. 28.<\/p>\n

                    24. Nagaoka T. and Ogihara Y. (1997),\u00a0 Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers.\u00a0 Theoretical and applied genetics 94: 597-602.<\/p>\n

                    25. Navascues M., Emerson BC. (2005), Chloroplast microsatellites: measure of genetic diversity and the effect of homoplasy. Molecular Ecology 14: 1333-1341.<\/p>\n

                    26. Nicolosi E, Deng. Z. N, Gentile .A, La Malfa. S, Continella. G, Tribulato. E. (2000), \u201dCitrus phylogeny and genetic origin of important species as investigated by molecular markers\u201d. Theor. Appl. Genet<\/i>., 100, pp. 1155-1166.\u00a0 Vi\u1ec7n \u0110TQH R\u1eebng, 1980-1985. C\u00e2y g\u1ed7 r\u1eebng VN. Nh\u00e0 XBNN H\u00e0 N\u1ed9i<\/p>\n

                    27. Olivier Monteuuis & Marie-Claude Bon, 2000. Influence of auxins and darkness on in vitro <\/i>rooting of micropropagated shoots from mature and juvenile Acacia mangium.<\/i> Plant Cell, Tissue and Organ Culture <\/i>63: 173\u2013177<\/p>\n

                    28. \u0110inh Th\u1ecb Ph\u00f2ng, L\u00ea Tr\u1ea7n B\u00ecnh, L\u00ea Th\u1ecb Mu\u1ed9i, Nguy\u1ec5n Th\u1ecb H\u1ea3i H\u00e0, L\u00ea Duy Th\u00e0nh, Nguy\u1ec5n V\u0103n Vi\u1ebft (2004), Nghi\u00ean c\u1ee9u \u0111a d\u1ea1ng t\u1eadp \u0111o\u00e0n gi\u1ed1ng l\u00faa c\u00f3 t\u00ednh kh\u00e1ng kh\u00e1c nhau v\u1edbi b\u1ec7nh b\u1ea1c l\u00e1 l\u00faa vi khu\u1ea9n Xanthomonas oryzae b\u1eb1ng k\u1ef9 thu\u1eadt RAPD. B\u00e1o c\u00e1o khoa h\u1ecdc H\u1ed9i ngh\u1ecb to\u00e0n qu\u1ed1c v\u1ec1 Nh\u1eefng v\u1ea5n \u0111\u1ec1 nghi\u00ean c\u1ee9u c\u01a1 b\u1ea3n trong khoa h\u1ecdc s\u1ef1 s\u1ed1ng \u0111\u1ecbnh h\u01b0\u1edbng n\u00f4ng l\u00e2m nghi\u1ec7p mi\u1ec1n n\u00fai. Th\u00e1i Nguy\u00ean 23\/9\/2004: 571-574.<\/p>\n

                    29. Pannee Denrungruang (2005), The Composition of Fatty Acids from Azadirachta excelsa Seed<\/i>, <\/b>Forest Products Research and Development Division, Royal Forest Department, Chatuchak, Bangkok 10900, Thailand.<\/p>\n

                    30. Richar. G, Olmstead, Jeffrey. D, Palmer (1994), \u201cChloroplast DNA Systematics: Areview of methods and data anlysis\u201d. American Journal of Botany<\/i>, Vol. 81, No. 9, 1205 \u2013 1224.<\/p>\n

                    31. Rohlf F. J. (1993), \u201cNTSYS-pc Numerical taxonomomy and multivariate analysis system\u201d, Version 1.80. Applied Biostatitics, New York. <\/i><\/p>\n

                      \n
                    1. 32. \u00a0Saghai Maroof M. A., Biyashev R. M., Yang G. P., Zhang Q., Allard R. W, (1994),\u00a0 \u201cExtraodirnarily polymorphic microsatellite DNA in barley: species diversity, chromosome location, and population dynamics\u201d, Proc. <\/i>Natl. Acad. Sci. USA,<\/i> 91, pp. 5466-5470<\/li>\n
                    2. 33. \u00a0Schmutterer H. and M. Doll (1993), The marrango or Phillipine neem tree,<\/i> Azadirachta excelsa<\/i> (= A.integrifoliola<\/i>): A new source of insecticide with growth regulating properties.<\/i> Phytoparasitica, 21(1): 79 – 86.<\/li>\n<\/ol>\n

                      34. Shinoza K., Ohme M., Tanaka M., Wakasugi T., Hayashida N., Matsubayashi T., Zaita N., Chunwongse J., ObokataJ., Yamaguchi-Sinozaki K., Ohto C., Torazawa K., meng BY, Sugita M, Deno H, Kamogashira T, Ya \u2013 mada K, Meng BY, Sugita M. (1986), The complete nucleotide sequence of the tobacco horoplast genome. Plant Mol Biol Rep 4: 110-147.<\/p>\n

                      35. S. Duttagupta, India and Yasuomi Ibaraki, Japan (editors), 2006. Plant Tissue Culture Engineering (trang 15-28). Published by Springer<\/p>\n

                      36. Shinoza K., Ohme M., Tanaka M., Wakasugi T., Hayashida N., Matsubayashi T., Zaita N., Chunwongse J., ObokataJ., Yamaguchi-Sinozaki K., Ohto C., Torazawa K., meng BY, Sugita M, Deno H, Kamogashira T, Ya \u2013 mada K, Meng BY, Sugita M. (1986), The complete nucleotide sequence of the tobacco horoplast genome. Plant Mol Biol Rep 4: 110-147.<\/p>\n

                      37. Tsumura Y., Ohba K.,\u00a0 Strauss SH.\u00a0 (1996), Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica).\u00a0 Theoretical and applied genetics 92: 40-45.<\/p>\n

                      38. V\u1ee5 KHCN, B\u1ed9 NN-PTNT, 2000. T\u00ean c\u00e2y r\u1eebng Vi\u1ec7t Nam. Nh\u00e0 XB N\u00f4ng nghi\u1ec7p, H\u00e0 N\u1ed9i<\/p>\n

                      39. Welsh J., McClelland M. (1991), Fingerprinting genome using PCR with arbitrary primer, Nucleic Acids Res, 18, pp. 7213-7218.<\/p>\n

                      40. Williams J.G.K., Kubelik A.E., Levak K.J., Rafalski J.A., Tingey S.V. (1990), DNA polymorphisms amplified by arbitrary primer are useful as genetic markers, Nucleic Acids Res, 18, pp. 6531-6535.<\/p>\n

                      41. William, E. R and Matheson, A. C., (1994). Experimental Design and Analysis for Use in Tree Improvement. CSIRO, Melbourne and\u00a0\u00a0\u00a0\u00a0 ACIAR, Canberra, 174 trang.<\/p>\n","protected":false},"excerpt":{"rendered":"

                      I. \u0110\u1eb6T V\u1ea4N \u0110\u1ec0 Meliaceae l\u00e0 h\u1ecd xoan ph\u1ea7n l\u1edbn l\u00e0 c\u00e2y g\u1ed7, l\u00e1 th\u01b0\u1eddng k\u00e9p 1 l\u1ea7n l\u00f4ng chim, \u00edt khi l\u00e1 \u0111\u01a1n m\u1ecdc c\u00e1ch, r\u1ea5t g\u1ea7n v\u1edbi h\u1ecd Cam\u00a0 (Rutaceae) v\u00e0 c\u00f3 nhi\u1ec1u \u0111i\u1ec3m chung v\u1edbi h\u1ecd Thanh th\u1ea5t \u00a0(Simaroubaceae ), nh\u01b0ng hoa th\u01b0\u1eddng l\u01b0\u1ee1ng t\u00ednh, nh\u1ecb th\u01b0\u1eddng h\u1ee3p th\u00e0nh \u1ed1ng…. Chi Azadirachta […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[13,72],"tags":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/13025"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=13025"}],"version-history":[{"count":3,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/13025\/revisions"}],"predecessor-version":[{"id":13029,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/posts\/13025\/revisions\/13029"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=13025"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/categories?post=13025"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/tags?post=13025"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}