{"id":21252,"date":"2021-08-31T09:33:47","date_gmt":"2021-08-31T02:33:47","guid":{"rendered":"http:\/\/vafs.gov.vn\/vn\/?page_id=21252"},"modified":"2021-08-31T09:35:27","modified_gmt":"2021-08-31T02:35:27","slug":"quy-trinh-cong-nghe-nhan-sinh-khoi-in-vitro-va-san-xuat-che-pham-am","status":"publish","type":"page","link":"https:\/\/vafs.gov.vn\/vn\/quy-trinh-cong-nghe-nhan-sinh-khoi-in-vitro-va-san-xuat-che-pham-am\/","title":{"rendered":"Quy tr\u00ecnh c\u00f4ng ngh\u1ec7 nh\u00e2n sinh kh\u1ed1i in vitro v\u00e0 s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m AM"},"content":{"rendered":"<p><strong>TH\u00d4NG TIN CHUNG<\/strong><\/p>\n<ul>\n<li>C\u00f4ng nh\u1eadn t\u1ea1i Q\u0110 s\u1ed1 3502\/Q\u0110-BNN-KHCN ng\u00e0y 11 th\u00e1ng 9 n\u0103m 2019 c\u1ee7a B\u1ed9 N\u00f4ng nghi\u1ec7p v\u00e0 Ph\u00e1t tri\u1ec3n n\u00f4ng th\u00f4n.<\/li>\n<li>T\u00e1c gi\u1ea3: L\u00ea Qu\u1ed1c Huy, V\u0169 Qu\u00fd \u0110\u00f4ng, L\u00ea Th\u1ecb Thu H\u1eb1ng, \u0110\u1eb7ng Quang Huy.<\/li>\n<\/ul>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><strong>N\u1ed8I DUNG CH\u00cdNH<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>N\u1ed9i dung c\u1ee7a ti\u1ebfn b\u1ed9 k\u1ef9 thu\u1eadt bao g\u1ed3m 2 Quy tr\u00ecnh ch\u00ednh l\u00e0 (i) Quy tr\u00ecnh c\u00f4ng ngh\u1ec7 nh\u00e2n sinh kh\u1ed1i AM <em>in vitro <\/em>v\u00e0 (ii) Quy tr\u00ecnh c\u00f4ng ngh\u1ec7 s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m AM t\u1eeb sinh kh\u1ed1i AM <em>in vitro. <\/em>S\u01a1 \u0111\u1ed3 t\u1ed5ng th\u1ec3 nh\u01b0 sau:<\/p>\n<p><a href=\"http:\/\/vafs.gov.vn\/vn\/quy-trinh-cong-nghe-nhan-sinh-khoi-in-vitro-va-san-xuat-che-pham-am\/am\/\" rel=\"attachment wp-att-21254\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-full wp-image-21254\" src=\"http:\/\/vafs.gov.vn\/vn\/wp-content\/uploads\/sites\/2\/2021\/08\/AM.jpg\" alt=\"\" width=\"498\" height=\"527\" srcset=\"https:\/\/vafs.gov.vn\/vn\/wp-content\/uploads\/sites\/2\/2021\/08\/AM.jpg 498w, https:\/\/vafs.gov.vn\/vn\/wp-content\/uploads\/sites\/2\/2021\/08\/AM-472x500.jpg 472w\" sizes=\"(max-width: 498px) 100vw, 498px\" \/><\/a><\/p>\n<h3><a name=\"_Toc70512254\"><\/a>1. Quy tr\u00ecnh c\u00f4ng ngh\u1ec7 nh\u00e2n sinh kh\u1ed1i AM in vitro<\/h3>\n<ul>\n<li><a name=\"_Toc67320507\"><\/a> B\u01b0\u1edbc 1. T\u1ea1o v\u00e0 ph\u1ee5c tr\u00e1ng v\u1eadt li\u1ec7u gi\u00e1 th\u1ec3 r\u1ec5 in vitro mang gen Ri-tDNA, g\u1ed3m t\u1ea1o gi\u00e1 th\u1ec3 r\u1ec5 in vitro, \u0111\u1ed3ng nu\u00f4i c\u1ea5y <em> rihizogenses<\/em> v\u1edbi r\u1ec5 c\u00e0 r\u1ed1t in vitro, ch\u1ecdn r\u1ec5 c\u00e0 r\u1ed1t in vitro c\u00f3 Ri \u2013 tDNA tr\u00ean m\u00f4i tr\u01b0\u1eddng ch\u1ecdn l\u1ecdc c\u00f3 kh\u00e1ng sinh v\u00e0 duy tr\u00ec c\u1ea5y chuy\u1ec3n v\u00e0 ph\u1ee5c tr\u00e1ng s\u1ef1 gi\u00e0 h\u00f3a c\u1ee7a r\u1ec5 C\u00e0 r\u1ed1t Ri- tDNA in vitro.<\/li>\n<\/ul>\n<h4>&#8211;\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 B\u01b0\u1edbc 2. T\u1ea1o v\u1eadt li\u1ec7u g\u1ed1c AM in vitro<\/h4>\n<ul>\n<li>B\u01b0\u1edbc 3. T\u1ea1o c\u1ed9ng sinh R\u1ec5 Ri-tDNA \u2013 AM m\u1ed9t l\u1ea7n cho nh\u00e2n sinh kh\u1ed1i AM in vitro nhi\u1ec1u l\u1ea7n<\/li>\n<li>Tr\u00ean \u0111\u0129a th\u1ea1ch nu\u00f4i c\u1ea5y v\u1eadt li\u1ec7u AM v\u00f4 tr\u00f9ng \u0111\u00e3 n\u1ea3y m\u1ea7m, c\u1eaft l\u1ea5y AM sao cho m\u1ea3nh nguy\u00ean li\u1ec7u n\u1eb1m tr\u1ecdn b\u00ean trong ph\u1ea7n th\u1ea1ch c\u00f3 k\u00edch th\u01b0\u1edbc t\u01b0\u01a1ng \u1ee9ng v\u1edbi m\u1ea3nh th\u1ea1ch \u0111\u00e3 c\u1eaft b\u1ecf \u1edf \u0111\u0129a m\u00f4i tr\u01b0\u1eddng. D\u00f9ng dao c\u1ea5y chuy\u1ec3n m\u1ea3nh agar c\u00f3 ch\u1ee9a v\u1eadt li\u1ec7u AM v\u00e0 \u0111\u1eb7t kh\u00edt v\u00e0o ph\u1ea7n kho\u1ea3ng tr\u1ed1ng \u0111\u00e3 lo\u1ea1i b\u1ecf m\u00f4i tr\u01b0\u1eddng MSR tr\u00ean petri.<\/li>\n<li>C\u1ea5y chuy\u1ec3n 3-4 \u0111o\u1ea1n gi\u00e1 th\u1ec3 r\u1ec5 C\u00e0 r\u1ed1t in vitro mang gen Ri-tDNA (d\u00e0i 3-4 cm) l\u00ean \u0111\u0129a th\u1ea1ch v\u00e0 ti\u1ebfp x\u00fac v\u1edbi m\u1ea3nh agar c\u00f3 v\u1eadt li\u1ec7u AM.<\/li>\n<li>Nu\u00f4i c\u1ea5y c\u1ed9ng sinh AM- gi\u00e1 th\u1ec3 r\u1ec5 C\u00e0 r\u1ed1t Ri-tDNA trong \u0111i\u1ec1u ki\u1ec7n t\u1ed1i, \u1edf 26-27<sup>0<\/sup>C trong t\u1ee7 nu\u00f4i c\u1ea5y (Incubator); theo d\u00f5i th\u01b0\u1eddng xuy\u00ean ti\u1ebfn tri\u1ec3n c\u1ee7a gi\u00e1 th\u1ec3 r\u1ec5, s\u1ee3i n\u1ea5m, s\u1ef1 h\u00ecnh th\u00e0nh ph\u00e1t tri\u1ec3n c\u1ed9ng sinh v\u00e0 sinh kh\u1ed1i b\u00e0o t\u1eed, s\u1ee3i n\u1ea5m m\u1edbi tr\u00ean \u0111\u0129a nu\u00f4i c\u1ea5y.<\/li>\n<li>Duy tr\u00ec s\u1eed d\u1ee5ng v\u1eadt li\u1ec7u gi\u00e1 th\u1ec3 r\u1ec5 C\u00e0 r\u1ed1t Ri-tDNA c\u1ed9ng sinh AM trong c\u00e1c \u0111\u0129a petri (sau 1-2 th\u00e1ng nu\u00f4i c\u1ea5y) l\u00e0m nguy\u00ean li\u1ec7u g\u1ed1c cho c\u1ea5y chuy\u1ec3n, nh\u00e2n nu\u00f4i v\u00e0 nh\u00e2n sinh kh\u1ed1i c\u1ed9ng sinh AM in vitro nhi\u1ec1u l\u1ea7n li\u00ean ti\u1ebfp m\u00e0 kh\u00f4ng c\u1ea7n ph\u1ea3i l\u1eb7p l\u1ea1i quy tr\u00ecnh t\u1ea1o c\u1ed9ng sinh AM nh\u01b0 tr\u00ean cho m\u1ed7i l\u1ea7n nh\u00e2n sinh kh\u1ed1i; thu h\u1ed3i sinh kh\u1ed1i c\u1ed9ng sinh AM in vitro trong c\u00e1c \u0111\u0129a petri v\u00e0 b\u00ecnh nh\u00e2n nu\u00f4i sau 4-5 th\u00e1ng nu\u00f4i c\u1ea5y cho s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m AM.<\/li>\n<\/ul>\n<h4>&#8211;\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 B\u01b0\u1edbc 4. Nh\u00e2n sinh kh\u1ed1i c\u1ed9ng sinh AM in vitro cho s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m AM<\/h4>\n<ul>\n<li>Th\u1ef1c hi\u1ec7n c\u1ea5y chuy\u1ec3n v\u1eadt li\u1ec7u g\u1ed1c gi\u00e1 th\u1ec3 r\u1ec5 C\u00e0 r\u1ed1t Ri-tDNA c\u1ed9ng sinh AM t\u1eeb c\u00e1c c\u00e1c \u0111\u0129a petri nguy\u00ean li\u1ec7u sang m\u00f4i tr\u01b0\u1eddng m\u1edbi (MSR c\u1ea3i ti\u1ebfn, 0,5 % agar, 0,7 % sucrose) tr\u00ean c\u00e1c \u0111\u0129a petri \u1d13 9 cm cho nh\u00e2n sinh kh\u1ed1i c\u1ed9ng sinh AM in vitro: 3-5 \u0111o\u1ea1n gi\u00e1 th\u1ec3 r\u1ec5 C\u00e0 r\u1ed1t Ri-tDNA c\u1ed9ng sinh AM (\u0111o\u1ea1n \u0111\u1ea7u r\u1ec5 to, m\u1eadp, m\u00e0u tr\u1eafng, d\u00e0i 3-4 cm).<\/li>\n<li>Nu\u00f4i c\u1ea5y nh\u00e2n sinh kh\u1ed1i c\u1ed9ng sinh AM in trong \u0111i\u1ec1u ki\u1ec7n t\u1ed1i, \u1edf 26-27<sup>0<\/sup>C trong t\u1ee7 nu\u00f4i c\u1ea5y (Incubator) ho\u1eb7c trong ph\u00f2ng nu\u00f4i v\u1edbi \u0111i\u1ec1u ki\u1ec7n \u00e1nh s\u00e1ng, nhi\u1ec7t \u0111\u1ed9 t\u01b0\u01a1ng t\u1ef1.<\/li>\n<li>K\u1ebft qu\u1ea3 sau 3-4 th\u00e1ng nu\u00f4i c\u1ea5y m\u1ed7i \u0111\u0129a petri \u0111\u1ea1t 15.000 &#8211; 18.000 b\u00e0o t\u1eed\/\u0111\u0129a petri.<\/li>\n<\/ul>\n<h3><a name=\"_Toc70512255\"><\/a>2. Quy tr\u00ecnh c\u00f4ng ngh\u1ec7 s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m AM<\/h3>\n<h4>&#8211;\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 B\u01b0\u1edbc 5. Thu h\u1ed3i sinh kh\u1ed1i AM in vitro<\/h4>\n<h4>&#8211;\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 B\u01b0\u1edbc 6. K\u1ef9 thu\u1eadt tr\u1ed9n ph\u1ee5 gia v\u00e0 s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m AM t\u1eeb sinh kh\u1ed1i AM in vitro<\/h4>\n<ul>\n<li>Tr\u1ed9n \u0111\u1ec1u tr\u00ean m\u00e1y tr\u1ed9n v\u1edbi ch\u1ea5t ph\u1ee5 gia apatit, than b\u00f9n v\u00e0 sinh kh\u1ed1i kh\u00f4 AM in vitro theo t\u1ef7 l\u1ec7 h\u1ed7n h\u1ee3p \u0111\u00e3 \u0111\u00e1nh gi\u00e1 v\u00e0 t\u00ednh to\u00e1n.<\/li>\n<li>S\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m g\u1ed1c AM in vitro d\u1ea1ng b\u1ed9t t\u1eeb sinh kh\u1ed1i AM in vitro \u0111\u00e3 l\u00e0m kh\u00f4 &#8211; thu h\u1ed3i sau 3-4 th\u00e1ng nh\u00e2n c\u1ea5y v\u00e0 ch\u1ea5t mang v\u1edbi th\u00e0nh ph\u1ea7n c\u1ea3i ti\u1ebfn nh\u01b0 tr\u00ean, \u0111\u1ea3m b\u1ea3o \u22651000 IP\/g ch\u1ebf ph\u1ea9m.<\/li>\n<li>\u00c1p d\u1ee5ng nh\u00e2n sinh kh\u1ed1i AM in vivo t\u1eeb ch\u1ebf ph\u1ea9m g\u1ed1c \u00e1p d\u1ee5ng cho s\u1ea3n xu\u1ea5t ch\u1ebf ph\u1ea9m AM 100IP\/g ch\u1ebf ph\u1ea9m, nh\u1eb1m h\u1ea1 gi\u00e1 th\u00e0nh s\u1ea3n ph\u1ea9m, hi\u1ec7u qu\u1ea3 v\u00e0 thu\u1eadn ti\u1ec7n s\u1eed d\u1ee5ng theo y\u00eau c\u1ea7u c\u1ee7a th\u1ef1c ti\u1ec5n s\u1ea3n xu\u1ea5t.<\/li>\n<\/ul>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><strong>\u0110\u1ecaA CH\u1ec8 \u1ee8NG D\u1ee4NG<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Cho c\u00e2y keo, c\u00e2y b\u1ea1ch \u0111\u00e0n \u1edf Ph\u00fa Th\u1ecd, Qu\u1ea3ng Tr\u1ecb v\u00e0 c\u00e1c v\u00f9ng kh\u00e1c c\u00f3 \u0111i\u1ec1u ki\u1ec7n t\u01b0\u01a1ng t\u1ef1.<\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><strong>\u0110I\u1ec0U KI\u1ec6N \u1ee8NG D\u1ee4NG<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><em>\u00a0\u00a0<\/em><\/strong>\u00c1p d\u1ee5ng c\u00e2y keo, c\u00e2y b\u1ea1ch \u0111\u00e0n t\u1ea1i c\u00e1c v\u01b0\u1eddn \u01b0\u01a1m v\u00e0 r\u1eebng tr\u1ed3ng.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>TH\u00d4NG TIN CHUNG C\u00f4ng nh\u1eadn t\u1ea1i Q\u0110 s\u1ed1 3502\/Q\u0110-BNN-KHCN ng\u00e0y 11 th\u00e1ng 9 n\u0103m 2019 c\u1ee7a B\u1ed9 N\u00f4ng nghi\u1ec7p v\u00e0 Ph\u00e1t tri\u1ec3n n\u00f4ng th\u00f4n. T\u00e1c gi\u1ea3: L\u00ea Qu\u1ed1c Huy, V\u0169 Qu\u00fd \u0110\u00f4ng, L\u00ea Th\u1ecb Thu H\u1eb1ng, \u0110\u1eb7ng Quang Huy. N\u1ed8I DUNG CH\u00cdNH N\u1ed9i dung c\u1ee7a ti\u1ebfn b\u1ed9 k\u1ef9 thu\u1eadt bao g\u1ed3m 2 Quy tr\u00ecnh ch\u00ednh [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/pages\/21252"}],"collection":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/comments?post=21252"}],"version-history":[{"count":2,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/pages\/21252\/revisions"}],"predecessor-version":[{"id":21255,"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/pages\/21252\/revisions\/21255"}],"wp:attachment":[{"href":"https:\/\/vafs.gov.vn\/vn\/wp-json\/wp\/v2\/media?parent=21252"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}